Vergleichende Untersuchung zum spezifischen Gewicht des peritumoralen, menschlichen Hirngewebes

Schröder, R.

doi:10.1007/bf01405652

Cited by 3 publications

(1 citation statement)

References 33 publications

(13 reference statements)

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“…This results in a volume of 0.002 cm 3 . Brain tissue density has been described to be about 1.04 g/cm 3 35. A maximum of 8 thin sections were used to obtain sufficient amounts of neuromelanin granules, hence roughly up to 16.6 mg tissue was used compared to at least 150 mg up to 1 g brain tissue used a priori114.…”

Section: Discussionmentioning

confidence: 99%

Proteomic characterization of neuromelanin granules isolated from human substantia nigra by laser-microdissection

Plum

Steinbach

Attems

et al. 2016

Sci Rep

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Neuromelanin is a complex polymer pigment found primarily in the dopaminergic neurons of human substantia nigra. Neuromelanin pigment is stored in granules including a protein matrix and lipid droplets. Neuromelanin granules are yet only partially characterised regarding their structure and function. To clarify the exact function of neuromelanin granules in humans, their enrichment and in-depth characterization from human substantia nigra is necessary. Previously published global proteome studies of neuromelanin granules in human substantia nigra required high tissue amounts. Due to the limited availability of human brain tissue we established a new method based on laser microdissection combined with mass spectrometry for the isolation and analysis of neuromelanin granules. With this method it is possible for the first time to isolate a sufficient amount of neuromelanin granules for global proteomics analysis from ten 10 μm tissue sections. In total 1,000 proteins were identified associated with neuromelanin granules. More than 68% of those proteins were also identified in previously performed studies. Our results confirm and further extend previously described findings, supporting the connection of neuromelanin granules to iron homeostasis and lysosomes or endosomes. Hence, this method is suitable for the donor specific enrichment and proteomic analysis of neuromelanin granules.

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Section: Discussionmentioning

confidence: 99%