Viability of human fibroblasts in coconut water as a storage medium

Moreira-Neto, José Jeová Siebra; Gondim, Juliana Oliveira; Raddi, Maria Stella Gonçalves; Pansani, Cyneu Aguiar

doi:10.1111/j.1365-2591.2009.01591.x

Cited by 56 publications

(79 citation statements)

References 21 publications

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“…Most studies evaluating CW as a storage medium for avulsed teeth did not investigate the pH neutralization 18,24 . To date, only three studies have been concerned with adjusting the pH to physiological conditions 11,14,15 . However, these studies have not compared the effects of other neutralizer agents, potentially applicable for this purpose, in order to better preserve the CW properties.…”

Section: Discussionmentioning

confidence: 99%

“…The immortalized cell culture reduces the need for using large amounts of replicates for statistical validation of the results. Various in vitro studies evaluating interim storage media have used this experimental model, due to their ability to multiply rapidly and their unlimited lifespan 14,17,24 .…”

Section: Discussionmentioning

confidence: 99%

“…In this sense, various storage media have been investigated for their ability to maintain the viability of PDL cells for as long as possible. These storage media include milk, culture media, Viaspan, Hank's Balanced Salt Solution (HBSS), Gatorade®, propolis 8,9 , soy milk 10,11 , and coconut water (CW) [11][12][13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, some studies [11][12][13][14][15] have proposed CW as a promising storage medium for avulsed teeth. The natural, fresh and tender CW is a sterile solution, with physiological osmolarity used in the past as a blood plasma substitute 16 .…”

Section: Introductionmentioning

confidence: 99%

“…Despite the favorable characteristics of CW, studies involving this solution as a storage medium have shown contradictory results. Some studies report that CW showed better performance than other storage media such as HBSS or milk in terms of maintaining PDL cell viability after avulsion [11][12][13]15,18 ; another study addressed low capability to preserve cells 14 . The pH adjustment of this solution seems to be an indispensable stage for its production at an industrial scale 9 .…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of coconut water neutralized by different agents on the viability of human fibroblasts: an in vitro study

Soares

Rocha

Reis

et al. 2016

Rev. odontol. UNESP

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ResumoObjetivo: Avaliar a eficácia de quatro tipos de substâncias usadas para ajuste do pH da água de coco (AC) sobre a viabilidade de fibroblastos humanos (HFF). Material e método: O pH da AC natural e industrializada foi ajustado para pH 7,0 utilizando: (1) Hidróxido de Sódio (NaOH), (2) bicarbonato de sódio (NaHCO3), (3) Trietanolamina (C6H15NO3), (4) 2-Amino-2-Methil-1-propanol (C4H11NO). Células HFF foram plaqueadas em 2×10 4 células/poço em placas de 96 poços e mantidas nas diferentes soluções de AC acima durante 2 h e 4 h. Controle positivo foi representado por HFF mantidas em DMEM e o controle negativo por água da torneira. A viabilidade celular foi avaliada pelo método de MTT Formazan. Os dados foram analisados por 3-way ANOVA seguido pelo teste de Tukey e Dunnett. Resultado: A viabilidade celular não é influenciada pelo período de avaliação, e as interações entre AC e período de avaliação, AC e método de ajuste de pH, método de ajuste de pH e período de avaliação (p>0,05). Conclusão: O produto utilizado para ajuste do pH não interfere na viabilidade de FH, embora, haja uma tendência de melhor desempenho em AC natural.Descritores: Avulsão dentária; água de coco; viabilidade celular. AbstractObjective: This study evaluated four types of pH adjustment of the coconut water (CW) on viability of human fibroblasts (HFF). Material and method: Natural and industrialized CW were adjusted to pH 7.0 using: (1) Sodium Hidroxide (NaOH), (2) Sodium bicarbonate (NaHCO 3 ), (3) Triethanolamine (C 6 H 15 NO 3 ), (4) 2-Amino-2-Methil-1-Propanol (C 4 H 11 NO). Fibroblasts were plated at 2×10 4 / well in 96 well plates and maintained in the CW solutions for 2 h and 4 h. Positive control was represented by HFF maintained in DMEM and the negative control by tap water. Cell viability was analyzed by MTT formazan method. Data were analyzed by 3-way ANOVA followed by Tukey's and Dunnet's test. Result: There are no significant effect on the cell viability regarding type of CW, period of evaluation, and the interactions between CW and period of evaluation, CW and pH adjustment method, pH adjustment method and period of evaluation (p>0.05). Conclusion: The product used for CW pH adjustment did not influenced HFF viability, thought there are a tendency of better performance in natural CW.

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