Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens by<scp>PCR</scp>‐sequencing method in<scp>W</scp>est of<scp>I</scp>ran

Dastranj, Mahsa; Farahani, Abbas; Shahraki, Abdolrazagh Hashemi; Atashi, Sara Naybandi; Mohajeri, Parviz

doi:10.1111/crj.12617

Cited by 12 publications

(14 citation statements)

References 16 publications

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“…In the present study, we demonstrated that M. fortuitum , M. abscessus , M. simiae , and M. kansasii are the most common RGM and SGM species in samples of patients from different provinces investigated in this study; this finding is in line with other reports from Iran 17,35,36. Moreover, M. fortuitum was reported as the most common NTM, isolated from environmental and clinical sources in the distinctive part of Iran 37,38…”

Section: Discussionsupporting

confidence: 92%

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against Mycobacterium fortuitum and Mycobacterium abscessus

Khosravi¹,

Mirsaeidi²,

Farahani³

et al. 2018

IDR

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BackgroundThe prevalence of pulmonary disease caused by nontuberculous mycobacteria (NTM) is reportedly on the rise in the world. Some of the species are resistant to various antibiotics; hence, limited treatment options are available. The aims of this study were to investigate the prevalence of NTM and to determine the effect of d-cycloserine against Mycobacterium fortuitum and Mycobacterium abscessus isolated from clinical specimens to find out the synergistic effect of d-cycloserine and clarithromycin.MethodsA total of 95 nonduplicate pulmonary isolates of NTM were collected from three major Regional Tuberculosis (TB) Centers. NTM isolates were identified by conventional tests and PCR sequence analysis of the rpoB gene. PCR sequencing of erm-41 was performed for detecting the inducible resistance to macrolides. In vitro susceptibilities and activities of d-cycloserine-clarithromycin combinations were accessed using the broth microdilution method.ResultsAmong 714-positive acid-fast bacilli from TB-suspected cases, 95 isolates were identified as NTM (13.3%). The prevalence of identified isolates was as follows: M. fortuitum 46 (48.4%), Mycobacterium simiae 16 (16.8%), Mycobacterium kansasii 15 (15.7%), M. abscessus 7 (7.3%), Mycobacterium thermoresistibile 4 (4.2%), Mycobacterium elephantis 3 (3.2%), Mycobacterium porcinum 2 (2.1%), and Mycobacterium chimaera 2 (2.1%). In addition, rpoB sequence analysis could identify all NTM isolates. The effect of d-cycloserine was better than that of clarithromycin. The synergistic effect of d-cycloserine with clarithromycin was observed for six (100%) and five (71.5%) strains of M. fortuitum and M. abscessus, respectively.ConclusionIn the present study, we demonstrated a wide range of NTM in processed samples from different provinces of Iran. Our observations indicated that d-cycloserine was very active against M. abscessus and M. fortuitum; hence, d-cycloserine, either alone or in combination with clarithromycin, may be promising for the treatment of M. abscessus- and M. fortuitum-associated diseases.

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Section: Discussionsupporting

confidence: 92%

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against Mycobacterium fortuitum and Mycobacterium abscessus

Khosravi¹,

Mirsaeidi²,

Farahani³

et al. 2018

IDR

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“…A study in Iran showed that among 714 positive acid-fast bacilli from TB-suspected cases, 95 isolates were identified as NTM (13.3%) [38]. Furthermore, many NTMPD patients were infected by a wide range of atypical mycobacteria [39]. erefore, it is of great importance to differentiate NTM from Mycobacterium tuberculosis and to make reliable identification in clinical practice to reduce misdiagnosis and improve treatment efficiency.…”

Section: Discussionmentioning

confidence: 99%

Clinical Characteristics and Antimicrobial Susceptibility of Mycobacterium intracellulare and Mycobacterium abscessus Pulmonary Diseases: A Retrospective Study

Wang

Lin

Cheng

et al. 2022

Canadian Journal of Infectious Diseases and Medical Microbiolog

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The incidence of nontuberculous mycobacteria (NTM) diseases is increasing every year. The present study was performed to investigate the clinical characteristics, CT findings, and drug susceptibility test (DST) results of patients diagnosed with M. intracellulare or M. abscessus nontuberculous mycobacterial pulmonary disease (NTMPD). This retrospective study included patients diagnosed with NTMPD due to M. intracellulare or M. abscessus for the first time at Anhui Chest Hospital between 01/2019 and 12/2021. The patients were grouped as M. intracellulare-NTMPD group or M. abscessus-NTMPD group. Clinical features, imaging data and DST data, were collected. Patients with M. intracellulare infection had a higher rate of acid-fast smears (66.1% vs. 45.2%, P = 0.032 ) and a higher rate of cavitation based on pulmonary imaging (49.6% vs. 19.4%, P = 0.002 ) than patients with M. abscessus infection, but both groups had negative TB-RNA and GeneXpert results, with no other characteristics significant differences. The results of DST showed that M. intracellulare had high susceptibility rate to moxifloxacin (95.9%), amikacin (90.1%), clarithromycin (91.7%), and rifabutin (90.1%). M. abscessus had the highest susceptibility rate to amikacin (71.0%) and clarithromycin (71.0%). The clinical features of M. intracellulare pneumopathy and M. abscessus pneumopathy are highly similar. It may be easily misdiagnosed, and therefore, early strain identification is necessary. M. intracellulare has a high susceptibility rate to moxifloxacin, amikacin, clarithromycin, and rifabutin, while M. abscessus has the highest susceptibility rate to amikacin and clarithromycin. This study provides an important clinical basis for improving the management of NTMPD.

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“…Recently, the overall prevalence of NTM diseases has increased globally and is becoming an emerging health issue. The incidence of pulmonary disease caused by NTM is on the rise, 2 , 26 and that of HIV co-infection has also seen a sudden increase. 4 , 5 However, it is difficult to differentiate NTM-caused diseases from MTBC-caused diseases.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the MeltPro Myco Assay for the Identification of Non-Tuberculous Mycobacteria

Lin¹,

Zhao²,

Wei³

et al. 2022

IDR

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Purpose The purpose of this study was to evaluate a new commercial kit for Mycobacterium species identification and compare its results with that of the commonly used GenoType Mycobacterium CM assay. In addition, we were committed to identifying the main frequent species of nontuberculous mycobacteria (NTM) in Fujian. Methods A total of 261 clinical strains, collected at the Center for Disease Control and Prevention of Fujian Province, China, were preliminarily identified as NTM based on p-nitrobenzoic acid (PNB) growth test. The genomic DNA of all 261 strains was extracted and subjected to species identification using MeltPro Myco assay and GenoType Mycobacterium CM assay. The results of the latter were used as a control to calculate the positive agreement, negative agreement, agreement and the total agreement of the former. For samples with inconsistent detection results, sequencing was performed for verification. Results Compared to GenoType Mycobacterium CM assay, the total agreement of MeltPro Myco assay was 96.55% (252/261 strains). Both the positive and negative agreement of MeltPro Myco assay in identifying M. intracellulare, M. abscessus, M. kansasii, M. fortuitum, M. avium, M. gordonae, M. scrofulaceum , and mixed infections were higher than 99.00%, but the positive agreement of M. chelonae was relatively low at only 33.33%. In Fujian, the predominant strain of NTM was M. intracellulare (64.36%, 130/202 strains), followed by M. abscessus (19.31%, 39/202 strains), M. kansasii (4.46%, 9/202 strains), and M. fortuitum (3.96%, 8/202 strains). Conclusion The reliability of MeltPro Myco assay for identifying mycobacterium species was strongly demonstrated in this study, which greatly supports its usage for the clinical identification of mycobacteria. The present study also showed that the distribution of mycobacteria in Fujian, China, was significantly different from that in other regions and provided important data for future epidemiological study of NTM.

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Molecular identification and distribution of non‐tuberculous mycobacteria isolated from clinical specimens byPCR‐sequencing method inWest ofIran

Cited by 12 publications

References 16 publications

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against <em>Mycobacterium fortuitum </em>and <em>Mycobacterium abscessus</em>

Prevalence of nontuberculous mycobacteria and high efficacy of D-cycloserine and its synergistic effect with clarithromycin against <em>Mycobacterium fortuitum </em>and <em>Mycobacterium abscessus</em>

Clinical Characteristics and Antimicrobial Susceptibility of Mycobacterium intracellulare and Mycobacterium abscessus Pulmonary Diseases: A Retrospective Study

Evaluation of the MeltPro Myco Assay for the Identification of Non-Tuberculous Mycobacteria

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