Viability and neuronal differentiation of neural stem cells encapsulated in silk fibroin hydrogel functionalized with an IKVAV peptide

Sun, Wei; Incitti, Tania; Migliaresi, Claudio; Quattrone, Alessandro; Casarosa, Simona; Motta, Antonella

doi:10.1002/term.2053

Cited by 106 publications

(87 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Silk is produced by silkworm (Bombyx mori) that comprises 75% fibroin and 25% sericin and carbohydrates . Silk is biocompatible, biodegradable and has good mechanical properties that because of these features is used in various industries, and also commonly used in the medicine as surgical sutures. Because of high biocompatibility of silk, it can be used as a scaffold for increasing the growth of many stem cells .…”

Section: Discussionmentioning

confidence: 99%

“…Silk is biocompatible, biodegradable and has good mechanical properties that because of these features is used in various industries, and also commonly used in the medicine as surgical sutures. Because of high biocompatibility of silk, it can be used as a scaffold for increasing the growth of many stem cells . Moreover, degradation of silk is 6 months in the body and within 2 years is completely decomposed, so it can be used as a good scaffold in tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Bojnordi

Ebrahimi‐Barough

Vojoudi

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

The scaffolds accompanied with stem cells have great potential for applications in neural tissue engineering. Fabrication of nanofibrous scaffold similar to extracellular matrix is one of the applicable methods in neural tissue regeneration. The aim of this study was the fabrication of a silk nanofibrous scaffold as a microenvironment for neural guiding differentiation of embryonic stem like cells (ES Like cells) derived from testis toward neuron-like cells. ES Like derived from culturing of testicular cells in vitro, were seeded on silk scaffolds and induced to neuronal phenotype using 4-/4± RA technique following culturing the cells in the neurobasal medium supplemented with 20 ng/mL bFGF,10 ng/mL EGF, B27, and N2 for 8-12 days. The neural differentiation was confirmed via the evaluation of specific neural markers; Nestin, NF68, MAP2 and β tubulin using immunocytochemistry and real-time polymerase chain reaction. Our results showed that silk scaffold support the attachment and proliferation of ES Like cells. The expression of Nestin, NF68, Map2, and ß tubulin markers were higher in cells grown on silk scaffold in compare to monolayer group. This study suggests electrospun silk nanofibrous scaffold as an appropriate substrate for neural induction of stem cells that is applicable for repairmen of damaged neural tissues. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2662-2669, 2018.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Bojnordi

Ebrahimi‐Barough

Vojoudi

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…The storage modulus of the IKVAV‐modified silk fibroin hydrogel was increased from 1.3 to 2.9 kPa. Neuronal stem cells encapsulated in the modified hydrogel showed higher β3 tubulin and MAP 2 expression …”

Section: Cell Culture and Differentiationmentioning

confidence: 99%

Ile‐Lys‐Val‐ala‐Val (IKVAV) peptide for neuronal tissue engineering

Patel

Santhosh

Dash

et al. 2018

Polymers for Advanced Techs

View full text Add to dashboard Cite

Despite the great advances in microsurgery, some neural injuries cannot be treated surgically. Stem cell therapy is a potential approach for treating neuroinjuries and neurodegenerative disease. Researchers have developed various bioactive scaffolds for tissue engineering, exhibiting enhanced cell viability, attachment, migration, neurite elongation, and neuronal differentiation, with the aim of developing functional tissue grafts that can be incorporated in vivo. Facilitating the appropriate interactions between the cells and extracellular matrix is crucial in scaffold design. Modification of scaffolds with biofunctional motifs such as growth factors, drugs, or peptides can improve this interaction. In this review, we focus on the laminin‐derived Ile‐Lys‐Val‐Ala‐Val peptide as a biofunctional epitope for neuronal tissue engineering. Inclusion of this bioactive peptide within a scaffold is known to enhance cell adhesion as well as neuronal differentiation in both 2‐dimensional and 3‐dimensional environments. The in vivo application of this peptide is also briefly described.

show abstract

“…The effect of IKVAV in neuronal differentiation and tissue engineering was assessed by comparing natural silk fibroin hydrogel covalently modified with IKVAV with unmodified hydrogel. The study showed that the hydrogel modified with IKVAV can increase cell viability and neuronal differentiation when compared with cells encapsulated with silk fibroin hydrogel alone (Sun et al, ). Recently, studies with synthetic PuraMatrix™ covalently combined with the IKVAV motif showed promising results by differentiating NSCs toward neurons and astrocytes without the addition of growth factors (Sun et al, ).…”

Section: Biomaterialsmentioning

confidence: 99%

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Groot

Sliedregt

Benthem

et al. 2019

The Anatomical Record

View full text Add to dashboard Cite

In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

show abstract

Viability and neuronal differentiation of neural stem cells encapsulated in silk fibroin hydrogel functionalized with an IKVAV peptide

Cited by 106 publications

References 47 publications

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Silk nanofibrous electrospun scaffold enhances differentiation of embryonic stem like cells derived from testis in to mature neuron

Ile‐Lys‐Val‐ala‐Val (IKVAV) peptide for neuronal tissue engineering

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Contact Info

Product

Resources

About