Cryopreservation of Epididymal Sperm

Bertol, Melina Andrea Formighieri

doi:10.5772/65010

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…In the species under consideration in this review, the collection of sperm postmortem was primarily achieved through three distinct methodologies: epididymal mincing, testicular mincing, and epididymal retrograde flushing [ 47 , 52 , 53 ]. Both mincing techniques involve either the isolation of the tissue, i.e., the cauda of the epididymis or the testis, respectively, followed by cleaning and mincing in a prewarmed extender solution.…”

Section: Male Gametesmentioning

confidence: 99%

Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art

Huijsmans

Hassan

Smits

et al. 2023

Animals

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The collection of gametes from recently deceased domestic and wildlife mammals has been well documented in the literature. Through the utilization of gametes recovered postmortem, scientists have successfully produced embryos in 10 different wildlife species, while in 2 of those, offspring have also been born. Thus, the collection of gametes from recently deceased animals represents a valuable opportunity to increase genetic resource banks, obviating the requirement for invasive procedures. Despite the development of several protocols for gamete collection, the refinement of these techniques and the establishment of species–specific protocols are still required, taking into account both the limitations and the opportunities. In the case of wildlife, the optimization of such protocols is impeded by the scarcity of available animals, many of which have a high genetic value that must be protected rather than utilized for research purposes. Therefore, optimizing protocols for wildlife species by using domestic species as a model is crucial. In this review, we focused on the current advancements in the collection, preservation, and utilization of gametes, postmortem, in selected species belonging to Equidae, Bovidae, and Felidae, both domestic and wildlife.

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Section: Male Gametesmentioning

confidence: 99%

Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art

Huijsmans

Hassan

Smits

et al. 2023

Animals

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“…They can be retrieved differently depending on the species involved or epididymal size. The most used EPS retrieval methods include a) Slicing/mincing (Kaabi et al, 2003;Parra-Forero et al, 2015), b) Incision (Karja et al, 2010;Alvarez et al, 2012;Ahmed, 2019), c) Retrograde flushing via ductus deferens (resulting in less contamination) (Lorraine Leibfried-Rutledge et al, 1997;Bertol, 2016) and d) Floatation (in case of species with tiny testicles) (Bertol, 2016). Good pregnancy rates have been reported using EPS for artificial insemination in sheep (87.5%, 58.5%, and 55.0%) (Ehling et al, 2006;Rickard et al, 2014;Fernández Abella et al, 2015) and goats (61.2%) (Ocampo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

The impact of retrieval method and breed on the motility and kinematic parameters of fresh and post-thaw ram epididymal spermatozoa

Mujitaba,

Kútvölgyi,

Debnár

et al. 2024

AVet

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This study was conducted to develop ideal post-mortem gamete retrieval and conservation methods to establish a Hungarian ex-situ in vitro gene bank. Pairs of testes from German Mutton Merino (n = 7) and Hungarian Black Racka (n = 7) rams were collected at a slaughterhouse, transported to the laboratory and stored overnight (4−5 °C) before processing. Post mortem ram epididymal spermatozoa (REPS) were obtained from the cauda epididymidis by slice or incision methods. Fresh samples were extended to 200 × 106/mL cell concentration, filled into mini straws and equilibrated at 5 °C for 2 h. Freezing was performed manually in a Styrofoam box. The fresh and post-thaw total motility, progressive motility and kinematic parameters of REPS were assessed using the CASA technique. The collection method did not affect significantly the fresh and post-thaw motility and kinematic parameters. Merino had higher (P < 0.05) testicular weight. Racka had significantly better fresh and post-thaw linear movement but had statistically the same (P > 0.05) cryotolerance as Merino. In conclusion, both collection methods were found suitable for REPS retrieval. The REPS from Racka exhibited better linear movement values than those from the Merino breed. The cryotolerance of REPS of both breeds was comparable.

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“…This functional epididymal sperm (ES) maturation occurs in response to modifications in the epididymal fluid (EF) microenvironment [6]. The ES surface during transit along the epididymis is directly exposed to an epididymal milieu rich in numerous proteins, which may interact with the sperm in a specific manner [4,7]. This may have an impact on the functional features of the male reproductive cells [8].…”

Section: Introductionmentioning

confidence: 99%

“…Taking into consideration that the epididymis provides a unique environment in which sperm acquire the fertilizing ability [7], it was decided to: (i) Provide profiling of the canine EF proteome; (ii) Investigate correlations between cauda EF proteins and selected ES motion parameters in the search for protein markers of ES motility.…”

Section: Introductionmentioning

confidence: 99%

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

Cichowska,

Wisniewski,

Bromke

et al. 2023

IJMS

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Sperm maturation in the epididymis is based on interactions with proteins from epididymal fluid (EF). The aim of the study was to profile canine EF proteome and investigate correlations between EF protein content and epididymal spermatozoa (ES) motion parameters. Twenty-three male dogs were divided into two groups: good sperm motility (GSM) and poor sperm motility (PSM). The total motility and progressive motility differed significantly (p = 0.031; p < 0.001, respectively) between the GSM group and the PSM group. The semen samples were centrifuged to separate the EF apart from the ES. The canine EF proteins were analyzed using nano-liquid chromatography, which was coupled with quadrupole time-of-flight mass spectrometry (NanoUPLC-Q-TOF/MS) and bioinformatic tools for the first time. A total of 915 proteins were identified (GSM—506; PSM—409, respectively). UniProt identification resulted in six unique proteins (UPs) in the GSM group of dogs and four UPs in the PSM group. A semi-quantitative analysis showed a higher abundance (p < 0.05) of four differentially expressed proteins in the GSM group (ALB, CRISP2, LCNL1, PTGDS). Motility-dependent variations were detected in the EF proteome and were related to important metabolic pathways, which might suggest that several proteins could be potential ES motility biomarkers.

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Cryopreservation of Epididymal Sperm

Cited by 6 publications

References 30 publications

Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art

Postmortem Collection of Gametes for the Conservation of Endangered Mammals: A Review of the Current State-of-the-Art

The impact of retrieval method and breed on the motility and kinematic parameters of fresh and post-thaw ram epididymal spermatozoa

Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility

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