Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation

Janssen, Lauriane; Saranya, Muthusamy; Leinonen, Marko E.; Pitkänen, Olli; Mobasheri, Ali; Lorite, Gabriela S.

doi:10.1016/j.carbon.2019.11.040

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…The proliferation of chondrocytes to achieve unidirectional orientation was controllable by optimizing the size and spacing of VA–MWCNT micropillars. Cell attachment, proliferation, and ECM production reportedly were enhanced by micropillars in comparison to the control groups [ 137 ].…”

Section: Reviewmentioning

confidence: 99%

“…Due to the specific orientation of chondrocytes, tissue engineering faces a limitation to mimic the architecture of cartilage. Therefore, Janssen et al used VA–MWCNT micropillars to induce an unidirectional orientation of chondrocytes [ 137 ]. The Young’s modulus of the VA–MWCNT micropillars was in the range of the natural ECM of articular cartilage.…”

Section: Reviewmentioning

confidence: 99%

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Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

Nabizadeh¹,

Nasrollahzadeh²,

Daemi³

et al. 2022

Beilstein J. Nanotechnol.

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Osteoarthritis, which typically arises from aging, traumatic injury, or obesity, is the most common form of arthritis, which usually leads to malfunction of the joints and requires medical interventions due to the poor self-healing capacity of articular cartilage. However, currently used medical treatment modalities have reported, at least in part, disappointing and frustrating results for patients with osteoarthritis. Recent progress in the design and fabrication of tissue-engineered microscale/nanoscale platforms, which arises from the convergence of stem cell research and nanotechnology methods, has shown promising results in the administration of new and efficient options for treating osteochondral lesions. This paper presents an overview of the recent advances in osteochondral tissue engineering resulting from the application of micro- and nanotechnology approaches in the structure of biomaterials, including biological and microscale/nanoscale topographical cues, microspheres, nanoparticles, nanofibers, and nanotubes.

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Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

Nabizadeh¹,

Nasrollahzadeh²,

Daemi³

et al. 2022

Beilstein J. Nanotechnol.

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“…Arranged in a ring formation, PDMS micropillars deflected in response to the forces exerted by growing spheroids located in the center. Micropillar array technologies have also been adapted for use with unconventional materials such as carbon nanotubes in replacement of typical elastomeric materials like PDMS . By mimicking the mechanical properties of native cartilage, Janssen and colleagues were able to use this platform to measure the contractile forces exerted by chondrocyte cells on the micropillars, as well as facilitate the unidirectional orientation of chondrocytes (Figure , panel c), which is normally a key obstacle in cartilage tissue engineering processes.…”

Section: Measuring Tractions In 2d Environmentsmentioning

confidence: 99%

Section: Measuring Tractions In 2d Environmentsmentioning

confidence: 99%

Physicochemical Tools for Visualizing and Quantifying Cell-Generated Forces

Nguyen

Kilian

2020

ACS Chem. Biol.

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To discern how mechanical forces coordinate biological outcomes, methods that map cell-generated forces in a spatiotemporal manner, and at cellular length scales, are critical. In their native environment, whether it be within compact multicellular three-dimensional structures or sparsely populated fibrillar networks of the extracellular matrix, cells are constantly exposed to a slew of physical forces acting on them from all directions. At the same time, cells exert highly localized forces of their own on their surroundings and on neighboring cells. Together, the generation and transmission of these forces can control diverse cellular activities and behavior as well as influence cell fate decisions. To thoroughly understand these processes, we must first be able to characterize and measure such forces. However, our experimental needs and technical capabilities are in discordwhile it is apparent that we should study cell-generated forces within more biologically relevant 3D environments, this goal remains challenging because of caveats associated with complex "sensing−transduction−readout" modalities. In this Review, we will discuss the latest techniques for measuring cell-generated forces. We will highlight recent advances in traction force microscopy and examine new alternative approaches for quantifying cell-generated forces, both of individual cells and within 3D tissues. Finally, we will explore the future direction of novel cellular force-sensing tools in the context of mechanobiology and next-generation biomaterials design.

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“…[14][15][16][17][18] Carbon nanotubes (CNTs) have emerged as a promising contender as scaffolds for the production of engineered cartilage. [19][20][21][22][23][24][25][26][27][28][29] This is largely due to their intrinsic ability to provide fibrous nanoscale topography resembling that of the ECM, 19,20,22 simulating the immediate environment that chondrocytes interact with in vivo. The addition of nanoscale topography has been shown to increase cell adhesion 19,24 and improve chondrocyte proliferation.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic approach to articular cartilage tissue engineering using carbon nanotube–coated and textured polydimethylsiloxane scaffolds

Elídóttir

Scott

Lewis

et al. 2022

Annals of the New York Academy of Sciences

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There is a significant need to understand the complexity and heterogeneity of articular cartilage to develop more effective therapeutic strategies for diseases such as osteoarthritis. Here, we show that carbon nanotubes (CNTs) are excellent candidates as a material for synthetic scaffolds to support the growth of chondrocytes—the cells that produce and maintain cartilage. Chondrocyte morphology, proliferation, and alignment were investigated as nanoscale CNT networks were applied to macroscopically textured polydimethylsiloxane (PDMS) scaffolds. The application of CNTs to the surface of PDMS‐based scaffolds resulted in an up to 10‐fold increase in cell adherence and 240% increase in proliferation, which is attributable to increased nanoscale roughness and hydrophilicity. The introduction of macroscale features to PDMS induced alignment of chondrocytes, successfully mimicking the cell behavior observed in the superficial layer of cartilage. Raman spectroscopy was used as a noninvasive, label‐free method to monitor extracellular matrix production and chondrocyte phenotype. Chondrocytes on these scaffolds successfully produced collagen, glycosaminoglycan, and aggrecan. This study demonstrates that introducing physical features at different length scales allows for a high level of control over tissue scaffold design and, thus, cell behavior. Ultimately, these textured scaffolds can serve as platforms to improve the understanding of osteoarthritis and for early‐stage therapeutic testing.

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Vertically aligned carbon nanotube micropillars induce unidirectional chondrocyte orientation

Cited by 6 publications

References 35 publications

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

Physicochemical Tools for Visualizing and Quantifying Cell-Generated Forces

Biomimetic approach to articular cartilage tissue engineering using carbon nanotube–coated and textured polydimethylsiloxane scaffolds

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