Neuropathologie

Peiffer, J.

doi:10.1007/978-3-642-50221-7_1

Cited by 4 publications

(2 citation statements)

References 768 publications

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“…Gliomas, the most common type of brain tumors, are characterized by their highly invasive behavior [9]. The progression of low-grade to high-grade glioma is often characterized by an increase in neovascularization, focal necrosis, and cellular proliferation.…”

Section: Introductionmentioning

confidence: 99%

Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor

Chintala

Mohanam

et al. 1997

Mol. Carcinog.

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The interaction of urokinase-type plasminogen activator (uPA) with its cell-surface receptor (uPAR) is implicated in diverse biological processes such as cell migration, tissue remodeling, and tumor cell invasion. Recent studies indicated that uPAR can act as an extracellular matrix receptor during cell adhesion. Recently, we showed that transfection of the human glioma cell line SNB19 with antisense uPAR resulted in downregulation of uPAR at both the mRNA and protein levels. In this study, we used SNB19 to determine how the presence or absence of uPAR promotes cell spreading and associated changes in cell morphology. Microscopic analysis of cell spreading revealed that antisense uPAR-transfected cells were larger, remained round, and did not spread efficiently over extracellular matrix substrate type IV collagen and fibronectin, unlike parental SNB19 cells, which were smaller and spindle shaped. Biochemical studies showed that antisense uPAR-transfected cells, in addition to not spreading, exhibited increased expression of alpha 3 beta 1 integrin but not alpha 5 beta 1 integrin. However, we could not find a change in the expression of extracellular matrix components or altered growth rate in these cells. Furthermore, despite the increased alpha 3 beta 1 integrin expression, antisense uPAR-transfected cells failed to form an organized actin cytoskeleton when plated on type IV collagen or fibronectin, unlike parental SNB19 cells, which displayed an organized cytoskeleton. These findings show that the absence of uPAR in human glioma cells leads to morphological changes associated with decreased spreading and a disorganized cytoskeleton resulting in altered cell morphology, suggesting that coordinated expression of uPAR and integrin may be involved in spreading of antisense uPAR-transfected glioma cells.

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Section: Introductionmentioning

confidence: 99%

Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor

Chintala

Mohanam

et al. 1997

Mol. Carcinog.

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“…Gliomas, the most common type of human brain tumors, are characterized by their ability to be highly invasive [1]. Gliomas display a wide variety of distinct histological appearances.…”

Section: Introductionmentioning

confidence: 99%

Role of extracellular matrix proteins in regulation of human glioma cell invasion in vitro

Chintala

Gokaslan

et al. 1996

Clin Exp Metast

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Primary brain tumors lack the metastatic behavior that is in part believed to be promoted by the extracellular matrix (ECM) components of the basement membrane. This study was intended to examine the influence of the ECM components present in the basement membrane that may act as natural barriers to tumor cell invasion. We examined the effect of type I and type IV collagens, fibronectin, laminin, and hyaluronic acid on the migration and invasion of four established glioblastoma cell lines, SNB19, U251, UWR1, and UWR2. Lower concentrations of all the ECM components induced the migration and invasion of all the cell lines. However, in the case of SNB19, laminin inhibited both migration and invasion in a concentration-dependent manner. We have also examined the influence of individual ECM components on the migration of cells from a spheroid to a monolayer on ECM component-coated coverslips. Consistent with the invasion studies using the modified Boyden chamber assays, lower concentrations of ECM components induced the migration of cells from spheroids to monolayer. Again, laminin inhibited the migration of cells from SNB19 spheroids. These results indicate that ECM components induce the invasion of glioma cells, apart from components like laminin, which may act as natural inhibitors.

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Stereotactic linear accelerater‐based radiosurgery for the treatment of patients with glomus jugulare tumors

Maarouf

Voges

Landwehr

et al. 2003

Cancer

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Activated layered double hydroxides (LDHs) with high crystallinity, obtained by calcination/rehydration of LDH precursors synthesized by urea decomposition, have higher catalytic activity in acetone self‐condensation and Knoevenagel reactions than less crystalline materials obtained from LDH precursors synthesized by titration co‐precipitation. The activated LDHs possess both basic and acidic sites. High resolution transmission electron microscopy (HRTEM) confirms that the highly crystalline activated LDHs retain the lattice structure of the LDH precursors with lattice parameters a = b = 0.31 ± 0.01 nm and α = 60 ± 2°. An acid‐base catalytic mechanism has been proposed to interpret the catalytic behavior based on the fact that acid‐base hydroxyl group pairs on the activated LDH surface have a separation of 0.31 nm. It is proposed that the active sites are mainly located on the ordered array of hydroxyl sites on the basal surfaces rather than on the edges, as has been previously suggested. © 2007 American Institute of Chemical Engineers AIChE J, 2007

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Neuropathologie

Cited by 4 publications

References 768 publications

Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor

Altered in vitro spreading and cytoskeletal organization in human glioma cells by downregulation of urokinase receptor

Role of extracellular matrix proteins in regulation of human glioma cell invasion in vitro

Stereotactic linear accelerater‐based radiosurgery for the treatment of patients with glomus jugulare tumors

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