Viability of one-cell bovine embryos cultured in vitro: comparison of cell-free culture with co-culture

Goto, Keita; Iwai, Naomichi; Ide, Kaori; Takuma, Yoshihiro; Nakanishi, Yoshihiko

doi:10.1530/jrf.0.1000239

Cited by 27 publications

(10 citation statements)

References 4 publications

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“…Therefore, we believe that t h e b l a s t o c y t d e v e l o p m e n t r a t e w a s n o t s u b s t a n t i a l l y i n f l u e n c e d b y t h e m e d i u m replacements on days 5-6 post IVF because the 8-cell block barrier had previously invalidated this procedure. Several methods have been reported for overcoming the 8-cell block , such as co-culture systems with granulosa [19][20][21], oviduct epithelial cells [11,12], trophoblastic tissues [22], addition of growth factors [23][24][25][26][27], glucose deprivation [28], culture under low oxygen concentration [29,30], addition of reducing agents [13], and cytokines [32,33]. Particularly in culture systems that replace coculture with somatic cells, methods employing a conditioned medium of bovine oviduct epithelial cells [11,12], buffalo rat hepatocytes [13], bovine granulosa cells, and vero cells [14] have been documented.…”

Section: Discussionmentioning

confidence: 99%

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Fujita

Umeki

Shimura

et al. 2006

Journal of Reproduction and Development

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Abstract.We investigated the effect of group culture on bovine embryo development, and also investigated the effect of embryo-culture conditioned medium on developmental competence of individually cultured bovine embryos. Slaughterhouse-derived bovine oocytes were matured and fertilized in vitro. The presumptive zygotes were cultured individually or cultured in groups of 2 to 5 embryos with a constant culture density (5 µl/embryo). After 7 days of culture, the rates of embryos developed to the blastocyst stage were significantly higher (P<0.05) in group cultures of more than 3 embryos/drop than for embryo culture of 1 or 2 embryos/drop. These results suggest a beneficial effect of group culture may be exerted by possible growth promoting factors secreted by embryos. In the next experiment, we investigated the effect of timing of fresh medium replacement on the development of embryos cultured in groups. The blastocyst formation rate was lower when culture medium was replaced freshly on days 2-4 after fertilization than on days 5-6. The blastocyst formation rates of single-cultured embryos were significantly (p<0.05) increased by the addition of conditioned medium derived from multiple-embryo culture. These results indicate that group culture promotes embryo development and that embryo culture-derived conditioned medium is effective for supporting development of single cultured embryos. Key words: Bovine, Conditioned medium, Development, Embryo, Single embryo (J. Reprod. Dev. 52: [137][138][139][140][141][142] 2006) ecently, ultrasound-guided ovum pick-up (OPU) has enabled repeat collection of oocytes from live cattle for genetic improvement [1][2][3]. However, the efficiency of embryo production by OPU is still low because of the limited number of oocytes collected and used for in vitro fertilization (IVF) and culture (IVC). In our field data, the blastocyst formation rate decreased when the number of in vitro fertilized oocytes collected from individual heifers was less than 3. Therefore, it is important to increase the efficiency of blastocyst production from small numbers of in vitro fertilized oocytes collected by OPU.Previous studies in mice [4, 5], sheep [6], and cattle [7][8][9] have demonstrated that group cultures promote embryo development compared with single-culture or co-culture with small numbers of embryos. In murine embryos, the blastocyst formation rate is promoted by an increase of embryo density in culture drops [10], suggesting that specific factor(s) are secreted from the embryos to influence embryo growth and development in an autocrine or paracrine manner. In the non-coculture system, embryo development is promoted by adding conditioned medium derived from bovine oviductal epithelial cells [11,12], buffalo/rat hepatocytes [13], bovine granulosa cells, or Vero

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Section: Discussionmentioning

confidence: 99%

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Fujita

Umeki

Shimura

et al. 2006

Journal of Reproduction and Development

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“…Several investigators have eliminated extraneous somatic cells as components of embryo culture [8,14,[19][20][21]; however, they have not replaced serum or BSA in maturation, fertilization, or culture media, and these components contain unknown factors that might contribute beneficial effects [22]. The viability of uterine-stage bovine embryos produced in vitro in coculture-free media has been demonstrated by successful embryo transfers (ET) yielding offspring [18,21,23].…”

Section: Introductionmentioning

confidence: 99%

“…The viability of uterine-stage bovine embryos produced in vitro in coculture-free media has been demonstrated by successful embryo transfers (ET) yielding offspring [18,21,23]. To optimize the utility of IVMFC for research and for commercial purposes, the requirements of such embryos should be known.…”

Section: Introductionmentioning

confidence: 99%

Production of viable bovine blastocysts in defined in vitro conditions

Keskintepe

Burnley²,

Brackett³

1995

Biology of Reproduction

147

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Our purpose was to obtain viable blastocysts via in vitro maturation, fertilization, and culture (IVMFC) in serum- and BSA-free media (defined conditions) and to document viability by pregnancy initiation following embryo transfer (ET). Oocytes were matured in modified TCM 199 (mTCM 199) with 100 micrograms/ml ovine (o)LH, inseminated in TALP- or defined medium (DM)-based media, and cultured up to 9 days in synthetic oviductal fluid (SOF) prepared with 6 mg/ml polyvinyl alcohol (PVA) instead of BSA and buffered with 25 mM HEPES with experimental modifications. Modifications for embryo culture included supplementation with Minimum Essential Medium amino acids (MEM), Minimum Essential Medium nonessential amino acids (NEA), the combination of MEM and NEA, citrate (c; 0.5 mM), glutamine (1 mM), or combinations of these. Proportions of immature oocytes selected for IVM that cleaved (IVF) and that reached the blastocyst stage in SOF were 66.3% and 10.9%, respectively. Supplementation of SOF with citrate and nonessential amino acids (i.e., c-SOF + NEA) enabled 85.1% cleavage and 42.6% blastocyst development of oocytes selected for IVM. In conjunction with IVM in mTCM 199 plus 100 micrograms/ml oLH and IVC in c-SOF + NEA, efforts to eliminate protein from the fertilization medium revealed modified DM (mDM) prepared with PVA instead of BSA to be superior to TALP prepared with PVA; IVMFC data for blastocyst development were 27.4% vs. 18.2% (p < 0.05), respectively. The use of mDM for sperm preparation and IVF yielded comparable blastocyst development when either BSA or PVA was included.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…Total cell counts that were produced in different culture systems varied signifi cantly among embryos at the same stage of development (Pinyopummintr and Bavister, 1991;Ectors et al, 1993;Goto et al, 1994;Rieger et al, 1995). However, total cell counts do not necessarily refl ect the overall integrity and viability of preimplantation IVF embryos.…”

Section: Cattlementioning

confidence: 99%

Embryo Transfer andin vitroFertilization

Hasler¹

2007

Comparative Reproductive Biology

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Viability of one-cell bovine embryos cultured in vitro: comparison of cell-free culture with co-culture

Cited by 27 publications

References 4 publications

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Effect of Group Culture and Embryo-culture Conditioned Medium on Development of Bovine Embryos

Production of viable bovine blastocysts in defined in vitro conditions

Embryo Transfer andin vitroFertilization

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