Vibrational and AFM studies of adsorption of glycine on DLC and silicon-doped DLC

Byrne

Diamond and Related Materials

2015

Diamond-like carbon (DLC) coatings are useful for creating biocompatible surfaces for medical implants. DLC and silicon doped DLC have been synthesised using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of human serum albumin (HSA) with doped and undoped DLC films have been investigated using a range of surface analysis techniques using Raman spectroscopy and atomic force microscopy (AFM). Raman spectra of doped DLC show that silicon doped DLC reduces the growth range of the ID/IG ratio, with a significant red-shift of the G peak position. Following exposure to protein, for undoped DLC the peaks at 1664 cm- 1 and around 1241 cm- 1 can be attributed to amide I and III, respectively, with an increase in the surface morphology of the surfaces giving some indication of the protein structure on the surfaces. Results indicate that HSA exhibit the majority of Î²-sheet during the adsorption on the surfaces. The results showed that the silicon incorporation DLC tends to increase of surface roughness and the adsorbed level of HSA is higher with higher levels of silicon doping of the DLC. Therefore, doping DLC may provide a method of controlling the adsorption of protein. Â© 2015 Elsevier B.V. All rights reserved

Section: Atomic Force Microscopy (Afm) Analysissupporting

confidence: 93%

Characteristic of silicon doped diamond like carbon thin films on surface properties and human serum albumin adsorption

Byrne

Diamond and Related Materials

2015

“…The results of the XPS analysis of the O1s region of the Si-DLC samples showed that the oxygen concentration in the films increases with increasing silicon flow rate, ( Table 2). The obtained results are in good agreement with those provided previously determined values [11]. On the other hand, the band related to silicon atom (Si2p) appeared at around 101 eV.…”

Section: Surface Characterisation Of Prepared Samplessupporting

confidence: 92%

“…Comparative studies improved doped DLC possesses better surface biocompatibility than the undoped counterpart [8]. Thus, to enhance the biocompatibility of DLC, the incorporation of third element dopants like nitrogen [9], fluorine [10], silicon [11] and titanium [12], may be necessary.…”

Section: Introductionmentioning

confidence: 99%

Study of Human Serum Albumin Adsorption and Conformational Change on DLC and Silicon Doped DLC Using XPS and FTIR Spectroscopy

Ahmed¹,

Byrne²,

McLaughlin³

et al. 2013

JBNB

Diamond-like carbon (DLC) coatings are extremely useful for creating biocompatible surfaces on medical implants. DLC and silicon doped DLC synthesised on silicon wafer substrate by using plasma enhanced chemical vapour deposition (PECVD). The effects of surface morphology on the interaction of HSA with doped and undoped DLC films have been investigated. The chemical composition of the surface before and after adsorption was analysed using X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR). Results showed that silicon incorporation DLC tends to increase of sp³/sp² hybridization ratio by decreasing sp² hybridized carbon bonding configurations. Following exposure to solutions containing (0.250 μg/ml) HSA, the results indicated that significant changes in the C, N and O levels on the surfaces with reducing of the Si2p band at 100 eV. From FTIR spectrum, the peaks occur the following functional groups were assigned as amide I and II groups at 1650 cm^-¹ and 1580 cm^-¹. Both XPS and FTIR spectroscopy confirm that HSA was bound onto the surfaces of the DLC and Si-DLC films via interaction of ionized carboxyl groups and the amino group did not play a significant role in the adsorption of protein. These results from peak intensity show that an adsorbed layer of HSA is higher at high level (19%) silicon doping. Therefore doping of DLC may provide an approach to controlling the protein adsorption.<...

“…5(b), the C 1s peak is tted by two peaks at about 283.97 and 284.79 eV, which are attributed to the Si-C and sp 2 C-C bonds, respectively. 27,28 For the O 1s peak, it is decomposed into two peaks at about 531.44 and 532.46 eV, respectively. The peak at $531.44 eV is related to the C]O bond while the peak at $532.46 eV is associated with the C-O-H and/or C-O-C bonds.…”

Section: Resultsmentioning

confidence: 99%

Multiband photoluminescence from carbon nanoflakes synthesized by hot filament CVD: towards solid-state white light sources

et al. 2014