Ile‐Lys‐Val‐ala‐Val (IKVAV) peptide for neuronal tissue engineering

Patel, Rajkumar; Santhosh, Mallesh; Dash, Jatis Kumar; Karpoormath, Rajshekhar; Jha, Amitabh; Kwak, Jeonghun; Patel, Madhumita; Kim, Jong Hak

doi:10.1002/pat.4442

Cited by 35 publications

(37 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, the MDA-MB231 cell line is derived from metastatic ductal breast carcinoma and presents abundant stroma with fibrous consistency [44]. Both HCC1954 and MDA-MB231 breast cancer cell lines were grown in a collagen-free scaffold based on crosslinked and lyophilized matrix components, such as hyaluronic acid and ioniccomplementary self-assembling peptides (SAPs) condensed with the IKVAV (Ile-Lys-Val-Ala-Val) Laminin adhesion motif [45][46][47]. We previously demonstrated that it could reliably reproduce in vivo tissue architecture [48,49].…”

Section: Morphology Of Hcc1954 and Mda-mb231 3d Cell Culturesmentioning

confidence: 99%

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

Sieni

Dettin

Robertis

et al. 2020

Cancers

View full text Add to dashboard Cite

Gene Electro-Transfer (GET) is a powerful method of DNA delivery with great potential for medical applications. Although GET has been extensively studied in vitro and in vivo, the optimal parameters remain controversial. 2D cell cultures have been widely used to investigate GET protocols, but have intrinsic limitations, whereas 3D cultures may represent a more reliable model thanks to the capacity of reproducing the tumor architecture. Here we applied two GET protocols, using a plate or linear electrode, on 3D-cultured HCC1954 and MDA-MB231 breast cancer cell lines grown on a novel collagen-free 3D scaffold and compared results with conventional 2D cultures. To evaluate the electrotransfer efficiency, we used the plasmid pEGFP-C3 encoding the enhanced green fluorescent protein (EGFP) reporter gene. The novel 3D scaffold promoted extracellular matrix deposition, which particularly influences cell behavior in both in vitro cell cultures and in vivo tumor tissue. While the transfection efficiency was similar in the 2D-cultures, we observed significant differences in the 3D-model. The transfection efficiency in the 3D vs 2D model was 44% versus 15% (p < 0.01) and 24% versus 17% (p < 0.01) in HCC1954 and MDA-MB231 cell cultures, respectively. These findings suggest that the novel 3D scaffold allows reproducing, at least partially, the peculiar morphology of the original tumor tissues, thus allowing us to detect meaningful differences between the two cell lines. Following GET with plate electrodes, cell viability was higher in 3D-cultured HCC1954 (66%) and MDA-MB231 (96%) cell lines compared to their 2D counterpart (53% and 63%, respectively, p < 0.001). Based on these results, we propose the novel 3D scaffold as a reliable support for the preparation of cell cultures in GET studies. It may increase the reliability of in vitro assays and allow the optimization of GET parameters of in vivo protocols.

show abstract

Section: Morphology Of Hcc1954 and Mda-mb231 3d Cell Culturesmentioning

confidence: 99%

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

Sieni

Dettin

Robertis

et al. 2020

Cancers

View full text Add to dashboard Cite

show abstract

“…In this study, we present a new collagen-free 3D scaffold for cell culture aimed at reproducing as close as possible the tissue organization commonly found in tumors in vivo [30,31]. The new scaffold is a crosslinked and lyophilized matrix based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs) condensed with the IKVAV (Ile-Lys-Val-Ala-Val) Laminin adhesion motif [32,33,34]. The proposed collagen-free scaffold promotes HCC1569 and MDA-MB231 growth and induces cellular production of ECM.…”

Section: Introductionmentioning

confidence: 99%

A Novel 3D Scaffold for Cell Growth to Asses Electroporation Efficacy

Dettin

Sieni

Zamuner

et al. 2019

Cells

View full text Add to dashboard Cite

Tumor electroporation (EP) refers to the permeabilization of the cell membrane by means of short electric pulses thus allowing the potentiation of chemotherapeutic drugs. Standard plate adhesion 2D cell cultures can simulate the in vivo environment only partially due to lack of cell–cell interaction and extracellular matrix (ECM). In this study, we assessed a novel 3D scaffold for cell cultures based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs), by studying the growth patterns of two different breast carcinoma cell lines (HCC1569 and MDA-MB231). This 3D scaffold modulates cell shape and induces extracellular matrix deposit around cells. In the MDA-MB 231 cell line, it allows three-dimensional growth of structures known as spheroids, while in HCC1569 it achieves a cell organization similar to that observed in vivo. Interestingly, we were able to visualize the electroporation effect on the cells seeded in the new scaffold by means of standard propidium iodide assay and fluorescence microscopy. Thanks to the presence of cell–cell and cell–ECM interactions, the new 3D scaffold may represent a more reliable support for EP studies than 2D cancer cell cultures and may be used to test new EP-delivered drugs and novel EP protocols.

show abstract

“…This osteoconduction property of IKVAV peptide was already reported by Frith et al (2012) [35]. The combination of properties encompassing different tissues makes IKVAV an interesting candidate to increase biological function to biomaterials [recently reviewed in [36]].…”

Section: In Vivo Evaluationmentioning

confidence: 54%

“…In addition, there is an increase in vessel density over time in IKVAV including YIGSR, RNIAEIIKDI and especially IKVAV, which were described to promote cell attachment and/or neurite outgrowth and axon guidance [29,30]. It was recently demonstrated that inclusion of IKVAV within a scaffold is known to enhance cell adhesion of neurons, as well as neuronal differentiation in both 2-dimensional and 3-dimensional environments [reviewed in [31] ] .…”

Section: In Vivo Evaluationmentioning

confidence: 99%

Development of a cell-free and growth factor-free hydrogel capable of inducing angiogenesis and innervation after subcutaneous implantation

et al. 2019

View full text Add to dashboard Cite

delayed or impaired recovery of bone defects. The design of innovative biomaterials should improve the host capacity of healing to restore a functional tissue, taking into account that the nerve systems closely interact with blood vessels in the bone tissue. The aim of this work is to develop a cell-free and growth factor-free hydrogel capable to promote angiogenesis and innervation. To this end, we have used elastin-like polypeptides (ELPs), poly(ethylene glycol) (PEG) and increasing concentrations of the adhesion peptide IKVAV (25 % (w/w) representing 1.7 mM and 50 % (w/w) representing 4.1 mM) to formulate and produce hydrogels. When characterized in vitro, hydrogels have fine-tunable rheological properties, microporous structure and are biocompatible. At the biological level, 50 % IKVAV composition up-regulated Runx2, Osx, Spp1, Vegfa and Bmp2 in mesenchymal stromal cells and Tek in endothelial cells, and sustained the formation of long neurites in sensory neurons. When implanted subcutaneously in mice, hydrogels induced no signals of major inflammation and the 50 % IKVAV composition induced higher vessel density and formation of nervous terminations in the peripheral tissue. This novel composite has important features for tissue engineering, showing higher osteogenic, angiogenic and innervation potential in vitro, being not inflammatory in vivo, and inducing angiogenesis and innervation subcutaneously.

show abstract

Ile‐Lys‐Val‐ala‐Val (IKVAV) peptide for neuronal tissue engineering

Cited by 35 publications

References 53 publications

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold

A Novel 3D Scaffold for Cell Growth to Asses Electroporation Efficacy

Development of a cell-free and growth factor-free hydrogel capable of inducing angiogenesis and innervation after subcutaneous implantation

Contact Info

Product

Resources

About