Viability of Porcine Embryos after Thawing at Various Temperatures with Freezing Slowly

Iwasaki, Tadaaki; Kojima, Toshiyuki; Zeniya, Yumie; Totsukawa, Kiyoshi

doi:10.2508/chikusan.66.705

Cited by 3 publications

(7 citation statements)

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“…A reliable method of evaluating embryo quality is to assess the number of cells, but in porcine embryos this procedure requires fixation and staining, which produce unviable embryos. We did not evaluate the relationship between diam et er measurement (or morphological assessment) and embryo quality (number of cells) because Iwasaki et al [11] already reported that these two parameters are positively correlated. Establishment of embryo quality assessment by measurement of cell numbers without fixation (by live staining) may be advantageous in the field of embryo collection and transfer.…”

Section: Discussionmentioning

confidence: 99%

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Relationship between Time Elapsed after Human Chorionic Gonadotropin Administration and Developmental Stage in Porcine Embryos Collected from Prepubertal Gilts

Fujino¹,

Nakamura²,

Kobayashi³

et al. 2006

Journal of Reproduction and Development

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Abstract. We examined the relationship between the time elapsed after human chorionic gonadotropin (hCG) administration and developmental stage of porcine embryos after collection. Prepubertal gilts, 7 to 8 months old, were given 1500 IU equine chorionic gonadotropin (eCG) intramuscularly, followed by 500 IU hCG 72 h later. The treated gilts were inseminated artificially on Day 1 (Day 0=the day of hCG administration) and on Day 2. Embryos were collected surgically on Day 6 (140, 144, and 147 h after hCG administration) or on Day 7 (164, 168, and 171 h), and the developmental stages of the collected embryos were examined. From 75.2% (276/367) of the prepubertal gilts treated with hormones, we collected an average of 20.7 embryos per gilt with normal morphology. At 140 h after hCG administration, morulae (54.4%) could be collected. At 144 h, morulae and early blastocysts (57.7% and 28.9%, respectively) were collected. By 147 h, the proportion of embryos at the blastocyst to expanded blastocyst stages had increased (10.0%). From 164 h to 171 h, expanding or expanded blastocysts of more than 200 µm in diameter and hatched blastocysts could be collected. The proportion of hatched blastocysts increased from 3.2% (164 h) to 41.0% (171 h). These results suggests that although the number of ovulations differed among gilts, porcine embryos at the appropriate stages can be collected efficiently by controlling the time elapsed between hCG administration and embryo collection.

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Section: Discussionmentioning

confidence: 99%

“…Measurement of embryo diameter is a reliable way of estimating developmental stage [10][11][12], but the precise relationship between length of time after treatment with hormone(s) and embryo diameter has not yet been investigated.…”

mentioning

confidence: 99%

Relationship between Time Elapsed after Human Chorionic Gonadotropin Administration and Developmental Stage in Porcine Embryos Collected from Prepubertal Gilts

Fujino¹,

Nakamura²,

Kobayashi³

et al. 2006

Journal of Reproduction and Development

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“…This assessment was used in our previous report [7]. Although it is important to confirm development during in vitro culture, the development may be influenced by culture conditions used.…”

Section: Discussionmentioning

confidence: 99%

“…If younger-stage embryos were recovered, the embryos were obliged to incubate in vitro until the embryos developed to expanded blastocysts. However, the in vitro incubation, of which period is varied depending on the initial stage, adversely affects the freezing tolerance as well as the in vivo survival, while the longer the embryos incubate the larger the diameter of the embryos expands and the less the velocity of growth (an increase of number of viable nuclei) advances [7]. They demonstrated that the diameter had a good correlation with the number of viable nuclei, and that the adverse effect of incubation on freezing tolerance was almost negligible if incubation period for Day 6 embryos was limited to less than 15 h [7].…”

Section: Discussionmentioning

confidence: 99%

“…Egg yolk was prepared by the method described elsewhere [6]. The embryos were directly immersed in 10% (1.8 M) ethylene glycol (Wako Pure Chemicals, Osaka, Japan) solution for 5 min and moved to 10% ethylene glycol solution containing 0.2 M trehalose (Sigma) for 5 min at room temperature (25 C) [7]. During equilibration with the 10% ethylene glycol solution containing 0.2 M trehalose, about 10 embryos were loaded into a 0.25-ml plastic straw (A201, I.M.V., L'Aigle, France).…”

Section: Freezing and Thawingmentioning

confidence: 99%

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Effect of Dietary Administration with Linoleic Acid and .ALPHA.-Tocopherol on Freezing Tolerance in Porcine Embryos.

Kojima¹,

Iwasaki

Zeniya³

et al. 1996

Journal of Reproduction and Development

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A Three-generation Study on Male-mediated Developmental Toxicity in Mice Exposed to Bisphenol A and Bisphenol A–Irradiation Combination

Dobrzyńska,

Gajowik

2024

Male-Mediated Developmental Toxicity

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This chapter summarizes our long-term research on the effects of preconceptional exposure covering a full spermatogenesis cycle of pubescent or adult male mice to BPA or to a combination of BPA and irradiation on the male gametes and the development of two generations of their offspring. There were some differences in the response of male germ cells to bisphenol A exposure between adult and pubescent males. The harmful effect induced in the gametes of pubescent males by bisphenol A was more clear, suggesting the higher susceptibility of germ cells of adolescent mammals. Exposure of males to BPA alone or in combination with irradiation for a full cycle of spermatogenesis may cause heritable changes transferable to subsequent generations, which lead to unsuccessful fertilization or preimplantation losses as well as to death of pups after birth. Such exposure may also diminish the sperm quality of the males of the F1 generation leading to unsuccessful fertilization and induce obesity in the F1 offspring of exposed males. Combined treatment mainly intensified the harmful effect induced by BPA in male germ cells. Transgenerational effects on subsequent generations might involve genetic and epigenetic mechanisms.

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Viability of Porcine Embryos after Thawing at Various Temperatures with Freezing Slowly

Cited by 3 publications

References 0 publications

Relationship between Time Elapsed after Human Chorionic Gonadotropin Administration and Developmental Stage in Porcine Embryos Collected from Prepubertal Gilts

Relationship between Time Elapsed after Human Chorionic Gonadotropin Administration and Developmental Stage in Porcine Embryos Collected from Prepubertal Gilts

Effect of Dietary Administration with Linoleic Acid and .ALPHA.-Tocopherol on Freezing Tolerance in Porcine Embryos.

A Three-generation Study on Male-mediated Developmental Toxicity in Mice Exposed to Bisphenol A and Bisphenol A–Irradiation Combination

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