Structured Abstract Objectives To investigate the effects of 30 and 60 Hz vibratory stimulus on canine distalization and RANKL and OPG secretion. Setting and Sample Population Sixty patients requiring canine distalization at the Orthodontic Clinic, Prince of Songkla University. Materials and Methods Patients were randomly assigned to 30 Hz vibration (n = 20), 60 Hz vibration (n = 20), or the control group (n = 20). Modified electric toothbrushes were used to apply vibration to the randomly selected canine for 20 min/day by the investigator combined with 60 cN continuous distalization force from day 1 to day 7. RANKL and OPG were analysed before (T1) and 24 hours (T2), 48 hours (T3) and 7 days (T4) after initiation of distalization. From day 8, vibratory devices were used by the subjects at home. Rate of canine distalization (T1 to 3 months after initiation [T5]) was calculated. Kruskal‐Wallis tests were used for multiple comparisons (significance level, 0.05). Results Canine distalization rate was not different between groups (median; 0.82, 0.87, and 0.83 mm/month for 30, 60 Hz, and control group, respectively; P > 0.05). No within‐ or between‐group differences in RANKL and OPG were observed (P > 0.05), except RANKL on the compression side of the control group was significantly higher at T2, T3 and T4 than T1 (P < 0.001). Conclusion In the clinic, 30 and 60 Hz vibratory stimulus have no additive effect on rate of canine distalization rate, RANKL and OPG secretion or RANKL/OPG ratio.