Vibrational spectroscopy-based quantification of liver steatosis

Szafraniec, Ewelina; Tott, Szymon; Kuś, Edyta; Augustynska, Dominika; Jasztal, Agnieszka; Filipek, Anna; Chłopicki, Stefan; Barańska, Małgorzata

doi:10.1016/j.bbadis.2019.08.002

Cited by 10 publications

(10 citation statements)

References 35 publications

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“…FTIR spectra of unstained, frozen human liver tissue sections, acquired using a microscope, and attenuated total reflection (ATR)–FTIR spectra of frozen tissue sections deposited on a regular glass slide showed that the average lipids/proteins ratio measured by IR spectroscopy was linearly associated with the concentration of TAGs measured by gas chromatography–MS . ATR–FTIR was also recently used to quantify the degree of steatosis in freeze-dried liver tissue of a mouse model using the histological evaluation of liver sections as reference …”

Section: Introductionmentioning

confidence: 99%

“…FTIR spectra of unstained, frozen human liver tissue sections, acquired using a microscope, and attenuated total reflection (ATR)−FTIR spectra of frozen tissue sections deposited on a regular glass slide showed that the average lipids/proteins ratio measured by IR spectroscopy was linearly associated with the concentration of TAGs measured by gas chromatography− MS. 10 ATR−FTIR was also recently used to quantify the degree of steatosis in freeze-dried liver tissue of a mouse model using the histological evaluation of liver sections as reference. 14 This study was undertaken as a proof of concept to evidence the potential of ATR−FTIR spectroscopy for rapid determination of the grade of liver steatosis and the lipid composition of human liver samples. Using reference information obtained from expert pathologists, total TAG concentrations, and LC− MS lipidomic profiles, our results demonstrate an excellent performance of dry film ATR−FTIR for a direct quantification of the TAG content in liver biopsies, offering information to estimate relative lipid and phospholipid contents, and for the determination of the grade of liver steatosis.…”

Section: ■ Introductionmentioning

confidence: 99%

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Toward Rapid Screening of Liver Grafts at the Operating Room Using Mid-infrared Spectroscopy

Pérez-Guaita

Moreno-Torres²,

Jover

et al. 2020

Anal. Chem.

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The estimation of steatosis in a liver graft is mandatory prior to liver transplantation, as the risk of graft failure increases with the level of infiltrated fat. However, the assessment of liver steatosis before transplantation is typically based on a qualitative or semiquantitative characterization by visual inspection and palpation and histological analysis. Thus, there is an unmet need for transplantation surgeons to have access to a diagnostic tool enabling an in situ fast classification of grafts prior to extraction. In this study, we have assessed an attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopic method compatible with the requirements of an operation room for the evaluation of the lipid contents in human livers. A set of 20 human liver biopsies obtained from organs intended for transplantation were analyzed by expert pathologists, ATR–FTIR spectroscopy, lipid biochemical analysis, and UPLC–ESI(+/−)TOFMS for lipidomic profiling. Comparative analysis of multisource data showed strong correlations between ATR–FTIR, clinical, and lipidomic information. Results show that ATR–FTIR captures a global picture of the lipid composition of the liver, along with information for the quantification of the triradylglycerol content in liver biopsies. Although the methodology performance needs to be further validated, results support the applicability of ATR–FTIR for the in situ determination of the grade of liver steatosis at the operation room as a fast, quantitative method, as an alternative to the qualitative and subjective pathological examination.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Toward Rapid Screening of Liver Grafts at the Operating Room Using Mid-infrared Spectroscopy

Pérez-Guaita

Moreno-Torres²,

Jover

et al. 2020

Anal. Chem.

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“…1E , orange trace), which corresponds to ester groups ((-C = O, -COOH) and is a well-known band used for conventional FTIR-based diagnosis of steatosis. 20,21 The observed intensity changes may indicate different levels of lipidation 19 or lipid oxidation 22 and require complementary techniques for interpretation. Furthermore, we noticed elevations 1452 - 1396 cm -1 that can be assigned to in methylene groups of in proteins and lipids characteristic for salivary gland 23 .…”

Section: Resultsmentioning

confidence: 99%

Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems

Gvazava

Konings

Cepeda-Prado

et al. 2023

Preprint

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Label-free chemical and structural imaging of complex living tissue and biological systems is the holy grail of biomedical research and clinical diagnostics. The current analysis techniques are time-consuming and/or require extensive sample preparation, often due to the presence of interfering molecules such as water, making them unsuitable for the analysis of such systems. Here, we demonstrate a proof-of-principle study using label-free optical photothermal mid-infrared microspectroscopy (O-PTIR) for fast, direct spatiotemporal chemical analysis of complex living biological systems at submicron resolution. While other analytical methods can provide only static snapshots of molecular structures, our O-PTIR approach enables time-resolved and in situ investigation of chemical and structural changes of diverse biomolecules in their native conditions. This comprises a technological breakthrough in infrared spectroscopy to analyze biomolecules under native conditions over time: in fresh unprocessed biopsies, living brain tissue, and vertebrates without compromising their viability.One-Sentence SummaryProof-of-principle application of non-destructive O-PTIR for high-resolution spatiotemporal chemical and structural analysis of unprocessed biopsies, living brain tissue, and vertebrates.

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Section: Methodsmentioning

confidence: 99%

“…In the present results, we quantitatively differentiated NAFLD biochemical status by using Raman signals generated from the microchemical environment of the rat livers. Reports on NAFLD tissue analysis by Raman spectroscopy have increased in recent years [10,[39][40][41][42][43][44][45][46][47]. Kochan et al [48] studied two NAFLD models induced by a HFD (60 kcal %) and a low-carbohydrate high-protein diet by Raman spectroscopy and observed a significant decrease in lipid unsaturation in NAFLD.…”

Section: Diet-based Histological Subgroupsmentioning

confidence: 99%

Raman imaging of rat nonalcoholic fatty liver tissues reveals distinct biomolecular states

et al. 2023

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An essential challenge in diagnosing states of nonalcoholic fatty liver disease (NAFLD) is the early prediction of progression from nonalcoholic fatty liver (NAFL) to nonalcoholic steatohepatitis (NASH) before the disease progresses. Histological diagnoses of NAFLD rely on the appearance of anomalous tissue morphologies, and it is difficult to segment the biomolecular environment of the tissue through a conventional histopathological approach. Here, we show that hyperspectral Raman imaging provides diagnostic information on NAFLD in rats, as spectral changes among disease states can be detected before histological characteristics emerge. Our results demonstrate that Raman imaging of NAFLD can be a useful tool for histopathologists, offering biomolecular distinctions among tissue states that cannot be observed through standard histopathological means.

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Vibrational spectroscopy-based quantification of liver steatosis

Cited by 10 publications

References 35 publications

Toward Rapid Screening of Liver Grafts at the Operating Room Using Mid-infrared Spectroscopy

Toward Rapid Screening of Liver Grafts at the Operating Room Using Mid-infrared Spectroscopy

Label-free high-resolution infrared spectroscopy for spatiotemporal analysis of complex living systems

Raman imaging of rat nonalcoholic fatty liver tissues reveals distinct biomolecular states

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