Venturia nashicola, the scab fungus of Japanese and Chinese pears: a species distinct from V. pirina

Ishii, Hideo; Yanase, H.

doi:10.1017/s0953756299001720

Cited by 56 publications

(56 citation statements)

References 4 publications

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“…"Flemish Beauty" 16,41,44 , but not in Japanese or Chinese pears 19 . V. nashicola and V. pirina can be distinguished through morphological characterization of the size of conidia and ascospores 19,44 and the internal transcribed spacer (ITS) region of the rDNA of these 2 species 2 . Amplification of a 391-bp DNA fragment within the ITS region by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the PCR products can enable V. nashicola to be dis-tinguished from other related fungi, including V. pirina 26 ; however, these techniques do not identify each race of the V. nashicola, and pathogenicity tests are required 17 to distinguish between the races, which are time consuming and laborious.…”

Section: Introductionmentioning

confidence: 97%

“…Venturia pirina causes a scab disease in European pears such as cv. "Flemish Beauty" 16,41,44 , but not in Japanese or Chinese pears 19 . V. nashicola and V. pirina can be distinguished through morphological characterization of the size of conidia and ascospores 19,44 and the internal transcribed spacer (ITS) region of the rDNA of these 2 species 2 .…”

Section: Introductionmentioning

confidence: 97%

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Cloning and Sequence Analysis of Endopolygalacturonase Genes in Venturia nashicola and Venturia pirina

Katoh

Yamada

Akimitsu

et al. 2011

JARQ

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Genes encoding endopolygalacturonase (endoPG) were isolated from pathogens of the Asian pear scab, Venturia nashicola races 1, 2, 3, and 4, and European pear scab, V. pirina. The Vnpgr1 gene of the V. nashicola race 1 consists of a 1,116-bp open reading frame, encoding a protein of 372 amino acids with an estimated molecular mass of 37.5 kDa and an isoelectric point (pI) of 6.56. The sequences of Vnpg genes from different races and Vppg gene from V. pirina showed high identities (95-100%). The deduced amino acid sequence of the V. nashicola race 1 endoPG showed 63-68% identity to the endoPG sequences of Penicillium olsonii, Colletotrichum lindemuthianum, Cryphonectria parasitica, Fusarium oxysporum, Sclerotinia sclerotiorum, and Alternaria citri. The deduced amino acid sequence of the race 1 endoPG was identical to the Nterminal amino acid sequence of the previously purified endoPG enzyme from the mycelia of this race. The results of a southern blot analysis indicated that V. nashicola race 1 (isolate JS-115) had a single copy of the Vnpgr1. Restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR) products of the endoPG gene digested with HincII, BspEI, and BsrGI was performed; thereafter, agarose gel electrophoresis yielded race-specific RFLP patterns.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Cloning and Sequence Analysis of Endopolygalacturonase Genes in Venturia nashicola and Venturia pirina

Katoh

Yamada

Akimitsu

et al. 2011

JARQ

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“…on European pear {Pyrus communis L.) (Villalta et al 2004) and V. nashicola Tanaka & Yamamoto on Asian pear (Ishii & Yanase 2000), is a serious disease that can cause heavy crop losses if left untreated (Chevalier et al 2002;Villalta et al 2004;Abe et al 2008). It is most severe in regions where regular rainfall or heavy dews occur in the spring when young leaves are expanding and are at their most susceptible (Li & Zhao 2001;Villalta et al 2004;Spotts et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Resistance to scab caused byVenturis pirinain interspecific pear (Pyrusspp.) hybrids

Brewer¹,

Alspach

Morgan

et al. 2009

New Zealand Journal of Crop and Horticultural Science

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The genetics of resistance to Venturia pirina was investigated from 2002 to 2004 on 11 families derived from interspecific crosses between European and Asian pear species. Nearly complete leaf resistance to V. pirina was identified in all progeny of families with at least 50% Asian pear (Pyrus pyrifolia and/or Pyrus × bretschneideri) ancestry, but up to 26% of these trees exhibited fruit scab incidence in any one of three years. However, between 67% and 80% of the scab leaf resistant individuals had fruit russet spotting owing to scab infection in one or more years over the duration of the trial and between 14% and 36% of the leaves were affected by russet spot. The level of affected individuals did not appear to decrease with increasing proportion of Asian parentage for leaves or fruit. In contrast, reference families derived from European pears and many of the families with 12-25% Asian parentage were almost 100% susceptible to scab on both leaves and fruit. In European families, only 2% of these individuals had leaves affected by russet spotting, whereas an average of 23% of fruit were affected. The genetics of non-host resistance is discussed.

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“…culta Nakai) and Chinese pear (P. ussuriensis Maxim) (Ishii and Yanase 2000;Takana and Yamamoto 1964). Almost all commercial cultivars of Japanese pear are susceptible, resulting in a high dependence on fungicides to control this disease.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression analysis of genes related to phosphatidic acid synthesis in Japanese pear leaves inoculated with Venturia nashicola

Zheng

Ishii

2009

J Gen Plant Pathol

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Phosphatidic acid, which can be generated by the action of phospholipase D (PLD) and by the combined action of phospholipase C (PLC) and diacylglycerol kinase (DGK), is a plant defense signal. To identify the role of the PLD, PLC, and DGK genes encoding these enzymes in pear resistance to the scab fungus Venturia nashicola, we report the cloning of these genes and analysis of their expression in inoculated pear leaves. The results showed that PLD mRNA increased in the leaves of the susceptible pear cv. Kousui 1 day after inoculation (dai), then decreased to the basal level. In resistant pear cv. Kinchaku, PLD mRNA did not change significantly except for a small increase at 2 and 3 dai. However, the level of PLD mRNA in Kinchaku was higher than in Kousui 2 dai. The level of PLC mRNA significantly increased in cvs. Kousui and Kinchaku by 1 dai, peaked at day 2, then decreased to the basal level. Thus, the gene expression pattern did not differ between the two cultivars. PLC gene expression was also induced by environmental stress. The DGK gene seemed to be constitutively expressed and was not induced by inoculation in either the susceptible or resistant cultivars. Together, this data showed that both PLD and PLC are possibly involved in a defense response to V. nashicola in Japanese pear leaves, and the PLD gene seems to play a more important role than does the PLC gene.

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Venturia nashicola, the scab fungus of Japanese and Chinese pears: a species distinct from V. pirina

Cited by 56 publications

References 4 publications

Cloning and Sequence Analysis of Endopolygalacturonase Genes in Venturia nashicola and Venturia pirina

Cloning and Sequence Analysis of Endopolygalacturonase Genes in Venturia nashicola and Venturia pirina

Resistance to scab caused byVenturis pirinain interspecific pear (Pyrusspp.) hybrids

Molecular cloning and expression analysis of genes related to phosphatidic acid synthesis in Japanese pear leaves inoculated with Venturia nashicola

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