Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia

Cano-Gómez, Ana; Goulden, Evan F.; Owens, Leigh; Høj, Lone

doi:10.1111/j.1574-6968.2009.01850.x

Cited by 89 publications

(60 citation statements)

References 27 publications

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“…with 99 to 100% sequence identities. In the case of Vibrio isolates, phylogenetic analysis based on the 16S rRNA sequences, and not only BLAST searches, was necessary for the identification at species level, due to the high similarities among species for this gene and the presence of multiple unclassified sequences in the databases (Gomez-Gil et al 2004, Cano-Gómez et al 2010. For isolates belonging to the V. harveyi species group (V. harveyi, V. rotiferianus, V. campbellii and V. owensii) the 16S rRNA gene sequence interspecies similarities ranged from 98.9 to 99.6%.…”

Section: Isolates Identificationmentioning

confidence: 99%

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). I. Disease induction

Rivera-Posada¹,

Pratchett²,

Cano-Gómez³

et al. 2011

Dis. Aquat. Org.

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This is the first report of the successful induction of a transmissible disease in the coral-eating crown-of-thorns starfish Acanthaster planci (COTS). Injection of thiosulfate-citratebile-sucrose agar (TCBS) culture medium into COTS induced a disease characterized by discoloured and necrotic skin, ulcerations, loss of body turgor, accumulation of colourless mucus on many spines especially at their tip, and loss of spines. Blisters on the dorsal integument broke through the skin surface and resulted in large, open sores that exposed the internal organs. Oedema and reddened digestive tissues and destruction of connective fibers were common. Moreover, healthy COTS in contact with these infected animals also displayed signs of disease and died within 24 h. TCBS induced 100% mortality in injected starfish. There was no intro duction of new pathogens into the marine environment. TCBS promoted the growth of COTS' naturally occurring Vibrionales to high densities with subsequent symbiont imbalance followed by disease and death.

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Section: Isolates Identificationmentioning

confidence: 99%

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). I. Disease induction

Rivera-Posada¹,

Pratchett²,

Cano-Gómez³

et al. 2011

Dis. Aquat. Org.

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“…Identification of the reisolated strains was performed by sequencing multiple loci (16S rRNA, topA, and mreB genes) in order to enable discrimination between V. harveyi-like species at the strain level, which cannot reliably be achieved by sequencing the 16S rRNA gene alone (8,9).…”

Section: Discussionmentioning

confidence: 99%

“…In preliminary infection experiments (described below), one of the dominant isolates (DY05) caused 85% mortality of stage 3 phyllosomas 72 h after vectored challenge (data not shown). DY05 was subsequently characterized by exhaustive phenotypical, biochemical, and phylogenetic analyses and determined to belong to the novel species V. owensii, of which DY05 is the type strain (8).…”

Section: Methodsmentioning

confidence: 99%

“…Microhabitat preferences and ecological selection may be key factors in the speciation of vibrios (20), and intensive aquaculture systems are thought to select for bacterial virulence, including traits that enhance infectivity and transmission (29,33). Accordingly, many Vibrio species are pathogenic to cultured crustacean zooplanktonic larval forms, including the three closely related species Vibrio harveyi (32,35), V. campbellii (17,44), and the recently described V. owensii (8).…”

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confidence: 99%

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Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Goulden

Hall

Bourne

et al. 2012

Appl Environ Microbiol

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bThe type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species. Major steps toward the commercialization of closed life cycle aquaculture production of the ornate spiny lobster (Panulirus ornatus) have been reported recently (36), yet nutritional deficits (41) and mortality caused by bacterial disease (5) remain major constraints to hatchery productivity. Mass mortalities of larvae (phyllosomas) are often associated with enteric vibriosis, an infection of the midgut gland (hepatopancreas) caused by Vibrio species (6, 52).Members of the genus Vibrio are natural marine inhabitants, playing important roles in nutrient cycling and forming associations with zooplankton (49). Microhabitat preferences and ecological selection may be key factors in the speciation of vibrios (20), and intensive aquaculture systems are thought to select for bacterial virulence, including traits that enhance infectivity and transmission (29,33). Accordingly, many Vibrio species are pathogenic to cultured crustacean zooplanktonic larval forms, including the three closely related species Vibrio harveyi (32, 35), V. campbellii (17, 44), and the recently described V. owensii (8).It is of paramount importance to the development of efficient disease management strategies that pathogens be identified by using experimental infection models that provide information on infection routes and infection dynamics (38). Recently, microorganisms engineered to express fluorescent proteins (FP) have significantly increased the understanding of invasive pathways and infection dynamics of pathogens, including V. anguillarum, Aeromonas hydrophila, and Edwardsiella tarda in fish models (10, 24, 30) and V. harveyi in abalone (50). Panulirus sp. p...

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“…The Harveyi clade consists of eleven species: V. harveyi, V. campbellii, V. rotiferianus, V. alginolyticus, V. parahaemolyticus, V. mytili, V. natriegens, the newly described V. azureus [29], V. sagamiensis [30], V. owensii [2], V. communis [4] which is a junior synonym of V. owensii [3,8] and most recently, V. jasicida [31]. Many isolates of this clade particularly V. harveyi, V. campbellii and V. owensii, are bioluminescent and contribute to a very important penaeid hatchery disease called luminescent vibriosis.…”

Section: Introductionmentioning

confidence: 99%

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

Busico-Salcedo

Owens

2013

Indian J. Virol.

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Vibrio owensii is one of the most virulent vibrios known being able to kill crustacean larvae at 10 2 CFU ml -1 . This study describes virulence changes to naïve strains of Vibrio harveyi and Vibrio campbellii when infected with the bacteriophage VOB from a closely related species V. owensii 47666-1. The bacteriophage from V. owensii was induced into lytic phase by using mitomycin C at 100 ng ml -1 . One strain of V. harveyi and two strains of V. campbellii from 29 tested containing no prophage were susceptible to lysogenic conversion with VOB. Virulence changes induced in Harveyi clade bacteria included the upregulation of protein secretion, statistically significant increased haemolysin and chitinase production and increased mortality to nauplii of Penaeus monodon. No change in siderophore production was observed. Bacteriophage VOB is likely to be responsible for some of the virulence factors expressed by V. owensii. As this bacteriophage is able to infect strains of V. harveyi and V. campbellii this phage may contribute to increased virulence of other vibrios in aquaculture and in the natural environment.

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Vibrio owensii sp. nov., isolated from cultured crustaceans in Australia

Cited by 89 publications

References 27 publications

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). I. Disease induction

Injection of Acanthaster planci with thiosulfate-citrate-bile-sucrose agar (TCBS). I. Disease induction

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Virulence Changes to Harveyi Clade Bacteria Infected with Bacteriophage from Vibrio owensii

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