Genetic and pathogenic variation of <i>Botrytis cinerea</i>, the causal agent of grey mould on <i>Panax ginseng</i> in China

Wang, Rong; Liu, Kun; Chen, Bingwei; Ding, Wei; Li, Yong

doi:10.1080/07060661.2022.2067900

Cited by 3 publications

(1 citation statement)

References 44 publications

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“…cinerea is the causal agent of grey mould in more than 200 dicot plants, attacking tissues, such as stems, leaves, and fruits, as a necrotroph [ 5 ]. B. cinerea can affect economically important crops, such as vegetables (e.g., tomato, cucumber, and lettuce), ornamentals (e.g., rose and gerbera), bulbs (e.g., onion and ginseng), and fruits (e.g., grapevine, watermelon, and kiwifruit) [ 6 , 7 , 8 ]. In particular, B. cinerea can easily infect berries, such as strawberries, blueberries, raspberries, cranberries, and bilberry fruits, causing drastic losses after harvest [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

Orozco-Mosqueda

Kumar

Fadiji

et al. 2023

Plants

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Botrytis cinerea is the causal agent of grey mould and one of the most important plant pathogens in the world because of the damage it causes to fruits and vegetables. Although the application of botrycides is one of the most common plant protection strategies used in the world, the application of plant-beneficial bacteria might replace botrycides facilitating agroecological production practices. Based on this, we reviewed the different stages of B. cinerea infection in plants and the biocontrol mechanisms exerted by plant-beneficial bacteria, including the well-known plant growth-promoting bacteria (PGPB). Some PGPB mechanisms to control grey mould disease include antibiosis, space occupation, nutrient uptake, ethylene modulation, and the induction of plant defence mechanisms. In addition, recent studies on the action of anti-Botrytis compounds produced by PGPB and how they damage the conidial and mycelial structures of the pathogen are reviewed. Likewise, the advantages of individual inoculations of PGPB versus those that require the joint action of antagonist agents (microbial consortia) are discussed. Finally, it should be emphasised that PGPB are an excellent option to prevent grey mould in different crops and their use should be expanded for environmentally friendly agricultural practices.

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Section: Introductionmentioning

confidence: 99%

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

Orozco-Mosqueda

Kumar

Fadiji

et al. 2023

Plants

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Resistance of the Ginseng Gray Mold Pathogen, Botrytis cinerea, to Boscalid and Pyraclostrobin Fungicides in China

Yue,

Wang,

Liu

et al. 2024

Plant Disease

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Gray mold caused by Botrytis cinerea severely threatens the yield of ginseng (Panax ginseng). Various categories of fungicides have been utilized to control gray mold on this crop. In this study, the resistance of 102 isolates of B. cinerea from 11 commercial ginseng growing regions in China to fungicide was examined. A total of 32.4% were resistant to boscalid with EC50 values that ranged from 12.26 to 235.87 μg/ml, and 94.1% were resistant to pyraclostrobin with EC50 values that ranged from 5.88 to 487.72 μg/ml. Except for sdhA and sdhD, the amino acid substitutions of P225F, P225L, N230I, H272Y, and H272R in the sdhB subunit from 24 (4 S and 20 R), five (1 S and 4 R), one (S), 1 (R), and eight (4 S and 4 R) strains and the concurrent amino acid substitutions of G85A + I93V + M158V + V168I in the sdhC subunit from five (4 S and 1 R) strains were identified. A G143A substitution in cytochrome b was identified from 96 isolates that were resistant to pyraclostrobin and three that were sensitive to it. The Bcbi-143/144 intron was identified from the other three isolates sensitive to pyraclostrobin, but it was absent in the isolates that harbored the G143A mutation. The results showed that the populations of B. cinerea on ginseng have developed strong resistance to pyraclostrobin. Therefore, it is not recommended to continue using this fungicide to control gray mold on P. ginseng. Boscalid is still effective against most isolates, however, to prevent fungicide resistance, it is recommended to use mixtures with other categories of fungicides.

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A Portable Nucleic Acid Sensor Based on PCR for Simple, Rapid, and Sensitive Testing of Botrytis cinerea in Ginseng

Ren¹,

Wang²,

Chen³

et al. 2023

Plant Disease

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Botrytis cinerea is a ubiquitous pathogen that can infect at least 200 dicotyledonous plant species including many agriculturally and economically important crops. In Ginseng, the fungus may cause ginseng gray mold disease, causing great economic losses in ginseng industry. Therefore, the early detection of Botrytis cinerea in the process of ginseng production is necessary for the disease prevention and control of the pathogen’s spread. In this study, a polymerase chain reaction-nucleic acid sensor (PCR-NAS) rapid detection technique was established, and it can be used for field detection of B. cinerea through anti-pollution design and portable integration. The present study showed that the sensitivity of PCR-NAS technology is 10 times higher than that of traditional PCR-electrophoresis, and there is no need for expensive detection equipment or professional technicians. The detection results of nucleic acid sensors can be read by the naked eye under 3 minutes. Meanwhile, the technique has high specificity for the detection of B. cinerea. The testing of 50 field samples showed that the detection results of PCR-NAS were consistent with those of the real-time quantitative PCR (qPCR) method. The PCR-NAS technique established in this study can be used as a novel nucleic acid field detection technique, and it has a potential application in the field detection of B. cinerea to achieve early warning of the pathogen infection.

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Genetic and pathogenic variation of Botrytis cinerea, the causal agent of grey mould on Panax ginseng in China

Cited by 3 publications

References 44 publications

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

Agroecological Management of the Grey Mould Fungus Botrytis cinerea by Plant Growth-Promoting Bacteria

Resistance of the Ginseng Gray Mold Pathogen, Botrytis cinerea, to Boscalid and Pyraclostrobin Fungicides in China

A Portable Nucleic Acid Sensor Based on PCR for Simple, Rapid, and Sensitive Testing of Botrytis cinerea in Ginseng

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