Viability of cultured periodontal pocket epithelium cells and <i>Porphyromonas gingivalis</i> association

Dierickx, Kurt; Pauwels, Martine; Eldere, Johan Van; Cassiman, Jean‐Jacques; Steenberghe, Daniël van; Quirynen, Marc

doi:10.1034/j.1600-051x.2002.291103.x

Cited by 14 publications

(9 citation statements)

References 64 publications

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“…Periodontal bacteria were incubated with H400 cells at a concentration of 1 × 10 9 ml (equivalent to approximately 100 bacteria per epithelial cell) for 1 h. This ratio of bacteria (1 : 100, epithelial cell to bacteria) was comparable with previous studies which indicate the maximum number of bacteria associated with epithelial cells in the periodontal pocket, thereby providing clinical relevance. 17 Cells or supernatants were analysed subsequently for NF-κB nuclear translocation by high content analysis, gene expression by sq-RTPCR and IL-8 protein by ELISA.…”

Section: Methodsmentioning

confidence: 99%

Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria

et al. 2012

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Chronic periodontal diseases are characterised by a dysregulated and exaggerated inflammatory/immune response to plaque bacteria. We have demonstrated previously that oral keratinocytes up-regulate key molecular markers of inflammation, including NF-κB and cytokine signalling, when exposed to the periodontal bacteria Porphyromonas gingivalis and Fusobacterium nucleatum in vitro. The purpose of the current study was to investigate whether α-lipoic acid was able to abrogate bacterially-induced pro-inflammatory changes in the H400 oral epithelial cell line. Initial studies indicated that α-lipoic acid supplementation (1-4 mM) significantly reduced cell attachment; lower concentrations (<0.5 mM) enabled >85% cell adhesion at 24 h. While a pro-inflammatory response, demonstrable by NF-κB translocation, gene expression and protein production was evident in H400 cells following exposure to P. gingivalis and F. nucleatum, pre-incubation of cells with 0.5 mM α-lipoic acid modulated this response. α-Lipoic acid pre-treatment significantly decreased levels of bacterially-induced NF-κB activation and IL-8 protein production, and differentially modulated transcript levels for IL-8, IL-1β, TNF-α and GM-CSF, TLR2, 4, 9, S100A8, S100A9, lysyl oxidase, NF-κB1, HMOX, and SOD2. Overall, the data indicate that α-lipoic acid exerts an anti-inflammatory effect on oral epithelial cells exposed to periodontal bacteria and thus may provide a novel adjunctive treatment for periodontal diseases.

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Section: Methodsmentioning

confidence: 99%

Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria

et al. 2012

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“…All experiments were performed between passages 4 and 20 using subconfluent monolayers exposed to either 1 × 10 9 heat‐killed bacteria (approximately 100 bacteria per epithelial cell; data not shown), 10 or 20 µg E.coli LPS (Sigma) per ml of culture media, or culture media alone (control) for the times stated. The ratio of bacteria to epithelia cells used in this study was based on previous analyses [29], which have indicated the maximum number of periodontal bacteria that may associate with epithelial cells from within the periodontal pocket. This approach therefore potentially enables physiological relevance for our studies with regard to periodontitis.…”

Section: Methodsmentioning

confidence: 99%

Differential activation of NF-κB and gene expression in oral epithelial cells by periodontal pathogens

Milward

Chapple

Wright

et al. 2007

Clinical and Experimental Immunology

120

141

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SummaryTo investigate the molecular effects of the periodontopathogens Fusobacterium nucleatum (FN) and Porphyromonas gingivalis (PG) on the oral epithelium, the H400 oral epithelial cell line was cultured in the presence of non-viable bacteria. Following confirmation of the presence of transcripts for the bacterial pattern recognition receptors in H400 cells, Toll-like receptors -2, -4 and -9, and components of the NF-kB signalling pathway, immunocytochemical analyses were performed showing that NF-kB was activated within 1 h of exposure to both periodontopathogens. A significantly greater number of NF-kB nuclear translocations were apparent following H400 cell exposure to FN as compared with PG. Gene expression analyses indicated that transcripts known to be regulated by the NF-kB pathway, including cytokines/chemokines TNF-a, IL-1b, IL-8, MCP-1/CCL2 and GM-CSF, were up-regulated following 4 and 24 h of exposure to both periodontopathogens. In addition, H400 periodontopathogen exposure resulted in differential regulation of transcripts for several cytokeratin gene family members. Consistent with the immunocytochemical data, microarray results indicated that FN induced a greater number of gene expression changes than PG following 24 h of exposure, 609 and 409 genes, respectively. Ninety-one genes were commonly differentially expressed by both periodontopathogens and represented biological processes commonly associated with periodontitis. Gene expression analyses by reserve transcriptase-polymerase chain reaction (RT-PCR) of molecules identified from the microarray data sets, including Heme oxygenase-1, lysyl oxidase, SOD2, CCL20 and calprotectin components, confirmed their differential expression profiles induced by the two periodontopathogens. FN and PG have clearly different molecular effects on oral epithelial cells, potentially highlighting the importance of the composition of the plaque biofilm in periodontitis pathogenesis.

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“…The World Workshop in Periodontics reported that there is sufficient evidence to incriminate Porphyromonas gingivalis , Tannerella forsythia (previously T. forsythensis ), and Aggregatibacter actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans ) as causative factors for periodontitis 1 . Studies 10–12 showed that strains of A. actinomycetemcomitans and P. gingivalis are capable of invading epithelial cells derived from human epithelial periodontal pockets or gingival sulci. A. actinomycetemcomitans also produces leukotoxin that lyses polymorphonuclear leukocytes and monocytes, which enables it to evade the innate defense line of the periodontal pocket 13 .…”

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confidence: 99%

Strong Antibacterial Effect of Miswak Against Oral Microorganisms Associated With Periodontitis and Caries

Sofrata

Claesson

Lingström

et al. 2008

Journal of Periodontology

121

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Viability of cultured periodontal pocket epithelium cells and Porphyromonas gingivalis association

Cited by 14 publications

References 64 publications

Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria

Micronutrient modulation of NF-κB in oral keratinocytes exposed to periodontal bacteria

Differential activation of NF-κB and gene expression in oral epithelial cells by periodontal pathogens

Strong Antibacterial Effect of Miswak Against Oral Microorganisms Associated With Periodontitis and Caries

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