Veratridine-sensitive Na + channels regulate human sperm fertilization capacity

Candenas, M. Luz; Pinto, Francisco M.; Cejudo-Román, Antonio; González-Ravina, Cristina; Sánchez, Manuel Fernández; Pérez-Hernández, Natalia; Irazusta, Jon; Subirán, Nerea

doi:10.1016/j.lfs.2018.01.004

Cited by 11 publications

(14 citation statements)

References 45 publications

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“…Thus, in this study, the ADP and AMP could be investigated, with ADP decreasing and AMP increasing in the sperm treated with MZ. Moreover, the sperm exposed to MZ reduced EC and ADP/AMP ratio because MZ was found to decrease the potassium in ion channels’ activity [ 30 ], resulting in decreased sperm motility [ 31 ]. Nevertheless, the ADP level, EC, and ADP/AMP ratio were increased in the sperm induced by MZ, co-administrated with WNAE, and co-administrated with Vit E, but the AMP level was decreased because Vit E and flavonoids in WNAE extract are strong antioxidants [ 20 , 22 , 23 , 24 , 32 ] with the potential for free radical scavenging, resulting in increased energy preservation, sperm motility, sperm viability, acrosome integrity, and normal sperm morphology.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Laoung-on

Jaikang

Saenphet

et al. 2022

Plants

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The white Nelumbo nucifera petals aqueous extraction (NAE) was prominent in phytochemical content, antioxidant activity, and enhanced rat sperm viability induced by FeSO4, a heavy metal. Mancozeb (MZ) contains heavy metals and is widely used for fungal control in agriculture and industry. It induces oxidative stress and causes of spermatogenesis and reproductive organs’ abnormalities in both humans and animals. The aims of the present study were to investigate the effects of white Nelumbo nucifera petals aqueous extraction (WNAE) on sperm quality in cattle sperm induced by MZ. Moreover, this study investigated phytochemical compounds by liquid chromatography-mass spectrometry. A protein profile related to sperm quality with SDS-page and sperm energy preservation for each treatment was determined. The results found nine phytochemical compounds, in which quercetin-3-O-arabinoglycoside was a major flavonoid that was found in the WNAE. MZ induced free radicals in cells, leading to LPO and protein oxidation, while decreasing sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The cattle sperm found four proteins related to sperm quality including MWs of 17, 31, 34, and 55 kDa. The WNAE effectively increased energy preservation, sperm motility, sperm viability, acrosome integrity, and normal sperm morphology. The WNAE enhanced sperm qualities by reducing oxidative stress. It might be suggested that WNAE has benefits for sperm preservation which may be used to guard against toxicity in animals or humans exposed to MZ contaminants.

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Section: Discussionmentioning

confidence: 99%

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Laoung-on

Jaikang

Saenphet

et al. 2022

Plants

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“…It is very likely that this specific distribution reflexes signaling and metabolic pathways in spermatozoa. A role of VGNCs in sperm cells is the maintenance of progressive motility rather than hyperactivation, although they are also engaged in capacitation through the tyrosine phosphorylation of proteins localized in the post-equatorial region, midpiece, and flagellum [163,164]. In human spermatozoa, pharmacological stimulation of Na v 1.8 has increased progressive motility but has not induced hyperactivation or an acrosome reaction [161].…”

Section: Voltage-gated Na + Channels (Vgncs Nav)mentioning

confidence: 99%

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Nowicka-Bauer

Szymczak-Cendlak

2021

IJMS

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Sperm motility is linked to the activation of signaling pathways that trigger movement. These pathways are mainly dependent on Ca2+, which acts as a secondary messenger. The maintenance of adequate Ca2+ concentrations is possible thanks to proper concentrations of other ions, such as K+ and Na+, among others, that modulate plasma membrane potential and the intracellular pH. Like in every cell, ion homeostasis in spermatozoa is ensured by a vast spectrum of ion channels supported by the work of ion pumps and transporters. To achieve success in fertilization, sperm ion channels have to be sensitive to various external and internal factors. This sensitivity is provided by specific channel structures. In addition, novel sperm-specific channels or isoforms have been found with compositions that increase the chance of fertilization. Notably, the most significant sperm ion channel is the cation channel of sperm (CatSper), which is a sperm-specific Ca2+ channel required for the hyperactivation of sperm motility. The role of other ion channels in the spermatozoa, such as voltage-gated Ca2+ channels (VGCCs), Ca2+-activated Cl-channels (CaCCs), SLO K+ channels or voltage-gated H+ channels (VGHCs), is to ensure the activation and modulation of CatSper. As the activation of sperm motility differs among metazoa, different ion channels may participate; however, knowledge regarding these channels is still scarce. In the present review, the roles and structures of the most important known ion channels are described in regard to regulation of sperm motility in animals.

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“…They undergo capacitation, acquire hyperactive motility and change their membrane composition through the stripping and/or modification of many glycoproteins that coat the sperm surface and the bidirectional movement of phospholipids across the lipid bilayer [ 7 , 77 ]. This change is stimulated by bicarbonate that rapidly activates sperm-specific adenylate cyclase (sACY), leading to the generation of cAMP and an increase in phosphorylation of proteins in serine, threonine and tyrosine residues [ 78 , 79 , 80 , 81 ]. Prostasomes help to increase intracellular cAMP levels [ 82 ].…”

Section: Exosomes In the Male Reproductive Tract: Their Potential mentioning

confidence: 99%

“…Prostasomes help to increase intracellular cAMP levels [ 82 ]. During sperm cell capacitation, there is an activation of epithelial and voltage Na+ channels and of K+ channels, with subsequent hyperpolarization of the plasma membrane, increase in pH and opening of Ca 2+ channels [ 79 , 80 , 81 , 83 ].…”

Section: Exosomes In the Male Reproductive Tract: Their Potential mentioning

confidence: 99%

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

Candenas

Chianese

2020

IJMS

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Infertility has become a global health issue, with approximately 50% of infertility cases generated by disorders in male reproduction. Spermatozoa are conveyed towards female genital tracts in a safe surrounding provided by the seminal plasma. Interestingly, this dynamically changing medium is a rich source of proteins, essential not only for sperm transport, but also for its protection and maturation. Most of the seminal proteins are acquired by spermatozoa in transit through exosomes (epididymosomes and prostasomes). The high number of seminal proteins, the increasing knowledge of their origins and biological functions and their differential expression in the case of azoospermia, asthenozoospermia, oligozoospermia and teratozoospermia or other conditions of male infertility have allowed the identification of a wide variety of biomarker candidates and their involvement in biological pathways, thus to strongly suggest that the proteomic landscape of seminal plasma may be a potential indicator of sperm dysfunction. This review summarizes the current knowledge in seminal plasma proteomics and its potentiality as a diagnostic tool in different degrees of male infertility.

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Veratridine-sensitive Na + channels regulate human sperm fertilization capacity

Abstract: Our results suggest that veratridine-sensitive Na channels are involved on human sperm fertility acquisition regulating motility, capacitation and the progesterone-induced acrosome reaction in human sperm.

Cited by 11 publications

References 45 publications

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Effect of Nelumbo nucifera Petals Extract on Antioxidant Activity and Sperm Quality in Charolais Cattle Sperm Induced by Mancozeb

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Exosome Composition and Seminal Plasma Proteome: A Promising Source of Biomarkers of Male Infertility

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