Carbohydrates have been shown to attain 25% of the organic matter pool during periods of high productivity in a small eutrophic lake. The chemical nature of the organic matter in the wal.er column, as determined by its source, had an important influence on its role in the coagulation and aggregation of inorganic colloids. During periods of high productivity, inorganic colloids were observed to be depleted at depths of maximum polysaccharide concentrations. Furthermore, in field experiments, depletion rates of colloidal particles were correlated to the presence of aquagenic biopolymers. In controlled laboratory experiments, the coagulation rate of colloidal montmorillonite was increased in the presence of 2.0 or 4.0 mg C liter-' of aquagenic biopolymers. Sedimentation also increased with increasing biopolymer concentrations (O-2.3 mg C liter-'). On the other hand, 0.1-6.3 mg C liter ' of pedogenically derived fulvic acids were shown to stabilize colloidal solutions, in agreement with previous studies.In the aquatic environment, the mobility and bioavailability of any given element depends on its chemical partitioning among the dissolved, colloidal, and particulate forms. It is known that the physicochemical conditions (e.g. temperature, ionic strength, pH, complexation by inorganic or organic ligands) that describe the aquatic system of interest play a direct role in the chemical speciation of toxic and vital compounds. Modifications in the physicochemistry of the media can also have an indirect influence on bioavailability through changes in the stability of the colloidal or particulate carriers.Both the mobility and biological availability of trace elements decrease upon their sorption to the particulate phase. The limit between dissolved and particulate matter is often arbitrarily and operationally defined by a 0.45-pm membrane. Based on their physicochemical role in the environment, colloids can be defined as the transition between the truly dissolved (5 1 nm) and the rapidly sedimenting (> 1O-4 m s-l) particulate species (21 pm). Colloids are an important carrier with the potential to remain in solution for long periods, sedimenting only after coagulation or aggregation. Owing to their large numbers, high surface area, and high adsorptive capacity, colloids or organic macromolecules and their aggregates are the predominant carriers of many toxic
AcknowledgmentsThis work was supported by grants from the Fond National Suisse (project Nos. 2000-037598.93/l and 2004-3568.95/l). K.J.W. acknowledges a postdoctoral fellowship from the National Science and Engineering Council of Canada. We acknowledge the technical assistance of J. Chorovcr, M. Depres, R. Fcrretti, and R. Giovannini. Thanks to J. Zhang, who offered constructive comments on an earlier version of the manuscript and provided insight into the coagulation process. The reviews of D. Kirchman, R Santschi, M. R. Twiss, and two anonymous reviewers are also greatly appreciated. Some preliminary experiments were performed with algal polysaccharides (S...