Vibrio biofilm inhibitors screened from marine fungi protect shrimp against acute hepatopancreatic necrosis disease (AHPND)

Soowannayan, Chumporn; Teja, D. Nitin Chandra; Yatip, Pattanan; Mazumder, Fahmina Yasmin; Krataitong, Kulatara; Unagul, Panida; Suetrong, Satinee; Preedanon, Sita; Klaysuban, Anupong; Sakayaroj, Jariya; Sangtiean, Tanuwong

doi:10.1016/j.aquaculture.2018.09.004

Cited by 18 publications

(13 citation statements)

References 24 publications

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“…In Fig. 7 a, the pathognomonic symptoms of AHPND such as pale and shrunken HP and empty gut were observed in the V. harveyi infected group 65 ; wherein, these infectious lesions were significantly abridged in the DCTN treated group. This result evidently authorizes the in vivo disease protection efficacy of DCTN against V. harveyi caused AHPND.…”

Section: Resultsmentioning

confidence: 91%

“…Hence, hindering biofilm formation by anti-biofilm agents has considered a promising mode to trim-down the infection rate in aquaculture 23 . Recently, Soowannayan et al 65 have successfully protected AHPND infected shrimps ( P. vannamei ) using Vibrio biofilm inhibitors. Therefore, the antibiofilm activity of the DCTN was assessed using in vitro assay 17 .…”

Section: Resultsmentioning

confidence: 99%

“…Then, the formalin-fixed animals were allowed to sectioning using a microtome and tissue embedding. Finally, the cut sections were subjected to hematoxylin and eosin (H&E) staining and they were observed under a light microscope with an attached digital camera (Nikon Eclipse Ti 100) 65 .…”

Section: Methodsmentioning

confidence: 99%

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Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Alexpandi

Gopi

Durgadevi

et al. 2020

Sci Rep

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Nowadays, bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields. Herein, Diplocyclos palmatus leaf extract-derived green-fluorescence carbon dots (DP-CDs) were prepared using the hydrothermal method. Due to the strong fluorescence stability, the prepared DP-CDs were coated on filter-paper to make a fluorometric sensor-strip for Fe 3+ detection. After, a bandgap-narrowed DP-CDs/TiO 2 nanocomposite (DCTN) was prepared using the methanolic extract of D. palmatus. The prepared DCTN exhibited improved photocatalytic bacterial deactivation under sunlight irradiation. The DCTN-photocatalysis slaughtered V. harveyi cells by the production of reactive oxygen species, which prompting oxidative stress, damaging the cell membrane and cellular constituents. These results suggest the plausible mode of bactericidal action of DCTN-photocatalysis under sunlight. Further, the DCTN has shown potent anti-biofilm activity against V. harveyi , and thereby, DCTN extended the survival of V. harveyi -infected shrimps during the in vivo trial with Litopenaeus vannamei . Notably, this is the first report for the disinfection of V. harveyi -mediated acute-hepatopancreatic necrosis disease (AHPND) using nanocomposite. The reduced internal-colonization of V. harveyi on the hepatopancreas as well as the rescue action of the pathognomonic effect in the experimental animals demonstrated the anti-infection potential of DCTN against V. harveyi -mediated AHPND in aquaculture.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

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Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Alexpandi

Gopi

Durgadevi

et al. 2020

Sci Rep

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“…Out of 39 obligate marine fungi screened, Oceanitis cincinnatula was found to inhibit biofilm formation in V. harveyi VH0 and VH1 the greatest, with 84% and 91% relative biofilm inhibition, respectively, (Soowannayan, Boonmee, et al, 2019; Soowannayan, Chandra Teja, et al, 2019). A recent paper also screened marine endophytic fungi for their biofilm inhibition against V. parahaemolyticus and V. harveyi .…”

Section: Quorum Sensing Inhibitionmentioning

confidence: 99%

Targeting quorum sensing and biofilm formation in the control of Vibrio harveyi infections in Penaeus vannamei

Guzman

Yatip

Soowannayan

et al. 2022

Aquaculture Research

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Vibriosis, a disease caused by Vibrio spp., including V. harveyi, contributes to enormous economic loss to the shrimp aquaculture industry. With the worsening incidences of antimicrobial resistance in aquaculture, novel strategies for combating this bacterial infection are urgently needed. One of these is by targeting the bacterial communication system, called quorum sensing (QS), which is responsible for the expression of genes necessary for bacterial pathogenesis. Recently, bioactive compounds capable of inhibiting QS were identified. This review focuses on the discovery of bioactive substances from animal, plant, and microbial origins with the capability of inhibiting QS and biofilm formation, a mechanism important in the pathogenesis of vibriosis. Furthermore, recent developments in the use of these QS inhibitors (QSIs) as feed supplement for Penaeus vannamei shrimp were also discussed. These findings will provide the necessary information for the discovery, development, and eventual commercialization of these QSIs as tools in mitigating vibriosis in the shrimp industry.

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“…To ensure that food-producing shrimp are sustainable and safe for human consumption, alternative antibiotic treatment methods are required. Studies have shown that natural products such as chitosan and essential oils from microbes, red seaweed, plants, and animals exhibit capability to control bacterial infections in white shrimp and tilapia [ 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Depolymerized Fractions of Sulfated Galactans Extracted from Gracilaria fisheri and Their Antibacterial Activity against Vibrio parahaemolyticus and Vibrio harveyi

et al. 2022

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Various seaweed sulfated polysaccharides have been explored for antimicrobial application. This study aimed to evaluate the antibacterial activity of the native Gracilaria fisheri sulfated galactans (NSG) and depolymerized fractions against the marine pathogenic bacteria Vibrio parahaemolyticus and Vibrio harveyi. NSG was hydrolyzed in different concentrations of H2O2 to generate sulfated galactans degraded fractions (SGF). The molecular weight, structural characteristics, and physicochemical parameters of both NSG and SGF were determined. The results revealed that the high molecular weight NSG (228.33 kDa) was significantly degraded to SGFs of 115.76, 3.79, and 3.19 kDa by hydrolysis with 0.4, 2, and 10% H2O2, respectively. The Fourier transformed spectroscopy (FTIR) and 1H− and 13C−Nuclear magnetic resonance (NMR) analyses demonstrated that the polysaccharide chain structure of SGFs was not affected by H2O2 degradation, but alterations were detected at the peak positions of some functional groups. In vitro study showed that SGFs significantly exerted a stronger antibacterial activity against V. parahaemolyticus and V. harveyi than NSG, which might be due to the low molecular weight and higher sulfation properties of SGF. SGF disrupted the bacterial cell membrane, resulting in leakage of intracellular biological components, and subsequently, cell death. Taken together, this study provides a basis for the exploitation and utilization of low-molecular-weight sulfated galactans from G. fisheri to prevent and control the shrimp pathogens.

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Vibrio biofilm inhibitors screened from marine fungi protect shrimp against acute hepatopancreatic necrosis disease (AHPND)

Cited by 18 publications

References 24 publications

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Metal sensing-carbon dots loaded TiO2-nanocomposite for photocatalytic bacterial deactivation and application in aquaculture

Targeting quorum sensing and biofilm formation in the control of Vibrio harveyi infections in Penaeus vannamei

Depolymerized Fractions of Sulfated Galactans Extracted from Gracilaria fisheri and Their Antibacterial Activity against Vibrio parahaemolyticus and Vibrio harveyi

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