Viability of human periodontal ligament fibroblasts in milk, Hank’s balanced salt solution and coconut water as storage media

Souza, Beatriz Dulcinéia Mendes de; Lückemeyer, Débora Denardin; Reyes-Carmona, Jessie; Felippe, Wilson Tadeu; Simões, Cláudia Maria Oliveira; Felippe, Mara Cristina Santos

doi:10.1111/j.1365-2591.2010.01809.x

Cited by 45 publications

(102 citation statements)

References 24 publications

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“…Milk is a proven storage medium for avulsed teeth (6,8,11), but cell culture studies have shown that its effectiveness decreases over time (6)(7)(8), probably because the drop in its pH creates an unsuitable environment for cell survival (3). Thus, it was hypothesized that the renewal of milk for storing the avulsed tooth could prevent the drop in its pH.…”

Section: Discussionmentioning

confidence: 99%

“…While some demonstrated effectiveness for only up to 3 h (15,19) or 6 h (4), other showed effectiveness for 24 h (6,17,(20)(21)(22) or for up to 48 h (7,8). When culture plates were maintained at 20 °C and 37 °C, milk was the best storage medium for up to 48 and 24 h, respectively (7).…”

Section: Introductionmentioning

confidence: 99%

“…Besides having low bacterial content (3,4), physiologic osmolality (230-270 mOsm/kg) and neutral pH (6.5-6.8), it provides the cells with nutrients (3) and growth factors (12,13). Although there has been some controversy (6,8), it has been suggested that milk with a lower fat content might be more appropriate to maintain PDLF viability than milk with a higher fat content (14). Previous studies indicate that cold milk is more effective than milk at room temperature (3,10,(15)(16)(17).…”

Section: Introductionmentioning

confidence: 99%

“…Milk is recommended as a transport medium for avulsed teeth (3)(4)(5)(6)(7)(8)(9)(10)(11). Besides having low bacterial content (3,4), physiologic osmolality (230-270 mOsm/kg) and neutral pH (6.5-6.8), it provides the cells with nutrients (3) and growth factors (12,13).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

et al. 2017

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The purpose of this study was to evaluate if the renewal of milk as a storage medium, every 12, 24 and 48 h, is able to increase its ability to maintain human periodontal ligament fibroblasts (PDLF) viability over time. PDLF were soaked in Minimum Essential Medium at 37 °C (MEM-37) (positive control), tap water (Water) (negative control) and in skimmed milk (44 wells) at 5 °C and 20 °C. The skimmed milk was renewed every 12 h (Milk-12), 24 h (Milk-24) and 48 h (Milk-48) in 11 wells of each plate, and the milk in the remaining 11 wells of each plate was maintained in situ (not renewed milk) (NRM). After 24, 48, 72, 96 and 120 h, cell viability was determined by the tetrazolium salt-based colorimetric (MTT) assay. Data were statistically analyzed by Kruskal-Wallis, Scheffé and Mann-Whitney tests (α=5%). At 5 °C, only Milk-48 was significantly better than NRM. At 20 °C, NRM was more effective than Milk-12 and Milk-24 in all time periods. In relation to the temperature (5 °C or 20 °C), renewal of milk at 5 °C was better in maintaining cell viability than the renewal at 20 °C. In conclusion, the renewal of milk was able to increase its ability to maintain cell viability only when performed every 48 h in milk maintained at 5 °C.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Milk Renewal on Cell Viability In Vitro at Different Time Frames

et al. 2017

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“…The agents used to neutralize the pH of coconut water must be stable over time and have no negative influence on the osmolarity. Another important factor is the possible difference regarding properties of natural versus industrialized coconut water, which may influence the final results 19 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Soares

Rocha

Reis

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Avaliar a eficácia de quatro tipos de substâncias usadas para ajuste do pH da água de coco (AC) sobre a viabilidade de fibroblastos humanos (HFF). Material e método: O pH da AC natural e industrializada foi ajustado para pH 7,0 utilizando: (1) Hidróxido de Sódio (NaOH), (2) bicarbonato de sódio (NaHCO3), (3) Trietanolamina (C6H15NO3), (4) 2-Amino-2-Methil-1-propanol (C4H11NO). Células HFF foram plaqueadas em 2×10 4 células/poço em placas de 96 poços e mantidas nas diferentes soluções de AC acima durante 2 h e 4 h. Controle positivo foi representado por HFF mantidas em DMEM e o controle negativo por água da torneira. A viabilidade celular foi avaliada pelo método de MTT Formazan. Os dados foram analisados por 3-way ANOVA seguido pelo teste de Tukey e Dunnett. Resultado: A viabilidade celular não é influenciada pelo período de avaliação, e as interações entre AC e período de avaliação, AC e método de ajuste de pH, método de ajuste de pH e período de avaliação (p>0,05). Conclusão: O produto utilizado para ajuste do pH não interfere na viabilidade de FH, embora, haja uma tendência de melhor desempenho em AC natural.Descritores: Avulsão dentária; água de coco; viabilidade celular. AbstractObjective: This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method: Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO 3 ), (3) Triethanolamine (C 6 H 15 NO 3 ), (4) 2-Amino-2-Methil-1-Propanol (C 4 H 11 NO). Fibroblasts were plated at 2×10 4 / well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey's and Dunnet's test. Result: There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion: The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

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