Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining

Collins, Anita M.; Donoghue, Ann M.

doi:10.1016/s0093-691x(99)00094-1

Cited by 114 publications

(103 citation statements)

References 29 publications

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“…Propidium iodide is normally used as the viability probe of choice in FC as this supravital stain rapidly penetrates non-viable spermatozoa when their plasma membrane is disrupted. 30 This FC procedures (available as a live/dead kit from Invitrogen (Carlsbad, CA, USA)) have been successfully applied for many species, as human, 31 bovine, 32 porcine, 13,31,32 ovine, 31 Lagomorpha, 31 murine, 31,33 avian, 34,35 honey bees 36,37 and fish. 38 Furthermore, it is able to simultaneously evaluate sperm cell viability together with some other attributes, e.g., in combination with fluorescently labeled plant lectins for simultaneous assessment of plasma membrane and acrosome integrity.…”

Section: Sperm Intactnessmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

140

120

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Flow cytometry is now a recognized methodology within animal spermatology, and has moved from being a research tool to become routine in the assessment of animal semen destined to breeding. The availability of 'bench-top' flow cytometers and of newer and versatile markers for cell structure and function had allowed the instrumentation to measure more sperm parameters, from viability to reactiveness when exposed to exogenous stimuli, and to increase our capabilities to sort spermatozoa for potential fertilizing capacity, or chromosomal sex. The present review summarizes the state of the art regarding flow cytometry applied to animal andrology, albeit keeping an open comparative intent. It critically evaluates the present and future capabilities of flow cytometry for the diagnostics of potential fertility and for the development of current reproductive technologies such as sperm freezing, sperm selection and sperm sorting. The flow cytometry methods will probably further revolutionize our understanding of the sperm physiology and their functionality, and will undoubtedly extend its application in isolating many uncharacterized features of spermatozoa. However, continuous follow-up of the methods is a necessity owing to technical developments and the complexity of mapping spermatozoa.

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Section: Sperm Intactnessmentioning

confidence: 99%

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Hossain¹,

Johannisson²,

Wallgren³

et al. 2011

Asian J Androl

140

120

View full text Add to dashboard Cite

show abstract

“…The kit contains a membrane-permeant nucleic acid stain (SYBR®14 dye with a maximum fluorescence emission of 516 nm) and a conventional dead-cell stain (propidium iodide with a maximum fluorescence emission of 617 nm). The kit was developed for mammal sperm, but has been successfully modified for Arthopoda (honey bee, Apis mellifera linnaeus, 1758) using less concentrated dyes (Collins & donoghue 1999). As proposed by Collins & donoghue (1999), SYBR®14 dye diluted at 1 µl/litre and propidium iodide diluted at 5 µl/litre were used.…”

Section: Sperm Viabilitymentioning

confidence: 99%

“…The kit was developed for mammal sperm, but has been successfully modified for Arthopoda (honey bee, Apis mellifera linnaeus, 1758) using less concentrated dyes (Collins & donoghue 1999). As proposed by Collins & donoghue (1999), SYBR®14 dye diluted at 1 µl/litre and propidium iodide diluted at 5 µl/litre were used. For lobster, sperm were incubated at 4°C to avoid killing live sperm (i.e., condition similar to nature).…”

Section: Sperm Viabilitymentioning

confidence: 99%

Internal organ pathology of wild American lobster (Homarus americanus) from eastern Canada affected with shell disease

Comeau

Benhalima

2009

New Zealand Journal of Marine and Freshwater Research

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For the first time in decades, multiple lesions on the exoskeleton of wild caught American lobsters (Homarus americanus) were observed in eastern Canada in 2005 and 2006. These lesions were similar to those observed since the mid 1990s in the eastern United States. Of the 12 lobsters examined, 8 were males. Scanning electron microscopy showed a dense population of rod-shaped bacteria associated with the breakdown of the first layer of cuticle. However, the ability of bacteria to fully penetrate the carapace is unknown. The shell disease did not seem to be immediately fatal and was often associated with alterations in the histoarchitecture of several internal organs (i.e., testis, vas deferens, hepatopancreas, and gills). For instance, follicle cells responsible for spermatogenesis were damaged, resulting in deformed and non-viable spermatozoa in the testis and vas deferens. In contrast, the female reproductive organ did not show any abnormalities. R-cells responsible for the storage of lipids and glycogen in the hepatopancreas were severely damaged or destroyed, indicating stress and starvation. Beside necrosis and calcification of gill filaments, debris between the gill filaments was also observed that would limit ion exchange and cause metabolic exhaustion. It does not seem that shell disease was the causative agent or a vector for the pathological conditions of internal organs, but one of the symptoms of a common agent affecting the internal organs. Although shell disease was observed in several areas in eastern Canada, its prevalence M07111;

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“…The queen bee retains about 4.5 to 5.7 million sperm in the spermatheca (4,5). This quantity is sufficient to fertilize egg cells for her entire life, as sperm can be stored in the spermatheca for several years (6,7). In the 1930s, the first successful artificial insemination of queen bees was carried out in the United States.…”

Section: Introductionmentioning

confidence: 99%

“…In the 1930s, the first successful artificial insemination of queen bees was carried out in the United States. Currently, artificial insemination of bees is used primarily for research purposes and in breeding work for the maintenance and selection of stocks with specific traits (6,8). During insemination the queen receives (depending on the method) from 2 to 10 mm 3 of semen in 1-3 doses.…”

Section: Introductionmentioning

confidence: 99%

Differences in drone sperm morphometry and activity at the beginning and end of the season

Gontarz¹,

Banaszewska²,

Gryzińska³

et al. 2016

Turk J Vet Anim Sci

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Viability assessment of honey bee, Apis mellifera, sperm using dual fluorescent staining

Cited by 114 publications

References 29 publications

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Flow cytometry for the assessment of animal sperm integrity and functionality: state of the art

Internal organ pathology of wild American lobster (Homarus americanus) from eastern Canada affected with shell disease

Differences in drone sperm morphometry and activity at the beginning and end of the season

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