Verification of Frequency in Species of Nontuberculous Mycobacteria in Kermanshah Drinking Water Supplies Using the PCR-Sequencing Method

Mohajeri, Parviz; Yazdani, Laya; Shahraki, Abdolrazagh Hashemi; Alvandi, Amirhooshang; Atashi, Sara Naybandi; Farahani, Abbas; Almasi, Ali; Rezaei, Mansour

doi:10.1089/mdr.2016.0064

Cited by 16 publications

(17 citation statements)

References 19 publications

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“…1 Our data show that 16S rRNA gene sequencing, as a single identification target, cannot be used an efficient method for the identification of closely-related mycobacterium species such as M. abscessus, M. bolletii or M. massiliense also pathogenic M. kansasii was easily differentiated from nonpathogenic M. gastri by rpoB analysis; this differentiation cannot be achieved by using 16S rRNA gene sequences. 18 In contrast to our study, Azadi et al 19 reported that the three most prevalent species of NTM in hospital water system were M. lentiflavum (28.2%), M. paragordonae (21.1%), and M. fredriksbergense (9.8%). In America and most parts of Europe, M. avium complex was the most common of NTM, in this regard in a study conducted by Molicotti in Sassari (Italy), 6 M. avium and M. gordonae had the highest frequency among NTM.…”

Section: Discussioncontrasting

confidence: 99%

“…17 In contrast with our previous study on frequency in species of NTM in Kermanshah drinking water supplies, we found following NTM isolates; M. goodii, M. aurum, and M. gastri were the most prevalent species (11.5%) and we not found M. simiae and M. fortuitum in drinking water supplies. 18 In contrast to our study, Azadi et al 19 reported that the three most prevalent species of NTM in hospital water system were M. lentiflavum (28.2%), M. paragordonae (21.1%), and M. fredriksbergense (9.8%). Another study of 14 provinces in Iran by Velayati and his colleagues showed that M. simiae is the most common species.…”

Section: Discussioncontrasting

confidence: 99%

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Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens byPCR‐sequencing method inWest ofIran

Dastranj

Farahani

Shahraki

et al. 2017

Clinical Respiratory J

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The results of our study showed that the patients were infected by a wide range of atypical mycobacteria. It was concluded that 16S rRNA gene sequencing coupled with rpoB marker is a high discriminatory power in identification of NTM. The presence of various species in clinical samples in Iran emphasizes the use of molecular method like sequence analysis of genes is necessary for reliable identification.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 99%

Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens byPCR‐sequencing method inWest ofIran

Dastranj

Farahani

Shahraki

et al. 2017

Clinical Respiratory J

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“…Studies conducted in Japan revealed that the draft genome sequence of Mycolicibacterium peregrinum isolated from a pig affected with lymphadenitis shares close identity with that of Mycolicibacterium peregrinum isolated from the soil of the pig's environment (Komatsu et al 2019). Moreover, the fact that Mycolicibacterium peregrinum was isolated from other animals (e.g., lion; Panthera leo), and from drinking water (Gcebe and Hlokwe 2017;Mohajeri et al 2017) indicates that Mycolicibacterium peregrinum can inhabit several environments. In our study, the rpoB gene of the Mycolicibacterium peregrinum isolated from wildlife showed 100% similarity to that of Mycolicibacterium peregrinum isolated from drinking water (Mohajeri et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Odoi

Ohya

Moribe

et al. 2020

Journal of Wildlife Diseases

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Nontuberculous mycobacteria (NTM) are opportunistic pathogens of humans and animals and are transmitted among the environment, wildlife, livestock, and humans. The aim of this study was to investigate the rate of isolation and antimicrobial susceptibility of NTM in wildlife. In total, 178 samples of feces ( n=131) and tissues ( n=47) were collected from 11 wildlife species in Gifu Prefecture and Mie Prefecture, Japan, between June 2016 and October 2018. We isolated NTM from 15.3% (20/131) of fecal samples using Ogawa medium, and isolates were identified by sequencing the rpoB and hsp65 genes. The rpoB sequences were compared with those from other strains of human and environmental origin. The NTM isolates were obtained from sika deer ( Cervus nippon), wild boar ( Sus scrofa), Japanese monkey ( Macaca fuscata), raccoon dog ( Nyctereutes procyonoides), masked palm civet ( Paguma larvata), and Japanese weasel ( Mustela itatsi) and were classified as rapidly growing mycobacteria (RGM) and slowly growing mycobacteria (SGM). The 12 RGM identified were Mycolicibacterium peregrinum ( n=5), Mycolicibacterium fortuitum ( n=3), Mycolicibacterium septicum ( n=3), and Mycolicibacterium thermoresistibile ( n=1), and the eight SGM were Mycobacterium paraense ( n=4), Mycolicibacter arupensis ( n=2), Mycolicibacter virginiensis ( n=1), and Mycobacterium nebraskense ( n=1). The NTM from wildlife showed ≥99% similarity with strains from different sources including humans. The RGM were susceptible to the antimicrobial agents tested except for M. fortuitum, which was resistant to azithromycin and clarithromycin. The SGM showed multiple drug resistance qualities but were susceptible to amikacin, clarithromycin, and rifabutin. These results indicate that wildlife may be reservoir hosts of NTM in Japan. The presence of antimicrobial-resistant NTM in wildlife suggests that the trends of NTM antimicrobial susceptibility in wildlife should be monitored.

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“…M. mucogenicum was also detected in a similar study by Nasr Esfahani et al [21] in central Iran, where the dominant isolates were M. fortuitum, M. chelonae-like organism, and M. mucogenicum. A similar study by Mohajeri et al [22] evaluating the frequency of NTM in drinking water supplies revealed NTM in 32% of 110 samples, with M. gastri as the most common species.…”

Section: Discussionmentioning

confidence: 52%

Isolation and identification of Nontuberculous mycobacteria from environmental water samples in the northeast of Iran

Akbari¹,

Zare²,

Ghazvini³

2019

mjima

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Giriş: Çevresel insan enfeksiyonu kaynağı olarak son zamanlarda giderek artan düzeyde dikkat çeken ve toplamda 150'den fazla türü olan nontüberküloz mikobakterilere (NTM) verilen önem artmaktadır. Bu çalışmanın amacı, İran'ın kuzeydoğusunda çevresel NTM türlerini izole etmek ve tanımlamaktı. Gereç ve Yöntem: Razavi Horasan eyaletinin 73 şehrindeki içme suyu dağıtım ağları ve su kaynaklarından toplanan 344 su örneği kültür yoluyla incelendi. Nontüberküloz mikobakteriler tanımlaması ayrıca hsp65 geninin polimeraz zincir reaksiyonu amplifikasyonu, sekanslama ve veri analizi yoluyla da yapıldı. İstatistiksel analiz, SPSS 16.0 kullanılarak Pearson korelasyon katsayısı testi ve iki örnek t-testi ile yapıldı. istatistiksel anlamlılık seviyesi 0,05 olarak belirlendi. Bulgular: Nontüberküloz mikobakteriler, kültürde su örneklerinin %10,46'sında (36/244) tespit edilmiştir. En yaygın NTM türü Mycobacterium gordonae (13/36) olarak belirlenmiştir. Diğer saptanan türler M. mucogenicum, M. senegalense, M. gadium, M. genavense, M. simiae, M. frederiksbergense, M. flouranthenivorans, M. neoaurum, ve M. pallens'di. Ayrıca, suda NTM varlığı ile nitrat seviyesi, türbidite, pH ve su kaynağının Introduction: The importance of nontuberculous mycobacteria (NTM), a group including over 150 species, has recently received increased attention as an environmental source of human infection. This study aimed to isolate and identify NTM species in the environment in the northeast of Iran. Materials and Methods: A total of 344 water samples were obtained from water resources and drinking water distribution networks in 73 cities in the Khorasan Razavi province and were examined using culture. Nontuberculous mycobacteria identification was also accomplished by polymerase chain reaction amplification of the hsp65 gene, sequencing and data analysis. Statistical analysis was performed by Pearson's correlation coefficient test and two-sample T-test using SPSS 16.0. Statistical significance was determined at a significance level of 0.05. Results: Nontuberculous mycobacteria were identified in 10.46% of the water samples (36/344) by culture. The most frequent NTM species was Mycobacterium gordonae (13/36). Other Mycobacterium species detected included M . mucogenicum, M. senegalense, M. gadium, M. genavense, M. simiae, M. frederiksbergense, M. flouranthenivorans, M. neoaurum, and M. pallens. Statistically significant correlations were observed between the presence of NTM in water and nitrate level, turbidity, pH, and age of water resource (p<0.001). The relation between NTM presence and depth of the water resource was not statistically significant (p>0.05). Conclusion: It was concluded that NTM water contamination was not very high in this area, but further studies are needed to investigate the sources of contamination and origins of these species.

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Verification of Frequency in Species of Nontuberculous Mycobacteria in Kermanshah Drinking Water Supplies Using the PCR-Sequencing Method

Cited by 16 publications

References 19 publications

Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens byPCR‐sequencing method inWest ofIran

Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens byPCR‐sequencing method inWest ofIran

Isolation and Antimicrobial Susceptibilities of Nontuberculous Mycobacteria From Wildlife in Japan

Isolation and identification of Nontuberculous mycobacteria from environmental water samples in the northeast of Iran

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