Viability of bull spermatozoa collected from the epididymis stored at 18-20°C

Bertol, Melina Andrea Formighieri; Weiss, Romildo Romualdo; Soccol, Vanete Thomaz; Kozicki, Luiz Ernandes; Fujita, Aline Silva; Abreu, Renata Azevedo de; Green, Kerriel Thandile

doi:10.1590/s1516-89132013000500008

Cited by 16 publications

(18 citation statements)

References 24 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The results of this study revealed that viability of epididymal sperm cells declined in a storage time-dependent manner (Table-2). The values of this study were not in agreement with the findings of other researchers who recorded that only sperm motility and not live sperm cells were affected by storage temperature or post-mortem time [4,32]. On the other hand, data in this study are in accordance with the findings in bucks [38] and in red deer [39] that viability of sperm collected from the cauda epididymides post-mortem decreased progressively relative to the time of harvesting spermatozoa.…”

Section: Resultscontrasting

confidence: 99%

See 1 more Smart Citation

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

Abu¹,

Kisani²,

Ahemen³

2016

Vet World

View full text Add to dashboard Cite

Aim:Viable spermatozoa could be recovered from the cauda epididymides for the purpose of preservation of genetic material of male animals with desirable traits and for use in reproductive biotechnology. The aim of this study was to determine the effect of storage time on testicular and epididymal biometry, sperm reserves and epididymal sperm characteristics of red Sokoto bucks post mortem.Materials and Methods:Testes-epididymides were collected immediately after slaughter of mature red Sokoto bucks and transported in ice chest to the laboratory. The samples were either processed immediately or stored at 5°C in refrigerator for 24, 48 h and then processed. The testes and epididymides were measured and weighed. Sperm motility, concentration, livability, morphology, intact acrosome, and sperm reserves from different treatment groups including control were evaluated and means (±standard error of mean) were recorded.Results:There was no significant difference (p>0.05) in the testicular and epididymal dimensions determined between the means of the groups. Percent sperm motility and viability decreased significantly (p<0.05) after 24 h from 69.00±0.46 and 71.27±0.50% to 50.60±0.48 and 60.47±0.70% at 48 h, respectively. Significant decreases (p<0.05) in epididymal sperm concentration and intact acrosome from 2.86±0.08 and 92.87±0.39 at 0 to 24 h of storage, respectively, were observed.Conclusion:The results of this study suggest that spermatozoa recovered from the epididymides of red Sokoto bucks were viable after storage for up to 48 h. Furthermore, this finding offers some hope that epididymal sperm recovered post-mortem can be used in assisted reproductive technologies.

show abstract

Section: Resultscontrasting

confidence: 99%

“…Variation in quality of cauda epididymal sperm cells recovered post-mortem has been ascribed to handling conditions or species differences [31,32]. However, cold storage has the beneficial effect of keeping sperm cells viable for a longer period by slowing down the metabolic rate and degeneration of sperm cells [4,33].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of sperm recovered after slaughter from cauda epididymides of red Sokoto bucks

Abu¹,

Kisani²,

Ahemen³

2016

Vet World

View full text Add to dashboard Cite

show abstract

“…This condition has been represented in previous studies to be closer to reality, to verify the time available and establish a window of opportunity to work. A large variation in ambient temperatures was reported successfully, between 18 and 24°C [2,[7][8][9]. At a temperature of 18-20°C, it is possible to recover viable gametes, With 41.25% of progressive motility by up to 30 hours after orchiectomy [7].…”

Section: Epididymal Spermatozoamentioning

confidence: 95%

“…A large variation in ambient temperatures was reported successfully, between 18 and 24°C [2,[7][8][9]. At a temperature of 18-20°C, it is possible to recover viable gametes, With 41.25% of progressive motility by up to 30 hours after orchiectomy [7]. Thus, after the death of bulls exposed at Ambient temperature, the ideal time until sperm recovery is up to 30 hours.…”

Section: Epididymal Spermatozoamentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of Epididymal Sperm

Bertol¹

2016

Cryopreservation in Eukaryotes

View full text Add to dashboard Cite

The recovery and cryopreservation of epididymal sperm are essential tools to preserve genetic stocks of valuable domestic or wild animals under adverse conditions and also as an alternative source of gametes in cases of human infertility. This technique is recommended after death or when it is not possible to recover semen by usual methods. Sperm from the epididymis has been studied by many authors in diferent species. Cryopreservation is the most efective method for long-term preservation of genetic material from valuable breeding individuals. The protocols and diluents used for cryopreservation of conventional semen are well established, but many challenges remain working with sperm extracted directly from the epididymis. The spermatozoa retrieved from the cauda epididymis have special features, such as the absence of seminal plasma and large numbers of distal cytoplasmic droplets, which necessitate special handling, both for cryopreservation and for fertilization. For these reasons, it is important to describe in detail the features needed to cryopreserve sperm from the epididymis.

show abstract