Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue

Abstract

Recently, vibration training is considered as a novel strategy of weight loss; however, its mechanisms are still unclear. In this study, normal or high-fat diet-induced rats were trained by whole body vibration for 8 weeks. We observed that the body weight and fat metabolism index, blood glucose, triglyceride, cholesterol, and free fatty acid in obesity rats decreased significantly compared with nonvibration group (n = 6). Although intrascapular BAT weight did not change significantly, vibration enhanced ATP reduction and increased protein level of the key molecule of brown adipose tissue (BAT), PGC-1α, and UCP1 in BAT. Interestingly, the adipocytes in retroperitoneal white adipose tissue (WAT) became smaller due to vibration exercise and had higher protein level of the key molecule of brown adipose tissue (BAT), PGC-1α, and UCP1 and inflammatory relative proteins, IL-6 and TNFα. Simultaneously, ATP content and PPARγ protein level in WAT became less in rats compared with nonvibration group. The results indicated that vibration training changed lipid metabolism in rats and promoted brown fat-like change in white adipose tissues through triggering BAT associated gene expression, inflammatory reflect, and reducing energy reserve.

Opposite Effects of Voluntary Physical Exercise on β3-Adrenergic Receptors in the White and Brown Adipose Tissue

“…We succeeded to detect UCP-1 mRNA levels by using a highly sensitive amplification master mix. The present results on no changes in UCP-1 mRNA levels in white adipose tissues are consistent with no changes observed in retroperitoneal fat pads in rats exposed to vibration training and fed normal diet [46]. Voluntary exercise used in the present studies failed to influence β3-adrenergic receptor mRNA concentrations in the left heart ventricle.…”

Effects of Coriandrum sativum L. in Association with Physical Exercise in Alloxan-Induced Type 1 Diabetes Mellitus in Rats

“…Chen et al [36] reported that body mass in WBV exercise mice did not change significantly during a six-week experiment period compared to sedentary controls and WBV + dehydroepiandrosterone (DHEA) treated mice. In addition, Sun et al [37] reported no significant differences in body mass between controls and WBV rats subjected to either a high-fat diet or normal diet. Lin et al [38] also showed no differences in body mass of middle-aged mice with or without a four-week WBV exercise regimen.…”

“…It is likely that differences in results can be attributed to different vibration devices (side-alternating system versus linear motions) and specific vibration variables used (e.g., frequency and acceleration). Erceg et al [35] also demonstrated no significant differences for bone biomarkers in a 10-week WBV exercise (30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) program in overweight Latino boys.…”

Tetramethylpyrazine (TMP) switches energy signalling from the PKCζ-GLUT4-glucose pathway back to the SIRT1-CD36-fatty acid pathway similar to resveratrol to ameliorate cardiac myocyte lipotoxicity

“…Additionally, PGC-1α regulates the activity of several transcriptional factors (e.g., PPARγ (Puigserver et al, 1998) and PPARα (Vega, Huss, & Kelly, 2000)). The regulation of transcriptional activity by PGC-1α modulates many metabolic genes involved in both glucose (Kim, You, Ham, Yang, & Yoon, 2015) and fatty acid oxidation (Sun et al, 2015). Several anti-obesity and antidiabetic compounds, such as resveratrol, were reported to increase PGC-1α expression (Svensson et al, 2015).…”