Differences in drone sperm morphometry and activity at the beginning and end of the season

Gontarz, A.; Banaszewska, Dorota; Gryzińska, Magdalena; Andraszek, Katarzyna

doi:10.3906/vet-1511-6

Cited by 22 publications

(23 citation statements)

References 22 publications

(20 reference statements)

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“…These differences were, however, nonsignificant. This would confirm the hypothesis, as the sperm cells from the spring had larger dimensions (28). According to Gomendio and Roldan (29) and Mossman et al (30), longer mammalian spermatozoa are more competitive in terms of fertilization.…”

Section: Discussionsupporting

confidence: 76%

Effects of season on semen parameters and relationships between selected semen characteristics in Hypor boars

Górski¹,

Kondracki²,

Wysokińska³

2017

Turk J Vet Anim Sci

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IntroductionPorcine ejaculate performance and semen quality depend on factors such as age, breed, feeding and nutrition, housing conditions, animal care, the way the semen is collected, and many others (1,2). Libido also affects ejaculate traits (3). Reproductive performance of artificial insemination (AI) in boars stems largely from semen quality, which is a function of the incidence of sperm cell morphological abnormalities (4). Besides the semen quality, the AI value of semen is additionally influenced by sperm morphometric features, their sizes, and shapes (5). One of the methods used to assess the biological value of semen and its suitability for inseminating females is examination of sperm morphology (6). In AI practice cases of reduced male fertility are observed. This may be due to seasonal factors such as temperature, humidity, and light intensity (1). The occurrence of seasonal variation in the frequency of morphological sperm anomalies may depend on the breed of pig (2). This is because of varying degrees of sensitivity among breeds to the effects of seasonal factors (7).The aim of this study was to determine the effects of seasonal factors on ejaculate physical traits and sperm morphology in Hypor boars and to establish correlations between some semen traits and year seasons. Materials and methodsThe analysis involved 114 ejaculates collected from 12 Hypor boars utilized in three AI centers located in central Poland. Geographically the area ranges between latitudes 51°60′N and 52°50′N and between 19°10′E and 20°30′E. The average altitude of the area is 109 m above sea level. Four calendar-based seasons of the year were considered:

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Section: Discussionsupporting

confidence: 76%

Effects of season on semen parameters and relationships between selected semen characteristics in Hypor boars

Górski¹,

Kondracki²,

Wysokińska³

2017

Turk J Vet Anim Sci

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“…The spermatozoa of A. mellifera, as described by Peng [9] are long and filamentous, with a total length of about 250-270 μm, the head measures 8-10 μm and the total length of the acrosomal complex measures 5 μm. A study by Gontarz et al [10] has focused the attention on the analysis of Apis mellifera carnica spermatozoa and the results are shown in Table 2.…”

Section: Resultsmentioning

confidence: 99%

Morphological and morphometric analysis of the Italian honeybee (Apis mellifera ligustica) spermatozoa: a preliminary study in Campania region

Power¹,

D’Anza²,

Martano³

et al. 2019

Vet Med Anim Sci

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Background: Since few decades, the world is facing important losses in the number of honeybees, with great threat to the agro-zootechnic economics and to the global biodiversity. It is well known that stressors can affect the female and male reproductive system, impairing queens' and drones' fertility. To date, still very little is known about drones' physiological characteristics and possible alterations of the reproductive activity. This study focused on describing the morphological features and morphometric parameters of the Italian honeybee (Apis mellifera ligustica) spermatozoa in order to define its standard characteristics. Findings: The following morphometric values (mean ± standard deviation) were measured: sperm total length (230,81±17,22 μm), tail length (222,96±17,15μm), head length (7,85±0,65μm), nucleus length (4,44±0,61μm) and perforator length (3,58±1,21μm). Moreover, 7% of the spermatozoa exhibited alterations such as broken and double tails. Conclusions: The results obtained, allow a preliminary definition of measures of A. mellifera ligustica spermatozoa using an easy and economical protocol that could help to study the morphology and the morphometry of drone spermatozoa and to point out possible alterations.

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“…As silver nitrate is an alkaline dye, it is mainly used to identify acidic chromatin proteins and the chromatin of nucleolus organizer regions in mitotic chromosomes (Andraszek, Horoszewicz, & Smalec, ), as well as nucleoli during meiosis (Andraszek, Gryzińska, Wójcik, Knaga, & Smalec, ; Andraszek, Szeleszczuk, Niedbała, & Kuchta‐Gładysz, ). The basic methodology has been modified in our Department (Department of Animal Genetics and Horse Breeding, University of Natural Sciences and Humanities in Siedlce) and successfully used to identify morphological details of spermatozoa from fresh, frozen or fixed semen samples, from mammals (Andraszek, Banaszewska, et al, ; Andraszek & Smalec, ; Banaszewska, Andraszek, Czubaszek, et al, ; Banaszewska, Andraszek, Szostek, et al, ; Banaszewska, Andraszek, Zdrowowicz, et al, ) birds (Andraszek et al, ) and insects (Gontarz, Banaszewska, Gryzińska, & Andraszek, ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Andraszek

Banaszewska

Szeleszczuk

et al. 2020

Reprod Domestic Animals

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Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitrate staining, the sperm isolated from the tail of the epididymis were characterized by dark ‘collars’ in the distal part of the head. These ‘collars’ are not visible in the sperm cells isolated from semen. The results of the study indicate differences in the dimensions of sperm isolated from the tail of the epididymis and sperm in semen. Sperm isolated from the tail of the epididymis had smaller heads, despite their longer length, and had longer midpieces and tails than ejaculate sperm. Silver nitrate staining is a simple and fast technique. Silver nitrate makes it possible to identify the acrosome and post‐acrosomal region of the sperm head and to clearly identify the midpiece. Therefore, it can be successfully used to supplement routine techniques for evaluating sperm morphology or as an independent technique.

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Differences in drone sperm morphometry and activity at the beginning and end of the season

Cited by 22 publications

References 22 publications

Effects of season on semen parameters and relationships between selected semen characteristics in Hypor boars

Effects of season on semen parameters and relationships between selected semen characteristics in Hypor boars

Morphological and morphometric analysis of the Italian honeybee (Apis mellifera ligustica) spermatozoa: a preliminary study in Campania region

Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

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