Vibration enhances PGE<sub>2</sub>, IL‐6, and IL‐8 expression in compressed hPDL cells via cyclooxygenase pathway

Phusuntornsakul, Pussadee; Jitpukdeebodintra, Suwanna; Pavasant, Prasit; Leethanakul, Chidchanok

doi:10.1002/jper.17-0653

Cited by 23 publications

(24 citation statements)

References 37 publications

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“…Our previous studies demonstrated LMHF vibration upregulated COX-2, PGE2, IL-6, IL-8 and RANKL, and further increased the levels of these osteoclastogenic factors in compressed PDL cells. 10,11 In addition, we found that the cyclooxygenase pathway is required to induce IL-6 and IL-8 in compressed PDL cells either compressive stress alone or in combination with vibration. On the other hand, the expression of IL-6 and IL-8 in response to vibration alone is the cyclooxygenase pathway independent.…”

Section: Discussionmentioning

confidence: 80%

“…This concentration was previously reported as an effective dose. 6,11 Samples were collected for analysis immediately after the experiments ended. The expression of RANKL and OPG were analysed using quantitative real-time polymerase chain reaction (qPCR).…”

Section: Application Of Mechanical Stress and Cox-2 Inhibitormentioning

confidence: 99%

“…5,6 Our previous studies demonstrated that LMHF vibration upregulates COX-2, PGE2, interleukin (IL)-6, IL-8 and RANKL, and further increases the levels of these osteoclastogenic factors in compressed PDL cells. 10,11 However, the precise mechanisms that PDL cells respond to vibration on RANKL/OPG expression have not yet been determined. We proposed that the expression of RANKL in response to vibration may depend on the cyclooxygenase pathway.…”

Section: Introductionmentioning

confidence: 99%

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Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Benjakul

Leethanakul

Jitpukdeebodintra

2019

Journal of Oral Biology and Craniofacial Research

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This study aimed to examine the effects of PGE2 on RANKL expression in response to vibration and vibration in combination with compressive stress and characterise this transduction pathway in periodontal ligament (PDL) cells. Methods: Cultured human PDL cells obtained from extracted premolar teeth (from six individuals) were subjected to three cycles of vibration (0.3 g, 30 Hz for 20 min every 24 h; V), compressive stress (1.5 g/cm 2 , 48 h; C) or vibration in combination with compressive stress (VC). To investigate whether the expression of RANKL and PGE2 was COX-dependent, PDL cells were treated with indomethacin prior to the onset of mechanical stimulation. RANKL and OPG expressions were examined by quantitative real-time polymerase chain reaction (qPCR). Quantification of PGE2, soluble RANKL (sRANKL) and OPG productions were measured using enzyme-linked immunosorbent assay (ELISAs). Results: All mechanical stresses (V, C and VC) significantly increased PGE2 and RANKL. OPG was not affected by vibration, but was downregulated in compressed cells (C and VC). Indomethacin abolished induction of RANKL and downregulated OPG in response to all mechanical stresses. Conclusion: These results suggest that vibration, compressive stress and vibration in combination with compressive stress induce RANKL expression in human PDL cells by activating the cyclooxygenase pathway.

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Section: Discussionmentioning

confidence: 80%

Section: Application Of Mechanical Stress and Cox-2 Inhibitormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Benjakul

Leethanakul

Jitpukdeebodintra

2019

Journal of Oral Biology and Craniofacial Research

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“…Vibratory stimulus has been proposed as an approach to accelerate tooth movement . In vitro studies suggest vibration may accelerate tooth movement by inducing inflammatory mediators, though animal studies have reported conflicting results and vibration did not accelerate irregularity index improvements in clinical studies . Nevertheless, the ability of vibration to increase the distance of tooth movement is controversial .…”

Section: Introductionmentioning

confidence: 99%

Effects of two frequencies of vibration on the maxillary canine distalization rate and RANKL and OPG secretion: A randomized controlled trial

Siriphan

Leethanakul

Thongudomporn

2019

Orthod Craniofacial Res

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Structured Abstract Objectives To investigate the effects of 30 and 60 Hz vibratory stimulus on canine distalization and RANKL and OPG secretion. Setting and Sample Population Sixty patients requiring canine distalization at the Orthodontic Clinic, Prince of Songkla University. Materials and Methods Patients were randomly assigned to 30 Hz vibration (n = 20), 60 Hz vibration (n = 20), or the control group (n = 20). Modified electric toothbrushes were used to apply vibration to the randomly selected canine for 20 min/day by the investigator combined with 60 cN continuous distalization force from day 1 to day 7. RANKL and OPG were analysed before (T1) and 24 hours (T2), 48 hours (T3) and 7 days (T4) after initiation of distalization. From day 8, vibratory devices were used by the subjects at home. Rate of canine distalization (T1 to 3 months after initiation [T5]) was calculated. Kruskal‐Wallis tests were used for multiple comparisons (significance level, 0.05). Results Canine distalization rate was not different between groups (median; 0.82, 0.87, and 0.83 mm/month for 30, 60 Hz, and control group, respectively; P > 0.05). No within‐ or between‐group differences in RANKL and OPG were observed (P > 0.05), except RANKL on the compression side of the control group was significantly higher at T2, T3 and T4 than T1 (P < 0.001). Conclusion In the clinic, 30 and 60 Hz vibratory stimulus have no additive effect on rate of canine distalization rate, RANKL and OPG secretion or RANKL/OPG ratio.

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“…www.nature.com/scientificreports www.nature.com/scientificreports/ periodontal tissue injury [11][12][13] . Thus, methods to control the inflammatory response and to eliminate periodontal bacteria are considered to be important for the inhibition of periodontal disease.…”

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confidence: 99%

Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha

Nomura

Inaba

Yasuda³

et al. 2020

Sci Rep

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Porphyromonas gulae is a major periodontal pathogen in dogs, which can be transmitted to their owners. A major virulence factor of P. gulae consists of a 41-kDa filamentous appendage (FimA) on the cell surface, which is classified into three genotypes: A, B, and C. Thus far, inhibition of periodontal disease in dogs remains difficult. The present study assessed the inhibitory effects of a combination of clindamycin and interferon alpha (IFN-α) formulation against P. gulae and periodontal disease. Growth of P. gulae was significantly inhibited by clindamycin; this inhibition had a greater effect on type C P. gulae than on type A and B isolates. In contrast, the IFN-α formulation inhibited the expression of IL-1β and COX-2 elicited by type A and B isolates, but not that elicited by type C isolates. Furthermore, periodontal recovery was promoted by the administration of both clindamycin and ifn-α formulation to dogs undergoing periodontal treatment; moreover, this combined treatment reduced the number of fimA genotypes in oral specimens from treated dogs. these results suggest that a combination of clindamycin and ifn-α formulation inhibit P. gulae virulence and thus may be effective for the prevention of periodontal disease induced by P. gulae.

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Vibration enhances PGE₂, IL‐6, and IL‐8 expression in compressed hPDL cells via cyclooxygenase pathway

Abstract: Mechanical vibration may enhance alveolar bone resorption at the compression side during orthodontic tooth movement via a mechanism involving the cyclooxygenase pathway.

Cited by 23 publications

References 37 publications

Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Effects of two frequencies of vibration on the maxillary canine distalization rate and RANKL and OPG secretion: A randomized controlled trial

Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha

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Vibration enhances PGE2, IL‐6, and IL‐8 expression in compressed hPDL cells via cyclooxygenase pathway

Abstract: Mechanical vibration may enhance alveolar bone resorption at the compression side during orthodontic tooth movement via a mechanism involving the cyclooxygenase pathway.

Cited by 23 publications

References 37 publications

Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Low magnitude high frequency vibration induces RANKL via cyclooxygenase pathway in human periodontal ligament cells in vitro

Effects of two frequencies of vibration on the maxillary canine distalization rate and RANKL and OPG secretion: A randomized controlled trial

Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha

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Vibration enhances PGE₂, IL‐6, and IL‐8 expression in compressed hPDL cells via cyclooxygenase pathway