Versatile Two-Phase Solvent System for Anthraquinone Prefractionation by High Speed Countercurrent Chromatography

Yua, LI; Chen, X. X.; Ai, Ping; Zi, Min; Wu, Peng; Li, Z. Y.

doi:10.1081/jlc-100107349

Cited by 10 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Os sistemas de solventes usados foram misturas de clorofórmio/metanol/água 4/ X/2, onde X variou entre 3 e 4 para cada um dos extratos. Como as antraquinonas possuem uma grande variação em termos de polaridade, o sistema escolhido apresentou resultados excelentes, já que o metanol pode variar, simultaneamente, a seletividade tanto da fase superior quanto da fase inferior, podendo então ser modulada através da variação na concentração desse solvente no sistema (YUA et al, 2001).…”

Section: Separação De Antraquinonasunclassified

Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal

Leitão

2005

Rev Fitos

View full text Add to dashboard Cite

A técnica da cromatografia contracorrente é aqui apresentada como uma ferramenta eficiente, rápida e econômica de obtenção de padrões de origem vegetal com alto grau de pureza. São discutidos alguns aspectos da escolha dos sistemas de solventes adequados além de exemplos de isolamento e purificação de substâncias naturais de importância farmacêutica tais como flavonóides de Ginkgo biloba, saponinas de Centella asiatica e Ginseng, antraquinonas, etc.

show abstract

Section: Separação De Antraquinonasunclassified

Uso da Cromatografia Contracorrente na obtenção de padrões de origem Vegetal

Leitão

2005

Rev Fitos

View full text Add to dashboard Cite

show abstract

“…For instance, target‐guided separation of potential antioxidants, including six hydroxyanthraquinones and two glycosides, from Cassiae Semen by conventional high speed CCC using complexation and extrusion elution mode was reported, which provided a rapid and effective technique to screen antioxidants from natural products . To establish a method for general prefraction of anthraquinone from five traditional Chinese medicines, a biphasic solvent system containing chloroform was used for separation by CCC .…”

Section: Introductionmentioning

confidence: 99%

pH‐zone‐refining elution–extrusion countercurrent chromatography: Separation of hydroxyanthraquinones from Cassiae semen

et al. 2017

J of Separation Science

View full text Add to dashboard Cite

Seven hydroxyanthraquinones were successfully separated from the traditional Chinese medicinal herb Cassiae semen by conventional and pH-zone-refining countercurrent chromatography with an environmentally friendly biphasic solvent system, in which elution-extrusion mode was investigated for pH-zone-refining countercurrent chromatography for the first time. A two-phase solvent system composed of n-hexane/ethyl acetate/ethanol/water (5:3:4:4, v/v/v/v) was used for the conventional countercurrent chromatography while the same system with a different volume ratio n-hexane/ethyl acetate/ethanol/water (3:5:2:6, v/v/v/v) was used for pH-zone-refining countercurrent chromatography, in which 20 mmol/L of trifluoroacetic acid was added in the organic phase as a retainer and 15 mmol/L of ammonia was added to the aqueous phase as an eluter. A 400 mg crude sample could be well separated by pH-zone-refining countercurrent chromatography, yielding 53 mg of aurantio-obtusin, 40 mg of chryso-obtusin, 18 mg of obtusin, 24 mg of obtusifolin, 10 mg of emodin, and 105 mg of the mixture of chrysophanol and physcion with a purity of over 95.8, 95.7, 96.9, 93.5, 97.4, 77.1, and 19.8%, as determined by high-performance liquid chromatography. Furthermore, the difference in elution sequence between conventional and pH-zone-refining mode was observed and discussed.

show abstract