Vesicle-Mediated Solute Transport between the Vacuole and the Plasma Membrane

Echeverrı́a, Ed

doi:10.1104/pp.123.4.1217

Cited by 60 publications

(34 citation statements)

References 52 publications

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“…It remains to be established whether these results indicate that, similar to the finding for the synthesis of alkaloids in Papaver (Roberts et al 1983), phenylpropanoid biosynthetic enzymes might be sequestered in specialized structures, minimizing intermediate loss and contributing to trafficking. Substantial evidence also supports the vesicle-mediated transport of various compounds between the vacuole and the plasma membrane (Echeverria 2000).…”

Section: Of Some Of the 129 Abc Transporters (Sanchez-fernandez Et Almentioning

confidence: 81%

The challenges of moving chemicals within and out of cells: insights into the transport of plant natural products

Grotewold

2004

Planta

103

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Section: Of Some Of the 129 Abc Transporters (Sanchez-fernandez Et Almentioning

confidence: 81%

The challenges of moving chemicals within and out of cells: insights into the transport of plant natural products

Grotewold

2004

Planta

103

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“…Their involvement is implicated by apoplastic steps of post-phloem transfer in maize kernels, but symplastic roles of these transporters can also include vesicle-based transfer processes (Echeverría, 2000; Lalonde et al, 2004), transient storage by vacuoles (Eom et al, 2011), cellular efflux (Lalonde et al, 2004; Chen et al, 2010, 2012; Baker et al, 2012; Braun, 2012), or action as sensors (Kang and Turano, 2003; Lalonde et al, 2004; Slewinski, 2011; Aoki et al, 2012; Price et al, 2012). In maize kernels, prominent contributions are indicated for both sucrose and hexose transporters (Aoki et al, 2012), and the importance of SWEET-mediated efflux to diverse sinks (Chen et al, 2010, 2012), led to suggested roles in efflux to seeds (Braun, 2012).…”

Section: Molecular Contributors To Strong Sink Strength In Grainsmentioning

confidence: 99%

Regulation of assimilate import into sink organs: update on molecular drivers of sink strength

et al. 2013

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Recent developments have altered our view of molecular mechanisms that determine sink strength, defined here as the capacity of non-photosynthetic structures to compete for import of photoassimilates. We review new findings from diverse systems, including stems, seeds, flowers, and fruits. An important advance has been the identification of new transporters and facilitators with major roles in the accumulation and equilibration of sugars at a cellular level. Exactly where each exerts its effect varies among systems. Sugarcane and sweet sorghum stems, for example, both accumulate high levels of sucrose, but may do so via different paths. The distinction is central to strategies for targeted manipulation of sink strength using transporter genes, and shows the importance of system-specific analyses. Another major advance has been the identification of deep hypoxia as a feature of normal grain development. This means that molecular drivers of sink strength in endosperm operate in very low oxygen levels, and under metabolic conditions quite different than previously assumed. Successful enhancement of sink strength has nonetheless been achieved in grains by up-regulating genes for starch biosynthesis. Additionally, our understanding of sink strength is enhanced by awareness of the dual roles played by invertases (INVs), not only in sucrose metabolism, but also in production of the hexose sugar signals that regulate cell cycle and cell division programs. These contributions of INV to cell expansion and division prove to be vital for establishment of young sinks ranging from flowers to fruit. Since INV genes are themselves sugar-responsive “feast genes,” they can mediate a feed-forward enhancement of sink strength when assimilates are abundant. Greater overall productivity and yield have thus been attained in key instances, indicating that even broader enhancements may be achievable as we discover the detailed molecular mechanisms that drive sink strength in diverse systems.

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“…As a result, the protonated form cannot move across a tonoplast membrane. However, two distinct pathways mediated by carrier or vesicular transport have been used to explain the transport mechanism of solute from vacuole (for review, see Echeverria, 2000). …”

Section: Subcellular Localization Of Cpt In Hairy Roots Of O Pumilamentioning

confidence: 99%

Transport of camptothecin in hairy roots of Ophiorrhiza pumila

et al. 2007

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We have investigated the subcellular accumulation and transport of camptothecin (CPT), a monoterpene indole alkaloid, in hairy roots of Ophiorrhiza pumila. This hairy root produces high amounts of CPT and excretes it into the culture medium. When the hairy roots were exposed to UV radiation, autofluorescence emitted from CPT showed subcellular localization of CPT in the vacuole. Treatment with several inhibitors suggested that CPT excretion is a transporter-independent passive transport controlled by the concentration gradient of the compound. Interestingly, the hairy roots treated with brefeldin A, a vesicle transport inhibitor, showed increased CPT excretion. This could be explained by an increased transport rate of CPT from the endoplasmic reticulum (ER) to the cytoplasm when transport of CPT to the vacuole is blocked. The much higher concentration of CPT in the cytoplasm resulted in the increased excretion rate. This result indicates that CPT is biosynthesized at the ER and transported to accumulate in the vacuole by the same machinery that is used for vacuolar protein sorting. How O. pumila is insensitive to CPT is discussed.

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Vesicle-Mediated Solute Transport between the Vacuole and the Plasma Membrane

Cited by 60 publications

References 52 publications

The challenges of moving chemicals within and out of cells: insights into the transport of plant natural products

The challenges of moving chemicals within and out of cells: insights into the transport of plant natural products

Regulation of assimilate import into sink organs: update on molecular drivers of sink strength

Transport of camptothecin in hairy roots of Ophiorrhiza pumila

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