Vegetative Incompatibility in Botrytis

Korolev, Nadia; Elad, Y.

doi:10.1007/978-3-319-23371-0_4

Cited by 3 publications

(2 citation statements)

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“…The presence of different hosts in the vineyard ecosystem could possibly promote genetic variability in B . cinerea , which is known to possess a very high genetic diversity (De Miccolis Angelini et al ., 2016) that originates from several features, among which are heterokaryosis (Korolev and Elad, 2016) and sexual reproduction. Thanks to fusion between hyphae (anastomosis), different fungal colonies are capable of forming a heterokaryon, in which genetically different nuclei may coexist in a common cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

“…Thanks to fusion between hyphae (anastomosis), different fungal colonies are capable of forming a heterokaryon, in which genetically different nuclei may coexist in a common cytoplasm. Heterokaryosis is possible only between colonies that share identical recognition loci, labelled as heterokaryon incompatibility ( het ) or vegetative incompatibility ( vic ; Korolev and Elad, 2016). If two strains are not identical at these loci, their fusion is prevented and programmed cell death, visible as a black line between the colonies, rapidly occurs at the contact point of the hyphae (Fournier et al ., 2005).…”

Section: Introductionmentioning

confidence: 99%

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A molecular epidemiology study reveals the presence of identical genotypes on grapevines and ground cover weeds and the existence of separate genetic groups in a Botrytis cinerea population

et al. 2020

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Botrytis cinerea, the grey mould agent, is one of the most important pathogens of grapevine, due to the great yield losses caused and the economic costs related to disease control. Ground cover plants are assumed to have a role in the complex epidemiology of the pathogen, even if no information on the genetic variability of the strains is available. In this study, a molecular epidemiology approach, based on the comparative analysis of the nucleotide sequences of multiple genes (ITS1‐ITS2, G3PDH, NEP1, NEP2, BC‐hch, and sdhB), was used to evaluate whether B. cinerea isolated from herbaceous species contributes to grey mould diffusion on grapevines. From 330 samples collected in two vineyards in Lombardy, Italy, 63 B. cinerea strains were isolated from tissues of grapevine with symptoms (50 strains) and spontaneous ground cover plants (13 strains). Capsella bursa‐pastoris, Cardamine impatiens, Lamium purpureum, and Crepis tectorum were identified as novel B. cinerea hosts. Sequence analysis and phylogeny showed that the same genotypes were present on both grapevines and herbaceous plants, with no fitness (estimated from growth and sporulation on potato dextrose agar) or pathogenicity (on grapevine leaves and berries, and tomato leaves) penalties. This confirms that ground cover plants can be a source of inoculum for B. cinerea on grapevine. Moreover, phylogenetic analysis of the BC‐hch gene allowed the identification of two genetically distinct clusters, characterized by vegetative incompatibility and different distributions of the mating types, fitness, and pathogenicity. Therefore, B. cinerea seems to be composed of two diverging subpopulations that do not differ for host specialization.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A molecular epidemiology study reveals the presence of identical genotypes on grapevines and ground cover weeds and the existence of separate genetic groups in a Botrytis cinerea population

et al. 2020

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Genetic and pathogenic variation of Botrytis cinerea, the causal agent of grey mould on Panax ginseng in China

Wang

Liu

Chen

et al. 2022

Canadian Journal of Plant Pathology

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Morphological Diversity and Mycelial Compatibility of Botrytis pseudocinerea and Botrytis cinerea Isolated in Latvia

Dučkena,

Bimšteine,

Bankina

et al. 2024

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Botrytis is one the most economically important fungal taxa, comprising pathogens that infect a wide range of crops and causing gray mould. Two morphologically indistinguishable species, Botrytis cinerea and B. pseudocinerea, are significant pathogens found in close association in the same niches all over the world. Both species cause the same diseases in many plants; however, their mycelial compatibility in Latvia has not been reported. The aim of this study was to compare the morphological characteristics of B. cinerea and B. pseudocinerea isolates and determine their compatibility based on culturing on potato dextrose agar (PDA) media. A total of 113 isolates with the characteristic colony morphology of Botrytis spp. were obtained from four different host plants: tomato (Solanum lycopersicum), faba bean (Vicia faba), strawberry (Fragaria × ananassa), and cranberry (Vaccinium macrocarpon). The obtained isolates were divided into ten groups based on their morphological traits and hosts, and representatives from each group were chosen for further analyses. Botrytis spp. were identified at the species level using the DNA sequences of three protein-coding genes (RPB2, DNA-dependent RNA polymerase II; HSP60, heat shock protein 60; and G3PDH, glyceraldehyde 3-phosphate dehydrogenase). All ten isolates (five B. cinerea and five B. pseudocinerea) were pathogenic on wounded leaves and stems of ten-week-old tomato plants, and the pathogen was re-isolated and identified to fulfill Koch’s postulates. A high degree of phenotypic diversity was observed between and within B. cinerea and B. pseudocinerea. Mycelial compatibility was found to vary depending on the species, particular isolate, and host plant. This is the first report of B. pseudocinerea as a causal agent of gray mould on tomatoes in the Baltic region.

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Vegetative Incompatibility in Botrytis

Cited by 3 publications

References 57 publications

A molecular epidemiology study reveals the presence of identical genotypes on grapevines and ground cover weeds and the existence of separate genetic groups in a Botrytis cinerea population

A molecular epidemiology study reveals the presence of identical genotypes on grapevines and ground cover weeds and the existence of separate genetic groups in a Botrytis cinerea population

Genetic and pathogenic variation of Botrytis cinerea, the causal agent of grey mould on Panax ginseng in China

Morphological Diversity and Mycelial Compatibility of Botrytis pseudocinerea and Botrytis cinerea Isolated in Latvia

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