Versican silencing in BeWo cells and its implication in gestational trophoblastic diseases

Pires, Keyla; Sun, Sue Yazaki; Gonçalves, Camilla M.; Santos, Jademilson Celestino dos; Tenório, Luciana Lucena; Botelho, Rayane Martins; Silva, Ana Lucia M.; Oliveira, H.G.S.; Pendeloski, Karen Priscila T; Daher, Sílvia; Borbely, Karen Steponavicius Cruz; Borbely, Alexandre Urban

doi:10.1007/s00418-018-1739-9

Cited by 7 publications

(5 citation statements)

References 36 publications

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“…Three pathways were shared by both gene lists as potentially the most important ones, namely the ‘PI3K-Akt signaling pathway’, ‘steroid hormone biosynthesis’, and ‘proteoglycans in cancer’. Proteoglycans may interact with the ‘PI3K-Akt signaling pathway’ to affect trophoblast differentiation and cell migration/invasion [ 65 , 68 ]. Although the ‘PI3K-Akt signaling pathway’ pathway was also impacted in moles, several genes in this pathway were specifically affected only in choriocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease

et al. 2021

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Gestational trophoblastic diseases (GTDs) have not been investigated for their epigenetic marks and consequent transcriptomic changes. Here, we analyzed genome-wide DNA methylation and transcriptome data to reveal the epigenetic basis of disease pathways that may lead to benign or malignant GTDs. RNA-Seq, mRNA microarray, and Human Methylation 450 BeadChip data from complete moles and choriocarcinoma cells were bioinformatically analyzed. Paraffin-embedded tissues from complete moles and control placentas were used for tissue microarray construction, DNMT3B immunostaining and immunoscoring. We found that DNA methylation increases with disease severity in GTDs. Differentially expressed genes are mainly upregulated in moles while predominantly downregulated in choriocarcinoma. DNA methylation principally influences the gene expression of villous trophoblast differentiation-related or predominantly placenta-expressed genes in moles and choriocarcinoma cells. Affected genes in these subsets shared focal adhesion and actin cytoskeleton pathways in moles and choriocarcinoma. In moles, cell cycle and differentiation regulatory pathways, essential for trophoblast/placental development, were enriched. In choriocarcinoma cells, hormone biosynthetic, extracellular matrix-related, hypoxic gene regulatory, and differentiation-related signaling pathways were enriched. In moles, we found slight upregulation of DNMT3B protein, a developmentally important de novo DNA methylase, which is strongly overexpressed in choriocarcinoma cells that may partly be responsible for the large DNA methylation differences. Our findings provide new insights into the shared and disparate molecular pathways of disease in GTDs and may help in designing new diagnostic and therapeutic tools.

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Section: Discussionmentioning

confidence: 99%

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease

et al. 2021

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“…However, patients who experienced spontaneous remission had hydatidiform moles with higher rates of apoptosis than patients who advanced to GTN, which might have been a sign of malignant change. The same could be said for versican expression, which is eliminated in choriocarcinoma and invasive moles but is highly expressed in healthy tissues and hydatidiform moles with spontaneous remission [14]. Its absence could also serve as a potential marker to anticipate malignant transformation.…”

Section: Biomarker For Diagnosismentioning

confidence: 95%

A Review of Current Management of Gestational Trophoblastic Disease

Mahendra

Brahmantara²,

Setiawan

2022

EJMED

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A set of conditions connected to pregnancy and caused by trophoblast cells are known as gestational trophoblastic diseases. There are benign and malignant cancers, including invasive moles, choriocarcinomas, placental site trophoblastic tumors (PSTT), and epithelioid trophoblastic tumors (ETT). These cancers are classified as gestational trophoblastic neoplasms (GTN), which are less common but more serious. Most often, monitoring serum human chorionic gonadotropin (hCG) with histological confirmation is used to diagnose GTN. As a differential diagnosis, certain tissue biomarkers have grown in popularity. This has produced more accurate results and various treatment regimens and prognoses for each GTN. The World Health Organization's prognostic score system and the International Federation of Gynecology and Obstetrics anatomical staging system are the foundations for the treatment. Suppose the proper diagnosis is made and the instructions are followed. In that case, choriocarcinoma and invasive mole cases can be cured in 98 percent of instances, whereas PSTT and ETT still have only moderate success rates. The increased understanding of GTN and its characteristics enables medical professionals to rapidly make a differential diagnosis and select the appropriate treatment option, thus improving the overall survival of affected women. Nevertheless, epidemiological data collection and knowledge advancement through basic and translational research are crucial to address issues regarding GTN pathophysiology, their origins, and cellular behaviour.

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“…This study is in accordance with a study conducted before where the incidence of GTD is most commonly found at the age of 31 years. Another study found that the incidence of GTD was mostly found in the 20-30 year age range, which was 54,8% [5], [6].…”

Section: A Characteristics Of Gtd Cases By Agementioning

confidence: 96%

“…This study is in accordance with a study conducted before where the incidence of GTD was most commonly found in parity 2. Another study found that the incidence of GTD was most commonly found in parity 2 [5], [6].…”

Section: B Characteristics Of Gtd Cases Based On Paritymentioning

confidence: 98%

Characteristics of Gestational Trophoblastic Disease at Prof. Dr. I.G.N.G. Ngoerah Hospital, Denpasar from 1st January 2020 to 31st December 2021

Budiana,

Megadhana,

Putra Wiradnyana

et al. 2023

EJMED

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GTD is a rare abnormal condition of pregnancy, where the condition occurs when trophoblast cells proliferate uncontrollably so that it develops into an abnormal pregnancy. Abnormal pregnancy can lead to various negative effects such as vaginal bleeding, shortness of breath, and others. The high incidence of GTD is still not well documented to date. It is important to manage GTD appropriately to minimize acute complications and identify gestational trophoblastic tumor promptly. In this study, studyers took data from the oncogynecology register book regarding GTD and then presented it as descriptive data so that descriptive data analysis was carried out in the form of tables and narratives. The results of this study showed that GTD was highest in the age range of 31-35 years, parity 2, clinical symptoms of vaginal bleeding, previous pregnancy history with hydatidiform mole, gestational interval of 4 months or less, β-HCG level more than 100.000 mIU/ml, no metastases, histopathology of complete hydatidiform mole, stage I GTD, and receiving metrotrexate chemotherapy.

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Versican silencing in BeWo cells and its implication in gestational trophoblastic diseases

Cited by 7 publications

References 36 publications

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease

Epigenetic Dysregulation of Trophoblastic Gene Expression in Gestational Trophoblastic Disease

A Review of Current Management of Gestational Trophoblastic Disease

Characteristics of Gestational Trophoblastic Disease at Prof. Dr. I.G.N.G. Ngoerah Hospital, Denpasar from 1st January 2020 to 31st December 2021

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