Verlauf von Vitamin-B6-Gehalten wachsender Ratten bei Vitamin-B6-freier Ernährung

Roth‐Maier, Dora A.; Kirchgeßner, M.

doi:10.1007/bf02023693

Cited by 7 publications

(1 citation statement)

References 8 publications

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“…A dose-response-relationship between the dietary vitamin B 6 intake and the vitamin B 6 status has been determined in several studies with growing, pregnant and lactating rats (Benedikt et al, 1996c, Reithmayer et al, 1985aand b, Roth-Maier and Kirchgeßner, 1981and 1989. However, there are less results to date about the effect of various dietary vitamin B 6 supplies during gestation on the vitamin B 6 level of lactating rats.…”

Section: Discussionmentioning

confidence: 95%

Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams

Roth‐Maier

Benedikt

Stangl

et al. 1996

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The present investigation was designed to determine the vitamin B6 concentration in liver and carcass of rat dams fed various dietary vitamin B6 levels during gestation and lactation. Therefore, gravid female rats received 0.6, 3, 6, 18 or 180 mg vitamin B6 per kg diet. After parturition each group was divided into 2 groups of 8 dams each, which received then a diet with 3, respectively 6 mg/kg vitamin B6 during lactation. At the end of the experiment at day 14 of lactation weight gain and food consumption as well as liver and carcass weights did not differ within the groups. The present data clearly show that both in liver and carcass of lactating rats, there exists only a slight dose-response-relationship between the dietary vitamin B6 intake and the vitamin B6 concentration in body tissue. Moreover, liver reflects the various vitamin B6 supplies during gestation and lactation somewhat better than carcass. The distribution of the vitamins did not differ among the groups and was therefore independent of the vitamin B6 supply. The present findings, especially regarding the liver, elucidate, that an adequate vitamin B6 supply during lactation can not compensate for a lack of vitamin B6 during gestation and vice versa a high dose of vitamin B6 during gestation did not completely protect against a suboptimal vitamin B6 during lactation.

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Section: Discussionmentioning

confidence: 95%

Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams

Roth‐Maier

Benedikt

Stangl

et al. 1996

Archiv für Tierernaehrung

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Dietary thiamin supply during lactation influences thiamin status in lactating rats and their offspring and the thiamin level in milk

et al. 1997

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This study was conducted to examine the effect of dietary thiamin, ranging from deficient to excessive supplies, on thiamin status of lactating rats and their offspring, and the thiamin level in milk. Therefore, after parturition, rat dams were divided into eight groups of 10 each, and were fed diets with 0, 2, 4, 6, 7, 40, 350 and 3500 mg/kg thiamin over a total of 13 days during lactation. Milk for determining the thiamin concentration was obtained from day 6 and 13 of lactation. At day 14 of lactation rat dams and their offspring were used to ascertain the thiamin status including transketolase activity of blood, liver and brain, and thiamin concentration in body. Thiamin supplies ranging from deficient to excessive dietary concentrations influenced both the thiamin levels of the lactating dams and their offspring within 13 days. Lactating rat dams fed a thiamin-free diet and their offspring were classified as thiamin-deficient on the basis of growth retardation and a lower activity of transketolase in blood, liver and brain. Within these variables transketolase in blood has been shown to be most sensitive, and reached a plateau feeding 6 mg/kg thiamin. The concentration of thiamin in milk ranged between 0.1 and 19 mg/kg. The findings also show that dietary thiamin had the strongest effect on thiamin in milk obtained from day 6 and 13 of lactation, and a deficient or suboptimal supply with thiamin was therefore not compensated for an intensified transfer of reserved body thiamin into milk. Also thiamin levels in tissues and carcass, which did not show any clear-cut saturation characteristic, increased with increasing dietary thiamin, and this dose-dependence was more marked in blood and liver than in carcass.

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Untersuchungen zum Einfluß einer unterschiedlichen Vitamin-B6-Versorgung auf den Vitamin-B6-Status (Pyridoxin, Pyridoxal und Pyridoxamin) der Leber und des Körpers laktierender Ratten

Benedikt

Kirchgeßner

1996

Z Ernährungswiss

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Eighty female Sprague-Dawley rats were fed a semisynthetic diet during gravidity which was supplemented with 5 mg vitamin B6 per kg diet. The daily food intake was 14 g. During the following lactation the rats were assigned to one of 10 vitamin B6 treatment groups (0, 3, 6, 9, 12, 15, 18, 36, 360 and 3,600 mg per kg diet). The feed was given ad libitum. At day 14 of lactation the rats were decapitated. Parameters for determination of the vitamin B6 status were concentration of pyridoxine, pyridoxal and pyridoxamine in liver and body analyzed by using HPLC. Body was defined without the gastroenteral tract that was divided into carcass (extrahepatic compartments without liver) and total body (extrahepatic compartments plus liver). The mean weight of liver was 13 g with a dry mass of 33%; there was no difference between the treatment groups. The vitamin B6 concentration was lowest in rats fed 0 mg vitamin B6/kg diet (5 micrograms/g fresh matter, FM) and highest in the rats fed 3600 mg vitamin B6/kg diet (10.9 micrograms/g FM). The total vitamin B6 consisted on the average of 38% pyridoxal and 62% pyridoxamine. This was only changed significantly at the highest supplementation level, where 20% pyridoxine were detected instead of pyridoxamine. The mean weight of carcass averaged 212 g at a dry matter content of 31%. The vitamin B6 concentration ranged in the treatment groups from 0 mg to 360 mg vitamin B6/kg diet between 2.1 micrograms/g FM and 2.8 micrograms/g FM. It was highest in the 3600 mg vitamin B6 treatment group at 7.5 micrograms/g FM. The total vitamin B6 consisted of 63% pyridoxal and 37% pyridoxamine. It was only significantly affected in the 3600 mg vitamin B6 treatment group, where also pyridoxine could be found in the amount of 56%. The results indicate that alimentary vitamin B6 supply had more influence on liver vitamin B6 concentration than on carcass concentration. Total body concentration is very similar carcass concentration, as 95% of vitamin B6 is located there. The suitability of the parameters by the evaluation of the vitamin B6 requirement was confirmed the comparison of two statistical methods. It is concluded that a vitamin B6 supply of 5 to 6 mg/kg diet is necessary to meet the requirements during lactation.

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Verlauf von Vitamin-B6-Gehalten wachsender Ratten bei Vitamin-B6-freier Ernährung

Cited by 7 publications

References 8 publications

Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams

Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams

Dietary thiamin supply during lactation influences thiamin status in lactating rats and their offspring and the thiamin level in milk

Untersuchungen zum Einfluß einer unterschiedlichen Vitamin-B6-Versorgung auf den Vitamin-B6-Status (Pyridoxin, Pyridoxal und Pyridoxamin) der Leber und des Körpers laktierender Ratten

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Verlauf von Vitamin-B6-Gehalten wachsender Ratten bei Vitamin-B6-freier Ernährung

Cited by 7 publications

References 8 publications

Effect of feeding various dietary vitamin B6concentrations during gestation and lactation on vitamin B6level in liver and carcass of rat dams

Effect of feeding various dietary vitamin B6concentrations during gestation and lactation on vitamin B6level in liver and carcass of rat dams

Dietary thiamin supply during lactation influences thiamin status in lactating rats and their offspring and the thiamin level in milk

Untersuchungen zum Einfluß einer unterschiedlichen Vitamin-B6-Versorgung auf den Vitamin-B6-Status (Pyridoxin, Pyridoxal und Pyridoxamin) der Leber und des Körpers laktierender Ratten

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Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams

Effect of feeding various dietary vitamin B₆concentrations during gestation and lactation on vitamin B₆level in liver and carcass of rat dams