Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue

Tsuribe, Patrícia Miyuki; Gobbo, Carlos Alberto Monte; Landim-Alvarenga, F. C.

doi:10.1016/j.fertnstert.2008.03.031

Cited by 13 publications

(8 citation statements)

References 24 publications

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“…; Tsuribe et al. ) using pre‐antral follicles, the healthy follicles analysed from fresh goat ovarian tissue were 72% and 77%, which was considerably higher as compared with our results (59.5% and 40.5% from fresh and vitrified tissues, respectively). However, it was similar to Paynter et al.…”

Section: Discussioncontrasting

confidence: 79%

“…Besides follicular atresia affects all stages of follicular development, these small antral follicles found to be prone to atresia rather than pre-antral and pre-ovulatory stages (Hirshfield 1991;Gosden and Spears 1997), which can explain the reason of having low yield of follicles with normal morphology in fresh and vitrified tissue groups. In similar studies developed by Tsuribe et al (2009) and Rodrigues et al (2004) (Rodrigues et al 2004;Tsuribe et al 2009) using pre-antral follicles, the healthy follicles analysed from fresh goat ovarian tissue were 72% and 77%, which was considerably higher as compared with our results (59.5% and 40.5% from fresh and vitrified tissues, respectively). However, it was similar to Paynter et al (1999) observations, who found only 50% of cattle pre-antral follicles from fresh ovarian tissue with normal morphology.…”

Section: Discussionsupporting

confidence: 66%

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Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

Faheem

Carvalhais

Baron

et al. 2013

Reprod Domestic Animals

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This study was performed to evaluate the structural preservation of antral follicles after bovine ovarian tissue vitrification using histological analysis. Ovaries (n = 30) of slaughtered cows were cut into small fragments using a scalpel blade, and the ovarian tissues were randomly assigned to vitrification using 15% dimethyl sulphoxide (DMSO) and 15% ethylene glycol (EG) and fresh tissues (control) groups. For histological evaluations, fresh and post-thawing ovarian tissues were immediately fixed, serially sectioned into 5-μm sections and stained with haematoxylin and eosin (H&E). Nine serial sections per fragment were subjected for morphological assessment. The diameter of the antral follicles was determined and classified into four groups: 1 (≤1 mm), 2 (>1-2 mm), 3 (>2-3 mm) and 4 (>3-4 mm). Then, follicular morphology was evaluated in relation to atresia and categorized into seven grades: Grade A (healthy follicle); Grades B, C and D (early atresia); Grades E and F (moderate atresia); and Grade G (advanced atresia). The results revealed that small diameters of antral follicles (1 and 2 mm) were more susceptible for cryoinjury. The normal follicular morphology (Grade A) was not affected by vitrification throughout follicle diameters. Nevertheless, some damage features were monitored after vitrification. In conclusion, the morphological structure of bovine antral follicles could be successfully preserved by ovarian tissue vitrification.

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Section: Discussioncontrasting

confidence: 79%

Section: Discussionsupporting

confidence: 66%

Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

Faheem

Carvalhais

Baron

et al. 2013

Reprod Domestic Animals

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“…This evaluation method might have skewed the viable follicles to a smaller number as determined by the fluorescent staining method compared to NR staining. To the best of our knowledge only a few studies on cryopreservation of ovarian tissue have been carried out culturing tissue strips in vitro for more than 24 h after thawing to evaluate the survival and development of follicles [25,44,61,65,66]. Finally, results of the present study showed that stainless steel mesh can be used as a carrier device in the vitrification procedure.…”

Section: Discussionmentioning

confidence: 72%

In vitro development of human primordial follicles to preantral stage after vitrification

Khosravi

Reid

Moini

et al. 2013

J Assist Reprod Genet

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Purpose The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue. Methods Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed. Results High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P <0.05) whereas primordial and transitory follicles showed a significant decrease (P <0.05) compared to their respective counterparts at day 0 of culture.Conclusions Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.

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“…It has been reported that exposure of follicles to cryoprotectant agents could compromise their rates of survival (Rodrigues et al., ,b), even without freezing (Tsuribe, Gobbo, & da Cruz Landim‐Alvarenga, ). To improve vitrification, some modifications have been applied by researchers, which were the changes in the concentration and type of cryoprotectant (Herraiz et al., ; Lunardi et al., ; Yamaji et al., ), time (Ting, Yeoman, Lawson, & Zelinski, ), temperature (Mouttham & Comizzoli, ), the device used (Herraiz et al., ; Lunardi et al., ) and tissue size (Lu et al., ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Mohammadzadeh

Safdarian

Amidi

et al. 2017

Reprod Domestic Animals

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The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS. Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture (p < .05 vs. Fresh). Following two weeks of culture, all groups revealed a considerable fall in the number of viable follicles (p < .05 vs. Fresh). There was an increase in DNA fragmentation of connective tissue cells but not in the follicles (p < .05). Our results showed the better application of αMEM supplemented with BSA as a vitrification solution in improvement of cryopreservation effects and maintenance of follicular survival.

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Viability of primordial follicles derived from cryopreserved ovine ovarian cortex tissue

Cited by 13 publications

References 24 publications

Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

In vitro development of human primordial follicles to preantral stage after vitrification

Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

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