Viability study of a multiplex diagnostic platform for Chagas disease

Abstract

A new multiplex assay platform was evaluated to detect

Evaluation of In-House ELISA Using Trypanosoma cruzi Lysate and Recombinant Antigens for Diagnosis of Chagas Disease and Discrimination of Its Clinical Forms

“…21 Until now, an effective assay for predicting the clinical evolution of a chronic infection is lacking. 15,22,23 Accordingly, in this study, we evaluated the diagnostic potential of different T. cruzi antigens, namely peptides P013, R13, JL18, JL19, and P0β by ELISA, 21 and investigated the potential use of *Address correspondence to Karina A. Gó mez, Vuelta de Obligado 2490, Buenos Aires 1428. E-mail: drkagomez@gmail.com †Deceased.…”

“…Ongoing studies have focused on endothelin 1, tumor necrosis factor-α, B-type natriuretic peptide, angiotensin-converting enzyme, and also autoantibodies as candidates for disease prognosis. 23,[25][26][27] Among the last ones, cross-reactive Abs against β1-adrenergic and M2 muscarinic receptors have been associated with different arrhythmogenic anomalies, which may contribute to the distinct cardiac alterations observed in patients with SCC. 28 Although receptor Abs are the result of molecular mimicry with parasite ribosomal P proteins, 29,30 R13, P013, and P0β peptides were not good candidates as serological markers of Chagas disease in this study.…”

Advances in Serological Diagnosis of Chagas' Disease by Using Recombinant Proteins

“…Moncayo & Luquetti, 1990;Peralta et al, 1994) Consequently, most of these proteins have been evaluated not only alone and independently from others, but also together as part of mixtures or as fusion proteins, carrying several recombinant epitopes. (Umezawa et al, 1999;Umezawa et al, 2004;Camussone et al, 2009;Foti et al, 2009) Accordingly, a multicenter study evaluating 6 recombinant proteins separately with a serum panel composed by sera from patients of several countries, described that using the set of results of the 6 proteins together had yield a sensitivity and specificity compatible with the reference assays. (Umezawa et al, 1999) Later, the same group evaluated the mixture of the 6 proteins, supporting the use of the mixture to reach the same sensitivity and specificity.…”

Delivering on Promises? The Impact of Kinetoplastid Genomics on Sleeping Sickness, Chagas Disease and Leishmaniasis

“…51 The gap between molecular and serological diagnostics is also bridged by bead-based flow-cytometric technology which utilizes a variety of probing molecules, including antibodies, antigens (native or recombinant) and oligonucleotides, that are covalently bound to paramagnetic carboxylated microspheres. 52,53 Since each probe can be linked to a bead of distinct color, the technology allows simultaneous (multiplex) detection to up to 500 molecules in a single test and consequently diagnosis, typing, subtyping and even assessment of antigenic diversity or drug resistance markers in a single reaction. An initial study compared this technology favorably with ELISA in terms of sensitivity and specificity when recombinant CRA (cytoplasmic repetitive antigen), FRA (flagellar repetitive antigen) and whole T. cruzi cell lysate was used to screen two distinct panels of chagasic patient's sera.…”