Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.)

Abstract

A novel endophytic filamentous bacterium strain A-T 7972, was isolated from wild orchid Grosourdya appendiculata (Blume) Rchb.f. collected in Thailand. The bacterium developed single non-motile spores with warty surface on substrate mycelia. The taxonomic position was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 7972 belonged to the genus Verrucosispora and shared the highest sequence similarity with V. lutea YIM 013 (98.71 %) and V. gifhornensis DSM 44337 (98.53 %). The values of DNA-DNA relatedness that distinguished this novel strain from its closest species were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were mannose, ribose, glucose and xylose. The predominant menaquinone was MK-9(H4). The predominant fatty acids were branched fatty acids iso-C15 : 0 and iso-C16 : 0. The diagnostic phospholipids profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 70.5 mol%. Based on its phenotypic, chemotaxonomic and genotypic characteristics, the new isolate A-T 7972 (=BCC 50981=TBRC 6031=NBRC 112512) is proposed to be the type strain of novel species, Verrucosispora endophytica sp. nov.

Diving Into Reef Ecosystems for Land-Agriculture Solutions: Coral Microbiota Can Alleviate Salt Stress During Germination and Photosynthesis in Terrestrial Plants

“…We also found that the interaction between N. attenuata and S. arenicola resembled that of plant-endophytic symbiosis ( Figure 5A). Other actinobacteria (i.e., Micromonospora and Verrucosispora) from the same family of the coral symbiont Salinispora have been isolated from the root nodules of terrestrial and marine plants where they presented endophytic interactions and conferred advantages to their plant hosts (Figure 2; Li et al, 2013;Ngaemthao et al, 2017;Kuncharoen et al, 2018). Bioinformatic analysis suggests that Salinispora spp.…”

Diversity and Colonization of Endophytic Actinomycetes in some Medicinal Plants: Review

“…Morphological characteristics are observed by light and scanning electron microscopes and growth at different temperatures (5-60°C) and different pH values are recorded. 81 Some physiological and biochemical characteristics like utilization of different carbon and nitrogen sources by the actinomycetes are important for identification. 82 Cell chemistry was determined by detecting diagnostic sugars in whole-cell hydrolysates, amino acids in the cell wall and cell total content of fatty acids and phospholipids.…”

Micromonospora tarapacensis sp. nov., a bacterium isolated from a hypersaline lake

“…However, the neighbour-joining tree (Fig. 2) indicated that Llam7 T formed a distinct phylogenetic line with low bootstrap values next to Verrucosispora endophytica A-T 7972 T (98.04 %) [22] and Micromonospora deserti 13K206 T (98.61 %) [23]. It appears more closely related to species of the genus Salinispora species than to the bulk of species of the genus Micromonospora.…”

“…The complete 16S rRNA gene sequence of Llam7 T showed high similarity values with members of the family Micromonosporaceae in the range of 98.2–99.1 %, showing the highest 16S rRNA gene sequence similarity values with Micromonospora craniellae LHW63014 T (99.10 %) [16], Micromonospora craterilacus NA12 T (98.96 %) [17], Micromonospora zingiberis PLAI 1-1 T (98.86 %) [18], Micromonospora phaseoli CGMCC 4.7038 T (98.75 %) [19], Salinispora pacifica CNR-114 T (98.68 %) [20] and Salinispora goodfellowii CNY-666 T (98.68 %) [21]. However, the neighbour-joining tree () indicated that Llam7 T formed a distinct phylogenetic line with low bootstrap values next to Verrucosispora endophytica A-T 7972 T (98.04 %) [22] and Micromonospora deserti 13K206 T (98.61 %) [23]. It appears more closely related to species of the genus Salinispora species than to the bulk of species of the genus Micromonospora .…”