Embryo Transfer and<i>in vitro</i>Fertilization

Hasler, J.F.

doi:10.1002/9780470390290.ch8

Cited by 6 publications

(5 citation statements)

References 290 publications

(326 reference statements)

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“…When asynchrony is much beyond ± 24 hr, recipient conception rates following ET are reduced [14]. An additional report [13] shows that embryo quality influences pregnancy rates, but the stage of TEs, ranging from late morulae (stage code 4) to expanded blastocysts (stage code 7), did not affect pregnancy rates. Previous reports [8,14] noted a decrease in pregnancy rate with each corresponding decrease in quality score.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, the most frequently reported average number of embryos recovered from multiple ovulations is between four and seven [13]. However, superovulatory response of donor animals is variable and unpredictable [18,35].…”

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confidence: 99%

“…However, superovulatory response of donor animals is variable and unpredictable [18,35]. It is generally accepted that the observed variation stems more from genetic variation among individual females than factors such as the specific gonadotropin used or the breed of cattle [13]. Even so, obtaining more embryos would enable us to increase the chances for successful ET.…”

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confidence: 99%

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Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle

Lee

Song

Lim³

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to investigate the influence of key parameters (donor parity, milk production, post-parturient day, season and milk recording data) associated with efficiency of embryo recovery (ER) in Holstein cattle. Elite Holstein cows and heifers were selected for ER, while Holstein heifers were used as recipients. The numbers of transferable embryos (TEs) produced were not significantly different when analyzed in terms of donor parity, milk production, postparturient day and season. However, the numbers of TEs were significantly increased when the milk protein (%; P)/fat (%; F) ratio was over 0.95 and/or the milk urea nitrogen (MUN) was between 12 and 18 dl/ml. The results from ET showed no differences in pregnancy rates among Holstein heifers receiving other types, developmental stage codes and quality grades of embryos. The mean interval from ER to artificial insemination was 60.6 days. Moreover, 19 offspring that had milk recording data showed a similar milk yield performance to that of the donor cows. In conclusion, this study showed that in Holstein cows, embryos were recovered and transferred and resulted in production of viable calves. Furthermore, P/F ratio and MUN could be candidate indicators for selection of high-efficiency donor cows.KEY WORDS: embryo recovery, embryo transfer, Holstein cattle, milk protein/fat ratio, superovulation.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle

Lee

Song

Lim³

et al. 2012

The Journal of Veterinary Medical Science

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“…In vitro oocyte maturation~IVM! has been highly successful in many mammals including domestic cats, but the efficiency in dogs is low~Luvoni et al, 2006;Rodrigues & Rodrigues, 2006;Hasler, 2007!. Reproductive physiology in the canine differs from most mammals in that oocytes are ovulated at meiotic prophase I~germinal vesicle stage!, and meiosis is completed in the oviduct~Luvoni Reynaud et al, 2006;Rodrigues & Rodrigues, 2006;Songsasen & Wildt, 2007!. Less is understood about canine reproduction, making IVM in dogs difficult. Therefore, it is important to know more about ZP ultrastructure and its interactions with cumulus cells to improve culture conditions for canine IVM.…”

Section: Introductionmentioning

confidence: 99%

Imaging the Zona Pellucida of Canine and Feline Oocytes Using Scanning Electron Microscopy

Lunn

Wright

2009

Microsc Microanal

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Ultrastructure of the zona pellucida (ZP) of canine and feline oocytes has not been fully investigated. The objective of the study was to evaluate the potential use of the low vacuum scanning electron microscope (LVSEM) with oocytes. This required development of a method to prepare canine and feline cumulus-oocyte complexes (COCs) for LVSEM to provide ultrastructural information on the ZP. COCs were collected from ovaries, and cumulus cells were either partially or completely removed to reveal the ZP. COCs and zona-intact oocytes were fixed at 4 degrees C for 1 h in 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.4 and subsequently viewed wet or further processed by critical point drying, and viewed uncoated or sputter coated with gold. Although the spongy surface of the ZP was visible at low vacuum in uncoated oocytes, coated oocytes had more details at high vacuum. The ZP surface of canine and feline oocytes contained numerous various-sized, spherical or elliptical pores that narrowed centripetally splitting into several smaller, deep pores. The round to oblong cumulus cells tightly surrounded the ZP. Each corona radiata cumulus cell tapered into a thin projection that entered the ZP. Our detailed techniques will enable future studies connecting ultrastructural and molecular aspects of oocyte maturation and development in mammals.

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“…The technique of embryo transfer was first implemented in the 1970s and has been developed continually and perfected since that time. For example, in a country like Brazil, by 1995, 40,000 embryos were transferred and by 1999 this figure went up to 74,000, of which 60% were frozen (Thibier, 1997; Hasler, 2007).…”

Section: Introductionmentioning

confidence: 99%

Assessment of the viability of embryos stored in liquid nitrogen produced commercially using culture medium as a complementary test for stereoscopic microscopy

Godínez

Galina

León

et al. 2011

Zygote

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Summary The objective of the present study was to evaluate the viability of frozen embryos obtained from various private farmers in a culture medium for 4 h. Forty-seven embryos were used that had been previously graded as good and fair. These embryos were evaluated using stereoscopic microscopy by experienced clinicians prior to freezing. Embryos were divided in two groups: the non-cultured group, made up of six good quality embryos, and five fair; and the cultured group that consisted of 20 good quality embryos and 16 fair. Fifty-four per cent of the good quality embryos achieved a favourable development during culture whereas just 42% of embryos determined to be fair were observed to have adequate development. This evaluation was undertaken by serial photographs obtained at the onset of culture and 4 h later. This finding was corroborated by a more specific technique: terminal deoxynucleotide transferase dUTP nick end labelling-bromodeoxyuridine (TUNEL-BrdU). These results are indicative of the necessity of tight quality controls for commercially produced frozen embryos, as once thawed it is unlikely that clinicians will examine them to determine their physiological status prior to transfer.

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Embryo Transfer andin vitroFertilization

Cited by 6 publications

References 290 publications

Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle

Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle

Imaging the Zona Pellucida of Canine and Feline Oocytes Using Scanning Electron Microscopy

Assessment of the viability of embryos stored in liquid nitrogen produced commercially using culture medium as a complementary test for stereoscopic microscopy

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