Ventral Expression of GATA-1 and GATA-2 in the Xenopus Embryo Defines Induction of Hematopoietic Mesoderm

Kelley, Clair M.; Yee, Karen O.; Harland, Richard M.; Zon, Leonard I.

doi:10.1006/dbio.1994.1246

Cited by 121 publications

(76 citation statements)

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“…One possible candidate factor seemed to be the zinc finger protein GATA-2 (20), which activates Xvent-1, and when overexpressed as a dominant-interfering construct, had been shown to suppress specifically expression of the Xvent-1 but not of the Xvent-2 gene (21). The GATA-2 gene is induced by BMP-4 (22), and GATA-2 expression in ventral mesoderm starts at the early gastrula stage (23), i.e. at the right time and at the right place to suggest this factor as an additional player in the in vivo regulation of Xvent-1 transcription.…”

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confidence: 99%

Cooperative Interaction of Xvent-2 and GATA-2 in the Activation of the Ventral Homeobox Gene Xvent-1B

Friedle

Knöchel

2002

Journal of Biological Chemistry

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The Xvent family of homeobox transcription factors is essential for the establishment of the dorsal-ventral body axis during Xenopus embryogenesis. In contrast to Xvent-2B and other members of the Xvent-2 subfamily, Xvent-1B is not a direct response gene of bone morphogenetic protein-4 signaling. Xvent-1B is activated by Xvent-2, but CHX experiments revealed the requirement of additional factors. In this study, we report on the cooperative effect of Xvent-2 and the zinc finger transcription factor GATA-2 on the promoter of the Xvent-1B gene. We show that GATA-2 is a direct target gene of bone morphogenetic protein-4 and that GATA-2 interacts with Xvent-2 to activate transcription of Xvent-1B. Both transcription factors bind to distinct elements within the Xvent-1B promoter, and GATA-2 physically interacts with the C-terminal domain of Xvent-2. Promoter/reporter studies in Xenopus embryos revealed that full activation of Xvent-1B requires both Xvent-2 and GATA-2. Moreover, the two factors are sufficient to direct transcription of Xvent-1B in the presence of CHX at the ventral side of the embryo. The failure of both factors to activate Xvent-1B on the dorsal side suggests the existence of a dorsal inhibitor. This inhibitor is likely a component of the dorsal Wnt signaling pathway because nuclear translocation of ␤-catenin before midblastula transition results in a suppression of Xvent-1B transcription.

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confidence: 99%

Cooperative Interaction of Xvent-2 and GATA-2 in the Activation of the Ventral Homeobox Gene Xvent-1B

Friedle

Knöchel

2002

Journal of Biological Chemistry

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“…GATA-2 is expressed in a wide variety of tissues, which include hematopoietic progenitors, erythroid cells, mast cells, megakaryocytes, endothelial cells, the central nervous system, and the giant cells of the trophoblast (11)(12)(13)(14)(15)(16)(17)(18)(19)(20).…”

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confidence: 99%

“…During erythroid maturation, GATA-2 mRNA is down-regulated (11,21), whereas ectopic overexpression in chicken erythroid progenitors of GATA-2, but not GATA-1 and GATA-3, promotes their proliferation at the expense of their differentiation (22). In Xenopus and zebrafish, GATA-2 is expressed within the presumptive blood island of the embryo (16,18,23). Taken together, these data indicate that GATA-2 has a critical role in early hematopoietic cells, possibly influencing the maintenance or the proliferation of the progenitors.…”

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confidence: 99%

Alternate Promoters and Developmental Modulation of Expression of the Chicken GATA-2 Gene in Hematopoietic Progenitor Cells

Nony

Hannon

Gould

et al. 1998

Journal of Biological Chemistry

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We have isolated and characterized the chicken GATA-2 (cGATA-2) gene. We show that, as in the case of some other members of the GATA gene family, the gene is expressed from alternative first exons. One of the resulting mRNAs represents only a minor form of the GATA-2 mRNA in the cells and tissues we analyzed; the other is ubiquitously expressed. We have defined the minimal promoter that controls expression of this most abundant mRNA and that is necessary for full activity in hematopoietic progenitor cells. The activity of this promoter in transient assays is consistent with developmental differences of expression levels in these cells. We identify within the promoter a previously unrecognized extended CCAAT motif essential for its activity. The organization of the cGATA-2 gene, with alternative first exons and a CCAAT box in the proximal promoter, is similar to that recently described for mouse GATA-2, and the proximal promoter also resembles the only promoter so far described in Xenopus. Nonetheless, the roles of the promoters in development and tissue-specific expression are quite different in these organisms, most strikingly in the mouse, which assigns developmental roles to its proximal and distal promoters that are quite different from those in the chicken. We suggest that although the overall organization may remain the same, the role assigned to each promoter varies among organisms. We identify distant upstream regulatory elements in the cGATA-2 gene that modulate expression from the proximal promoter and that may be responsible for this variation.

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“…The erythropoietic transition from the VBI to the DLP is dependent on the THs (Rollins-Smith and Blair, 1990). The expressions of globin and GATA-1 genes, terminal differentiation markers of erythroid cells, are detected exclusively in the VBI of tailbud embryos (Kelley et al, 1994;Bertwistle et al, 1996), suggesting further that the VBI is the sole source of primitive erythroid cells in X. laevis. The different responses in larval and adult RBCs to THs, apoptotic cell death in larval but not in adult RBCs in X. laevis (Figs.…”

Section: Erythropoietic Organs In Anuransmentioning

confidence: 95%

Erythropoiesis and Conversion of RBCs and Hemoglobins from Larval to Adult Type during Amphibian Development

Wakahara

Yamaguchi

2001

Zoological Science

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Ventral Expression of GATA-1 and GATA-2 in the Xenopus Embryo Defines Induction of Hematopoietic Mesoderm

Cited by 121 publications

References 0 publications

Cooperative Interaction of Xvent-2 and GATA-2 in the Activation of the Ventral Homeobox Gene Xvent-1B

Cooperative Interaction of Xvent-2 and GATA-2 in the Activation of the Ventral Homeobox Gene Xvent-1B

Alternate Promoters and Developmental Modulation of Expression of the Chicken GATA-2 Gene in Hematopoietic Progenitor Cells

Erythropoiesis and Conversion of RBCs and Hemoglobins from Larval to Adult Type during Amphibian Development

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