A microfluidic chip-based multivalent DNA walker amplification biosensor for the simultaneous detection of multiple food-borne pathogens

Abstract

The rapid, simultaneous, sensitive detection of the targets has important application prospects for disease diagnosis and biomedical researches. However, in practical applications, the content of the targets is usually very...

Design and bioanalytical applications of stochastic DNA walkers

“…58,59 It is also possible to enhance the processivity of DNA walkers using advanced microfluidic systems. 68 Towards bioanalytical applications, numerous efforts have also been made to integrate varying signal readout systems for DNA walkers to enhance the portability and multiplexity of DNA walker-based assays. 69,70 Another interesting future direction is to integrate synthetic DNA walkers with living cells to achieve functions beyond sensing and imaging.…”

The rate-limiting procedure of 3D DNA walkers and their applications in tandem technology

“…It has been found that using biogenic material as a support can effectively avoid a strong adsorption effect on DNA and reduce steric hindrance during the DNA walking process. 41,42 Besides, universal 3D walkers that are based on hard nanoparticles hinder the relative motion between the tracks and arms, thereby reducing walking efficiency. Moreover, static anchoring of tracks on 3D landscapes is mandatory, which further complicates the construction process.…”

Advancing in situ single-cell microbiological analysis through a microwell droplet array with a gradual open sidewall

“…Patients with pre-existing chronic conditions or weakened immune systems are particularly vulnerable to infections caused by pathogenic bacteria. 1,2 The development of precise and effective pathogen detection technologies is essential for the successful diagnosis and treatment of infectious illnesses since existing detection methods are sometimes time-consuming and difficult. 3,4 The single-cell analysis (SCA) technique based on microfluidics provides detailed information about intracellular substances and biochemical reactions.…”