Versatile Au@Ru nanocomposites for the rapid detection of Salmonella typhimurium and photothermal sterilization

Ji, Li; Zhang, Leiheng; Yang, Huiyi; Liang, Shan; Pan, Junkang; Zou, Yikui; Li, Shijia; Li, Qinglan; Zhao, Suqing

doi:10.1016/j.jcis.2022.04.028

Cited by 17 publications

(8 citation statements)

References 50 publications

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“…2H . It could be seen that the hydrodynamic diameter produced an obvious increase from 76 to 150 nm, and the zeta potential changed from − 66 to − 16 mV, which demonstrated the antibody was successfully coupled with the NPs [ 37 – 39 ]. Furthermore, Au-Fe 3 O 4 and Au-Fe 3 O 4 -Ab were, respectively, loaded on the LFIA strips, and as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, compared with carbon nanoparticles, quantum dots, up-converting materials, etc. [ 39 , 41 – 44 ], Au-Fe 3 O 4 NPs enabled enrichment and purification of target bacteria from complex matrix, thus achieving direct application to unprocessed whole milk. Besides, Au-Fe 3 O 4 NPs could provide photothermal signal, facilitating quantitative detection with a portable infrared thermal camera, which was more sensitive than color observing and more cost-effective than fluorescence or magnetic signal determination [ 27 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Colorimetric and photothermal dual-mode lateral flow immunoassay based on Au-Fe3O4 multifunctional nanoparticles for detection of Salmonella typhimurium

et al. 2023

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Au-Fe 3 O 4 multifunctional nanoparticles (NPs) were synthesized and integrated with lateral flow immunoassay (LFIA) for dual-mode detection of Salmonella typhimurium . The Au-Fe 3 O 4 NPs not only combined excellent local surface plasmon resonance characteristics and superparamagnetic properties, but also exhibited good photothermal effect. In the detection, antibody-conjugated Au-Fe 3 O 4 NPs first captured S. typhimurium from complex matrix, which was then loaded on the LFIA strip and trapped by the T-line. By observing the color bands with the naked eyes, qualitative detection was performed free of instrument. By measuring the photothermal signal, quantification was achieved with a portable infrared thermal camera. The introduction of magnetic separation achieved the enrichment and purification of target bacteria, thus enhancing the detection sensitivity and reducing interference. This dual-mode LFIA achieved a visual detection limit of 5 × 10 5 CFU/mL and a photothermal detection limit of 5 × 10 4 CFU/mL. Compared with traditional Au-based LFIA, this dual-mode LFIA increased the detection sensitivity by 2 orders of magnitude and could be directly applied to unprocessed milk sample. Besides, this dual-mode LFIA showed good reproducibility and specificity. The intra-assay and inter-assay variation coefficients were 3.0% and 7.9%, and with this dual-mode LFIA, other bacteria hardly produced distinguishable signals. Thus, the Au-Fe 3 O 4 NPs-based LFIA has potential to increase the efficiency of pandemic prevention and control. Graphical Abstract Au-Fe 3 O 4 nanoparticle proved to be a promising alternative reporter for LFIA, achieving multifunctions: target purification, target enrichment, visual qualitation, and instrumental quantification, which improved the limitations of traditional LFIA. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-023-05645-x.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Colorimetric and photothermal dual-mode lateral flow immunoassay based on Au-Fe3O4 multifunctional nanoparticles for detection of Salmonella typhimurium

et al. 2023

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“…The colorimetric lateral flow immunoassay (cLFIA), as one of the most prominent methods of point-of-care testing (POCT) for monitoring analytes, has played an increasingly key role in medical diagnostics, environmental monitoring, and food analysis because of its cost-effectiveness, user-friendliness, rapidity, excellent selectivity, and wide applicability. − Generally, the analytical performance of cLFIA is highly dependent on the optical signal tracer of monoclonal antibodies (SR-mAbs), because they are generators of the reading signals in cLFIA sensing systems. − Recently, the exploitation of dyestuffs to serve as SR-mAbs in the cLFIA has drawn plentiful interest due to its simple steps, cheap chemical reagents, time-saving steps, satisfactory performance, and reduction of the discrepancy between batches of materials synthesis. − The most significant precondition for the manufacture of a dyestuff-based cLFIA is the successful staining of the mAbs by dyestuffs . However, the current development depends too much on trial-and-error strategies owing to the lack of a universal description of the dyestuffs as the SR of cLFIA.…”

Section: Introductionmentioning

confidence: 99%

“Potential Scalpel”: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results

Jia

Shi

et al. 2023

Anal. Chem.

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It is of great importance to overcome potential incompatibility problems between dyestuffs and antibodies (mAbs) for extensive commercial application of a dyestuff-chemistry-based ultrafast colorimetric lateral flow immunoassay (cLFIA). Herein, inspired by traditional staining technologies, a basic dyestuff gallocyanine (GC)-assisted biogenic "potential scalpel"-based cLFIA (GC-ABPS-based cLFIA) by employing clenbuterol (CLE) as proof-of-concept was proposed to solve a high degree of incompatibility between the same potential dyestuffs and mAbs. Goat antimouse immunoglobulin (Ab 2 ) could serve as the "potential scalpel" to form the positive potential value biomolecular network self-assemblers (BNSA) with anti-CLE mAbs (Ab CLE ) by noncovalent force. The cLFIA completed the entire detection process from de novo to detection results within 30 min thanks to the easy availability and ideal marking efficiency (≤1 min, saving 0.4−10 h) of GC. Encouragingly, the proposed ultrafast GC-ABPSbased cLFIA has also exhibited high sensitivity (0.411 ng mL −1 ) and low cost (300 times) compared with other cLFIAs. Also, the feasibility of the proposed cLFIA was demonstrated by detecting CLE in beef, pork ham, and skim milk. Finally, the proposed GC-ABPS-based cLFIA has broadened the application range of dyestuffs and provided an effective reference strategy for the application of dyestuffs in food safety monitoring.

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“…As a common public health security hazard, food-borne pathogens have always been a foremost threat to human health. 24,25 According to the report, more than 90% food poisoning diseases are caused by pathogenic bacteria. 26 Therefore, there is an urgent demand to develop a simple, rapid, and portable detection method for food-borne pathogens.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic chip-based multivalent DNA walker amplification biosensor for the simultaneous detection of multiple food-borne pathogens

Wang

Jia

et al. 2023

Analyst

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Versatile Au@Ru nanocomposites for the rapid detection of Salmonella typhimurium and photothermal sterilization

Cited by 17 publications

References 50 publications

Colorimetric and photothermal dual-mode lateral flow immunoassay based on Au-Fe3O4 multifunctional nanoparticles for detection of Salmonella typhimurium

Colorimetric and photothermal dual-mode lateral flow immunoassay based on Au-Fe3O4 multifunctional nanoparticles for detection of Salmonella typhimurium

“Potential Scalpel”: A Bioassisted Ultrafast Staining Lateral Flow Immunoassay from De Novo to Results

A microfluidic chip-based multivalent DNA walker amplification biosensor for the simultaneous detection of multiple food-borne pathogens

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