VEGFc and VEGFR3 expression in human thyroid pathologies

Шушанов, С. С.; Bronstein, M. E.; Adélaı̈de, José; Jussila, Lotta; Tchipysheva, Tamara A.; Jacquemier, Jocelyne; Stavrovskaya, A. V.; Birnbaum, Daniel; Карамышева, А. Ф.

doi:10.1002/(sici)1097-0215(20000401)86:1<47::aid-ijc7>3.0.co;2-r

Cited by 40 publications

(27 citation statements)

References 20 publications

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“…Since PTC can metastasize via the lymphatic channel to a regional cervical lymph node [22][23][24][25][26], and increased LVD is assumed to be one of the most important parameters associated with the aggressive behavior of a malignant tumor [26], we hypothesized that nodules with more LVD would have an increased risk of LNM and it would be clinically useful if immunohistochemical staining that can predict the risk of LNM and US features that correlates with the risk of LNM. We do not know whether the existing tumor quantile in group 2 (22% vs. 77.4% p<0.001) and similar results were found in a comparison of the 2 quantiles with peritumoral D2-40 staining in the two groups (20.6% vs. 79.4% p=0.000).…”

Section: Clinico-pathological Factorsmentioning

confidence: 80%

Papillary thyroid carcinoma involving cervical neck lymph nodes: correlations with lymphangiogenesis and ultrasound features

et al. 2012

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Section: Clinico-pathological Factorsmentioning

confidence: 80%

Papillary thyroid carcinoma involving cervical neck lymph nodes: correlations with lymphangiogenesis and ultrasound features

et al. 2012

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“…For example, in a study with gastric cancer samples, it could be demonstrated that VEGFR-3 expression is localized on endothelial cells of lymphatic vessels and that VEGF-C secreted from cancer cells may directly induce the proliferation of lymphatic vessels in the stroma of primary gastric cancer; similar mechanisms have been proposed in thyroid tissue from patients with auto-immune disease. 41,42 Several reports have reported elevated levels of VEGFR-3 expression in tumors. 41,43,44 Nevertheless, a lack of correlation between density of a variety of lymphatic markers and lymphatic metastasis in cancer patients has also been reported.…”

Section: Discussionmentioning

confidence: 99%

Immunodetection and quantification of vascular endothelial growth factor receptor‐3 in human malignant tumor tissues

Bando

Brokelmann

Toi

et al. 2004

Intl Journal of Cancer

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Vascular endothelial growth factor recpetor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ ml. The assay was specific for VEGFR-3, with no crossreactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, breast, colon, gastric and lung cancer tissues the protein levels of VEGFR-3 were in the range of 0.6 -16.7 ng/mg protein. Importantly, the level of VEGFR-3 protein detected in the ELISA correlated significantly with the number of VEGFR-3 positive vessels observed in histochemical sections, suggesting that the ELISA assay may be a reliable surrogate of measuring VEGFR-3-positive vessel density. The protein levels of VEGFR-3 in 27 renal cell carcinoma samples had a significant correlation with the levels of VEGF-C (p<0.001), or biological active, free VEGF-A (p<0.0001), but not with VEGFR-1 or total VEGF-A. This assay provides a useful tool for the investigations of the expression levels of VEGFR-3 in physiological and pathological processes, particular in cancer and in lymphangiogenesisrelated disease.

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“…The adjacent tissues were submitted for histopathologic study, which revealed that most of the cells were malignant. Tissues were staged according to the UICC TNM classification, 5th edition (Sobin and Wittekind, 1997). The mode of cancer invasion(MI) was histologically classified as described previously (Yamamoto et al, 1983).In 30 cases, tissue samples of cervical lymph nodes(CLN) were also available for analysis, thereinto, 20 cases were metastatic lymph nodes(LNM: CLN with cancer cells infiltrate).…”

Section: Patients and Tissue Samplesmentioning

confidence: 91%

“…Primer 1 for VEGFR-3 mRNA was used as reported by Shushanov et al (2000), whereas other primers were designed using Primer Premier 5.0 Design software and checked against GenBank to avoid cross-reactivity with other known sequences. subsequently, using the TaKaRa One Step RNA PCR Kit (AMV) protocol, A RT-PCR mixture was prepared in a total volume of 50μl.…”

Section: Rna Isolation and Rt-pcr Assaymentioning

confidence: 99%

Expression of VEGF-C/VEGFR-3 in Human Laryngeal Squamous Cell Carcinomas and its Significance for Lymphatic Metastasis

Wang¹,

Yao²,

Li³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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VEGFc and VEGFR3 expression in human thyroid pathologies

Cited by 40 publications

References 20 publications

Papillary thyroid carcinoma involving cervical neck lymph nodes: correlations with lymphangiogenesis and ultrasound features

Papillary thyroid carcinoma involving cervical neck lymph nodes: correlations with lymphangiogenesis and ultrasound features

Immunodetection and quantification of vascular endothelial growth factor receptor‐3 in human malignant tumor tissues

Expression of VEGF-C/VEGFR-3 in Human Laryngeal Squamous Cell Carcinomas and its Significance for Lymphatic Metastasis

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