Vegetative Propagation of Mikania Glomerata: Micro- Propagation and Cuttings

Pereira, Ana Maria Soares; França, Suzelei de Castro; Câmara, Francisco Luiz Araújo

doi:10.17660/actahortic.1999.502.57

Cited by 3 publications

(5 citation statements)

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“…Role of cytokinins is obvious in bud proliferation and shoot multiplication (Aggarwal et al 1999;Pereira et al 1999;Kaur et al 2000;Sharma et al 2004;Kaloo et al 2013) and the stimulating effect of BA on multiple shoot formation has been reported for several medicinal and aromatic plant species (Pattnaik and Chand 1996;Saxena et al 1997;Khalafalla and Hattori 1999;Faisal et al 2006;Kaur et al 2007).…”

Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

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A protocol for in vitro propagation was developed for Viola pilosa, a plant of immense medicinal value. To start with in vitro propagation, the sterilized explants (buds) were cultured on MS basal medium supplemented with various concentrations of growth regulators. One of the medium compositions MS basal+0.5 mg/l BA+0.5 mg/l TDZ+0.5 mg/l GA 3 gave best results for in vitro shoot bud establishment. Although the problem of shoot vitrification occurred on this medium but this was overcome by transferring the vitrified shoots on MS medium supplemented with 1 mg/l BA and 0.25 mg/l Kn. The same medium was found to be the best medium for further in vitro shoot multiplication. 100 % root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 1 mg/l IBA. In vitro formed plantlets were hardened and transferred to soil with 83 % survival. Additionally, conservation of in vitro multiplying shoots was also attempted using two different approaches namely slowing down the growth at low temperature and cryopreservation following vitrification. At low temperature retrieval rate was better at 10°C than at 4°C after conservation of in vitro multiplying shoots. In cryopreservation-vitrification studies, the vitrified shoot buds gave maximum retrieval of 41.66 % when they were precooled at 4°C, while only 16.66 % vitrified shoots were retrieved from those precooled at 10°C. Genetic stability of the in vitro grown plants was analysed by RAPD and ISSR markers which indicated no somaclonal variation among in vitro grown plants demonstrating the feasibility of using the protocol without any adverse genetical effects.

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Section: Discussionmentioning

confidence: 99%

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Soni

Kaur

2013

Physiol Mol Biol Plants

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“…The kaurenoic acid derivative (diterpene) usually found in large amounts in this specie has not been detected ( Figure 1B). According to Pereira et al (1999), the greatest diffi culty in establishing this micropropagation protocol was the high levels of contamination by fungi and bacteria, thus requiring the cultivation of over 5000 explants and a 14 months period to achieve plantlet disinfestation. The mutagenic potential of numerous antibiotics has already been demonstrated, and could be related to the decrease of kaurenoic acid.…”

Section: Resultsmentioning

confidence: 99%

“…Cultivated specimens of Mikania glomerata Sprengel (Compositae) were obtained by propagation by cuttings and in vitro techniques processes as previously described by Pereira et al (1998) and Pereira et al (1999). Mikania glomerata Sprengel was collected in October 1994 from a single plant growing in the experimental area of the Universidade de Ribeirão Preto, São Paulo state, Brazil.…”

Section: Plant Materialsmentioning

confidence: 99%

Differences in secondary metabolites from leaf extracts of Mikania glomerata Sprengel obtained by micropropagation and cuttings

Contini¹,

Santos²,

Veneziani³

et al. 2006

Rev. bras. farmacogn.

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RESUMO: "Diferenças nos metabólitos secundários acumulados nos extratos das folhas deMikania glomerata Sprengel obtidos por micropropagação e estaquia". A análise realizada por CG dos extratos diclorometânicos das folhas de Mikania glomerata Sprengel mostrou que a planta propagada por estaca produziu cumarina e ácido caurenóico, enquanto que o material micropropagado acumulou apenas cumarina. Unitermos:Mikania glomerata, Asteraceae, micropropagação, estaquia, ácido caurenóico.ABSTRACT: GC analysis of the dichloromethane extracts obtained from cultivated specimens of Mikania glomerata Sprengel possibilited to verify that cuttings technique led to production of kaurenoic acid and coumarin while the same results have not been observed by propagation process using in vitro techniques.

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“…However, the endophytic DNA will be mixed into the host genome during extraction and bias the studies of the host plant. Various endophytes have been isolated in M. cordata [24], M. glomerate [26], and M. micrantha [25]. This suggests that endophytes removal becomes an obligatory step in related plant genomic research.…”

Section: Discussionmentioning

confidence: 99%

“…We performed the in vitro antibiotic-containing culture of M. cordata to decontaminate DNA sequences other than M. cordata. Micropropagation and tissue culture of Mikania species have been documented for M. glomerate [26] and M. micrantha [37]. Both exogenous and endogenous microbes have been noted as the greatest challenge in the micropropagation of M. glomerate [26].…”

Section: Discussionmentioning

confidence: 99%

Genome Survey Sequencing of In Vivo Mother Plant and In Vitro Plantlets of Mikania cordata

Hong

Huang

et al. 2020

Plants

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Mikania cordata, the only native congener of the invasive weed Mikania micrantha in China, is an ideal species for comparative study to reveal the invasion mechanism. However, its genome resources are lagging far behind its congener, which limits the comparative genomic analysis. Our goal is to characterize the genome of M. cordata by next-generation sequencing and propose a scheme for long-read genome sequencing. Previous studies have shown that the genomic resources of the host plant would be affected by the endophytic microbial DNA. An aseptic sample of M. cordata will ensure the proper genome in downstream analysis. Because endophytes are ubiquitous in the greenhouse-grown M. cordata, the in vitro culture with cefotaxime or timentin treatment was undertaken to obtain the aseptic plantlets. The in vivo mother plant and in vitro plantlets were used to survey the genome. The microbial contamination in M. cordata was recognized by blast search and eliminated from the raw reads. The decontaminated sequencing reads were used to predict the genome size, heterozygosity, and repetitive rate. The in vivo plant was so contaminated that microbes occupied substantial sequencing resources and misled the scaffold assembly. Compared with cefotaxime, treatment with timentin performed better in cultivating robust in vitro plantlets. The survey result from the in vitro plantlets was more accurate due to low levels of contamination. The genome size was estimated to be 1.80 Gb with 0.50% heterozygosity and 78.35% repetitive rate. Additionally, 289,831 SSRs were identified in the genome. The genome is heavily contaminated and repetitive; therefore, the in vitro culture technique and long-read sequencing technology are recommended to generate a high-quality and highly contiguous genome.

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Vegetative Propagation of Mikania Glomerata: Micro- Propagation and Cuttings

Cited by 3 publications

References 0 publications

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Rapid in vitro propagation, conservation and analysis of genetic stability of Viola pilosa

Differences in secondary metabolites from leaf extracts of Mikania glomerata Sprengel obtained by micropropagation and cuttings

Genome Survey Sequencing of In Vivo Mother Plant and In Vitro Plantlets of Mikania cordata

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