Vertical flow-based paper immunosensor for rapid electrochemical and colorimetric detection of influenza virus using a different pore size sample pad

Bhardwaj, Jyoti; Sharma, Abhinav; Jang, Jaesung

doi:10.1016/j.bios.2018.10.008

Cited by 99 publications

(48 citation statements)

References 19 publications

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“…Because the paper has strong adhesion ability after treatment, it can attach more antigens than the untreated paper, which is the first amplification. Then, AuNps conjugate with several copies of Ab1 and Ab1′, which is the second amplification [43]. Finally, GO sheets conjugate more HRP than Ab2, resulting in an increase in the ratio of HRP to Ab2, which is the third amplification (shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Lü

Zhang²,

Wang

et al. 2019

BMC Chemistry

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A newly modified paper-based enzyme-linked immunosorbent assay (P-ELISA) was established by immobilizing more proteins on the paper surface through an atom transfer radical polymerization (ATRP) reaction. In addition, introducing graphene oxide (GO) sheets, Au nanoparticles (AuNps) and two primary antibodies (Ab1s) led to signal amplification and cost reduction. Electronic supplementary material The online version of this article (10.1186/s13065-019-0622-7) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Lü

Zhang²,

Wang

et al. 2019

BMC Chemistry

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“…HRP was also proposed for developing EC-LFIAs systems, by exploiting its electroactive features. As an example, a vertical-flow-based LFIA was developed for the simultaneous EC and colorimetric detection of influenza H1N1 viruses (Figure 2) [65]. In this HRP was also proposed for developing EC-LFIAs systems, by exploiting its electroactive features.…”

Section: Enzyme-labeled Conjugates In Lfiamentioning

confidence: 99%

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Calabria

Calabretta

Zangheri

et al. 2021

Sensors

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Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

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“…The variety of different electrodes, transducers, and detection techniques for Influenza virus detection, encouraged the development of innovative systems. Jang was the first to develop a low-cost, miniaturized paper-based [137] , followed by a flow-based paper [138] immunosensor for electrochemical and colorimetric detection of Influenza H1N1 virus. The evolution from simple paper to flow-paper promoted a decrease in the limit of detection from 113 PFU.mL −1 to 3.3 PFU.mL −1 (electrochemical) and 1.34 PFU.mL −1 (colorimetric).…”

Section: Viral Respiratory Biosensorsmentioning

confidence: 99%

Biosensors for the detection of respiratory viruses: A review

Cordeiro²,

et al. 2020

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Highlights A review containing the newest contributions of biosensors for respiratory viruses’ detection is presented. Influenza virus, coronavirus and respiratory syncytial virus were extensively analyzed. Tables containing biosensors information regarding the detection of the viruses are presented. Discussion based on the new trends and authors accomplishments are applied. SARS-CoV-2 biosensors already published were included and analyzed.

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Vertical flow-based paper immunosensor for rapid electrochemical and colorimetric detection of influenza virus using a different pore size sample pad

Cited by 99 publications

References 19 publications

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Atom transfer radical polymer-modified paper for improvement in protein fixation in paper-based ELISA

Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Biosensors for the detection of respiratory viruses: A review

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