Venomics of Vipera berus berus to explain differences in pathology elicited by Vipera ammodytes ammodytes envenomation: Therapeutic implications

Latinović, Zorica; Leonardi, Adrijana; Šribar, Jernej; Sajevic, Tamara; Žužek, Monika C.; Frangež, Robert; Halassy, Beata; Trampus-Bakija, A; Pungerčar, Jože; Križaj, Igor

doi:10.1016/j.jprot.2016.06.020

Cited by 56 publications

(69 citation statements)

References 78 publications

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“…Although the venom of V. berus has been extensively studied in the past 20 years, the main venom characteristics in different localities and populations remain unknown. Most studies involved V. b. berus from Russian and France (Guillemin et al, 2003;Malenev et al, 2007;Ramazanova et al, 2008;Latinović et al, 2016), while one used V. berus venom from former Czechoslovakia (Mebs and Langelüddeke, 1992). These V. b. berus venoms contained oedema-forming, anti-haemostatic, anticoagulant, fibrinolytic, proteolytic, haemorrhagic and myotoxic components (Saint Girons and Detrait, 1978; Mebs and Langelüddeke, 1992;Calderón et al, 1993;Samel et al, 2003;Latinović et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Individual variability of venom from the European adder (Vipera berus berus) from one locality in Eastern Hungary

Malina¹,

Krecsák²,

Westerström

et al. 2017

Toxicon

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We have revealed intra-population variability among venom samples from several individual European adders (Vipera berus berus) within a defined population in Eastern Hungary. Individual differences in venom pattern were noticed, both gender-specific and age-related, by one-dimensional electrophoresis. Gelatin zymography demonstrated that these individual venoms have different degradation profiles indicating varying protease activity in the specimens from adders of different ages and genders. Some specimens shared a conserved region of substrate degradation, while others had lower or extremely low protease activity. Phospholipase A activity of venoms was similar but not identical. Interspecimen diversity of the venom phospholipase A-spectra (based on the components' molecular masses) was detected by MALDI-TOF MS. The lethal toxicity of venoms (LD) also showed differences among individual snakes. Extracted venom samples had varying neuromuscular paralysing effect on chick biventer cervicis nerve-muscle preparations. The paralysing effect of venom was lost when calcium in the physiological salt solution was replaced by strontium; indicating that the block of twitch responses to nerve stimulation is associated with the activity of a phospholipase-dependent neurotoxin. In contrast to the studied V. b. berus venoms from different geographical regions so far, this is the first V. b. berus population discovered to have predominantly neurotoxic neuromuscular activity. The relevance of varying venom yields is also discussed. This study demonstrates that individual venom variation among V. b. berus living in particular area of Eastern Hungary might contribute to a wider range of clinical manifestations of V. b. berus envenoming than elsewhere in Europe.

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Section: Introductionmentioning

confidence: 99%

Individual variability of venom from the European adder (Vipera berus berus) from one locality in Eastern Hungary

Malina¹,

Krecsák²,

Westerström

et al. 2017

Toxicon

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“…The asterisked data set is a result of this study. The origins of toxin ratios are marked top to bottom numerically: 1 - Göçmen et al 18 , 2 - Petras et al 74 , 3 - Latinović et al 75 , 4 - Al-Shekhadat et al 76 , 5 - Hempel et al 20 . Snake images correspond in the order to the cladogram species.…”

Section: Resultsmentioning

confidence: 99%

“…Further, we reviewed and compared the previously published venomics data of four closely related and geographically proximal Vipera species in relation to V. anatolica ( Figure 5 ). These include the Caucasian V. kaznakovi 74 , the Eurasian V. berus berus 75, 76 , and two subspecies of V. ammodytes occurring in Greece and Turkey, V. a. montandoni and V. a. transcaucasiana . 20 All of these studies used a consistent snake venomics workflow: quantification with RP-HPLC, 1D-PAGE and single band bottom-up.…”

Section: Resultsmentioning

confidence: 99%

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Extended snake venomics by top-down in-source decay: Investigating the newly discovered Anatolian Meadow viper subspecies,Vipera anatolica senliki

Hempel

Damm

Mrinalini

et al. 2019

Preprint

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13Herein we report on the venom proteome of Vipera anatolica senliki, a recently discovered and 14 hitherto unexplored subspecies of the critically endangered Anatolian Meadow viper endemic to the 15 Antalya Province of Turkey. Integrative venomics, including venom gland transcriptomics as well as 16 complementary bottom-up and top-down proteomic analyses, were applied to fully characterize the 17 venom of V. a. senliki. Furthermore, the classical top-down venomics approach was extended to 18 elucidate the venom proteome by an alternative in-source decay (ISD) proteomics workflow using the 19 reducing matrix 1,5-diaminonaphthalene (1,5-DAN). Top-down ISD proteomics allows for disulfide 20 bond mapping as well as effective de novo identification of high molecular weight venom constituents, 21 both of which are difficult to achieve by commonly established top-down approaches. Venom gland 22 transcriptome analysis identified 42 venom transcript annotations from 13 venom toxin families. 23 Relative quantitative snake venomics revealed snake venom metalloproteinases (svMP, 42.9%) as 24 the most abundant protein family, followed by several less dominant toxin families. Online mass 25 profiling and top-down venomics provide a detailed insight into the venom proteome of V. a. senliki 26 and facilitates a comparative analysis of venom variability for the closely related subspecies, V. a. 27 anatolica.28

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“…As such, venom components are of wide interest to many research groups and have been the subject of extensive research. As about 90% of venom components are of peptide origin, all venom analyses use increasingly fast developing proteomic techniques, including electrophoresis [8][9][10][11], immunodetection techniques [12][13][14][15], different types of chromatography [16][17][18][19] and mass spectrometry [20][21][22][23]. Each proteomic method has specific requirements for both the sample components and the protein concentration range.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for Measuring Protein Concentration in Venom Samples

Bocian

Sławek

Jaromin

et al. 2020

Animals

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Simple Summary: Snake venom is mostly composed of proteins and peptides, which are of interest to many researchers due to their potential pharmacological properties. Due to their biochemical character, these components are analyzed using proteomic techniques such as electrophoresis, chromatography and mass spectrometry. A very important stage of such studies is the measurement of protein concentration in the sample, which is most often performed by colorimetric methods. In the presented article, we used five such techniques on venoms of two snake species, namely Agkistrodon contortrix and Naja ashei. In the case of A. contortrix venom, four methods provide similar concentration values, whereas, in the case of N. ashei, the differences between results are very significant. The source of these differences should probably be seen in the differences in amino acid composition of proteins of these two venoms. With this report, we would like to draw attention to the need to select an appropriate method for measuring the concentration of protein in the venom, especially in the case of Elapid species.Abstract: Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.

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Venomics of Vipera berus berus to explain differences in pathology elicited by Vipera ammodytes ammodytes envenomation: Therapeutic implications

Cited by 56 publications

References 78 publications

Individual variability of venom from the European adder (Vipera berus berus) from one locality in Eastern Hungary

Individual variability of venom from the European adder (Vipera berus berus) from one locality in Eastern Hungary

Extended snake venomics by top-down in-source decay: Investigating the newly discovered Anatolian Meadow viper subspecies,Vipera anatolica senliki

Comparison of Methods for Measuring Protein Concentration in Venom Samples

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