Vertical Transmission of Dengue Virus in Aedes aegypti Collected in Surabaya, Indonesia, during 2008^|^ndash;2011

Mulyatno, Kris Cahyo; Yamanaka, Akira; Yotopranoto, Subagyo; Konishi, Eiji

doi:10.7883/yoken.65.274

Cited by 30 publications

(22 citation statements)

References 9 publications

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“…In this report, we isolated a CHIKV strain from patients’ sera banked from the 2008–2009 Thai epidemics. We identified this virus is closely related to the Asian genotype, CHIK/SBY8/10 isolate originally found in patient’s blood in Indonesia [33]. To the best of our knowledge, no prior study addressed the characterization, replication, and release of the strain used in our study.…”

Section: Discussionmentioning

confidence: 90%

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Chalaem

Chusri

Fernandez

et al. 2016

Virol J

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BackgroundChikungunya virus (CHIKV) is a prevalent mosquito-borne pathogen that is emerging in many parts of the globe causing significant human morbidity. Here, we report the isolation and characterization of an infectious CHIKV from banked serum specimens of suspected patients from the 2009 epidemic in Thailand.MethodsStandard plaque assay was used for CHIKV isolation from the banked serum specimens. Isolated CHIKV was identified base on E1 structural gene sequence. Growth kinetic, infectivity, cell viability and cytokine gene expression throughout CHIKV infection in a permissive cell line, 293T cells, was performed using several approaches, including standard plaque assay, immunofluorescence assay, classical MTT assay, and quantitative real-time PCR. Two tailed Student’s t test was used for evaluation statistically significance between the mean values of the groups.ResultsBased on the E1 structural gene sequence and phylogenetic analysis, we identified the virus as the CHIK/SBY8/10 isolate from Indonesia. Assessment of the growth kinetics, cytopathic effects as well as its ability to induce cellular immune responses suggested that the currently isolated CHIK/SBY8/10 virus is relatively more virulent than a known CHIKV vaccine strain, which also induces more dramatic proinflammatory responses.ConclusionsOur studies further add to the infectivity of a less-studied yet infectious CHIKV isolate as well as underscored the importance and utility of 293T cells as an excellent cell culture model for studying viral growth, CHIKV-induced inflammatory cellular responses and cell death. Together, these studies provide novel information on the CHIKV biology, infectivity and virus-cell interaction, which would help develop novel interventions against the infection.

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Section: Discussionmentioning

confidence: 90%

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Chalaem

Chusri

Fernandez

et al. 2016

Virol J

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“…A full assessment of the 120 references resulted in the exclusion of 53 references due to the following: full texts were unavailable, and the available abstracts did not include adequate information to satisfy our outcome, the full text did not include adequate or complete serotype data, and nonstandard methods were used for serotyping. This resulted in 67 eligible studies, of which 13 references were from national journals . An additional 11 studies were included from reference list .…”

Section: Resultsmentioning

confidence: 99%

“…In total, 78 eligible studies were included for DENV serotype analysis . Of all the eligible studies, four studies were conducted based on mosquito isolates while the remaining studies assessed DENV isolates from human samples . Sixty two studies investigated dengue cases among local inhabitants of Indonesia,…”

Section: Resultsmentioning

confidence: 99%

“…Of all the eligible studies, four studies were conducted based on mosquito isolates while the remaining studies assessed DENV isolates from human samples . Sixty two studies investigated dengue cases among local inhabitants of Indonesia, and the remaining 16 reported traveller‐associated dengue cases from other countries …”

Section: Resultsmentioning

confidence: 99%

“…A comprehensive study conducted in Surabaya found that the serotype shift from DENV‐2 to DENV‐1 occurred in late 2008 . To elucidate this, we analysed all available serotype data from Surabaya and confirmed DENV‐1 became the predominant serotype in this region between 2008 and 2009 and continued to circulate until 2013 before being replaced by DENV‐2 in 2014 (Table ). The timing of the serotype shifts observed in Surabaya may not, however, represent all regions of Indonesia.…”

Section: Resultsmentioning

confidence: 99%

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Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

Harapan

Michie

Yohan

et al. 2019

Reviews in Medical Virology

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Although epidemiological and molecular epidemiological (serotype, genotype, and lineage information) data are available for several major cities in Indonesia, there is yet to be a comprehensive national study of dengue in Indonesia over time. This study was conducted to provide a comprehensive epidemiology of circulating dengue viruses (DENV) in Indonesia between 1973 and 2016. This was conducted through a systematic review of the literature and phylogenetic analysis of available DENV sequences. Available data from National Disease Surveillance System have indicated an increasing trend of dengue incidence in Indonesia over the past 50 years. Incidence rates appear to be cyclic, peaking approximately every 6 to 8 years. In contrast, the case fatality rate has decreased approximately by half with each decade since 1980.Over this 50-year time span, serotype shifts, genotype displacement within DENV-1 and DENV-2, and introduction of DENV-1 and DENV-3 genotype from other countries occurred. These events were associated with increased incidence of dengue cases. Our study also provides a valuable national snapshot of DENV genetic diversity in Indonesia that may contribute to development of more effective dengue vaccine compositions for the region.

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Development of alpha-cypermethrin resistance and its effect on biological parameters of yellow fever mosquito, Aedes aegypti (L.) (Diptera: Culicidae)

Shafiq,

Abubakar,

Riaz

et al. 2023

Parasitol Res

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Vertical Transmission of Dengue Virus in Aedes aegypti Collected in Surabaya, Indonesia, during 2008^|^ndash;2011

Cited by 30 publications

References 9 publications

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Characterization of a Chikungunya virus strain isolated from banked patients’ sera

Dengue viruses circulating in Indonesia: A systematic review and phylogenetic analysis of data from five decades

Development of alpha-cypermethrin resistance and its effect on biological parameters of yellow fever mosquito, Aedes aegypti (L.) (Diptera: Culicidae)

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