Vegetable Stain as an Alternative to H&amp;E in Exfoliative Cytology

Singnarpi, Sherin; Ramani, Pratibha; Natesan, Anuja; Sherlin, Herald J; Gheena, S; Giffrina,; Abhilasha,; Don,; Archana, .

doi:10.4172/2157-7099.1000469

Cited by 8 publications

(9 citation statements)

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“…Also, it has been used in staining of helminth parasites [3,10] and ova of intestinal nematodes [2]. Recently, Singnarpi et al [1] have applied beetroot in cytology for staining of buccal smears and Das et al [11] have applied beetroot dye as a fluorescent dye in tissue staining. Although beetroot is generally used as food colorant, in staining helminth parasites, and in cytology, little knowledge has been uncovered about the usefulness of beetroot extracts as a potent stain in histology.…”

Section: Introductionmentioning

confidence: 99%

“…It belongs to the family Chenopodae [1][2][3] and order Caryophyllales [4,5]. Beetroot has been used as food [6] and as medicinal plant for the treatment of various ailments because of its lipotropic, antioxidant, and antitumor properties [1]. Beetroot has also been widely used industrially as food colorant due to its red color from the pigment betalain mainly from betanin, betanidin, and betaxanthin [4].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spectrophotometry, Physiochemical Properties, and Histological Staining Potential of Aqueous and Ethanol Extracts of Beetroot on Various Tissues of an Albino Rat

Udonkang¹,

Inyang²,

Ukorebi³

et al. 2018

Biomed Hub

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Background and Aims: Natural dyes and pigments are nontoxic, ecofriendly alternatives to synthetic counterparts and beetroot is one such natural dye. The red color of beetroot derived from betalain pigments confers great advantage to this plant. In this study, the physiochemical and spectrophotometric characteristics of beetroot as well as the histological staining potential of various tissues were carried out to determine its tissue specificity. Methods: The aqueous and ethanol extracts of beetroot were prepared using distilled water and 95% ethanol, respectively. Spectrophotometry, pH, and concentration of both extracts were determined before histological staining with 10% neutral-buffered formalin-fixed, paraffin-wax-embedded tissue sections. Stained sections were viewed with a photomicroscope. Results: The aqueous and ethanol extracts of beetroot were slightly acidic and soluble at concentrations of 381.5 mg/100 g and 253.7 mg/100 g fresh beetroot sample, respectively. Both extracts consist of three betalain pigments with absorbances at different spectrophotometric wavelengths, namely betaxanthins (475 nm), betanin (525 nm), and betanidin (575 nm). The maximum absorbance was 0.925 and 0.615 for the aqueous and ethanol extracts, respectively, at a peak wavelength of 525 nm for each extract. Both extracts stained various tissue structures such as muscles, mucins, red blood cells, keratin, and nerve fibers. Conclusion: Thus, beetroot stain is slightly acidic, contains betalain pigments, stains basic histological tissue structures, and can be used as an ecofriendly alternative to hematoxylin and eosin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Spectrophotometry, Physiochemical Properties, and Histological Staining Potential of Aqueous and Ethanol Extracts of Beetroot on Various Tissues of an Albino Rat

Udonkang¹,

Inyang²,

Ukorebi³

et al. 2018

Biomed Hub

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“…This natural extract was also used in fluorescence cell imaging and textile dyeing (Das, A. et al, 2017;AL-Khateeb et al, 2019). Beet root is also reported to stain buccal smear in exfoliative microscopic anatomy (Singnarpi, S. et al, 2017). Thus the present work was attempted to use the extract of Beta vulgaris L. to stain the fungal cells and study their structural features.…”

Section: Resultsmentioning

confidence: 99%

Use of the Natural Pigments of Red Beet Root Pomace (Beta vulgaris L.) to Develop a Mycological Stain: An eco-friendly Alternative Substitute

Sutradhar¹,

Bhattacharya²

2021

JSR

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The deleterious effects of synthetic dyes evoked great enthusiasm in many scientists to search for eco-friendly natural colorants for morphological study and identification of microorganisms. The aim of the study was to develop a mycological staining reagent from the red pigments extracted from beet root pomace (Beta vulgaris L.). Beet root rich in water soluble betalains was extracted with distilled water at 40ºC.The conventional easy and economical wet mount procedure was followed to prepare the fungal slides. Glycerol was used as a hygroscopic agent which did not affected the colour of the extract. In the current investigation the extract was evaluated as a staining reagent to study the morphology of the test fungal samples viz. Rhizopus sp., Microsporum gypseum and Aspergillus niger. The study revealed that the use of citric acid buffer intensified its red colour potentially at pH 5.This novel staining reagent was proved to be reliable and safe with satisfactory visual clarity of the characteristic fungal structures. In conclusion, this ecofriendly alternative method of staining by such non-toxic vegetal active principle provided a promising way to support environmental sustainability.

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“…[ 4 5 6 ] These pigments are generally tasteless and odorless with substantial color stability[ 6 ] and have thus been widely promoted as safe-to-consume coloring agents in foods and beverages. [ 5 7 ] Furthermore, various researchers have successfully demonstrated that anthocyanins obtained from Hibiscus sabdariffa (Hibiscus), R. indica (Rose) and Bougainvillea glabra ( Bougainvillea ) and beetroot[ 8 9 10 ] can be effectively used for histological staining.…”

Section: Introductionmentioning

confidence: 99%

Toward developing natural histologic stains using anthocyanins

Sachdev

Chettiankandy

Sonawane

et al. 2021

Journal of Oral and Maxillofacial Pathology

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Introduction: In the present-day scenario, wherein histotechnological laboratory personnel come into contact with numerous hazardous chemicals every day, laboratories are emphasizing on development of safer and environment-friendly alternatives globally which are easily available and feasible. In this context, we have attempted to utilize anthocyanins, a family of pigments naturally occurring in fruits and flowers and which are already used as natural food colorants, for assessing their utility as histological stains. Materials and methods: Juices of thin consistency from well-ripened pomegranates were obtained by using a juicer with blender functions. Formalin-fixed paraffin-embedded 4 μm thin sections were immersed in this solution for 2 h, during which the solution was periodically monitored. Several modifications were attempted such as the addition of Citrus limon (lemon) extract or acetic acid, change in orientation of the slides and refrigeration of the solution during the staining procedure. Results: A peculiar pattern of reasonably diagnostic staining was observed in which the basal and suprabasal cells, basement membrane, inflammatory cells and collagen fibers stained prominently with a magenta color. The addition of lemon, horizontal orientation of slides and refrigeration each separately led to an improvement in staining characteristics. The addition of 4% acetic acid and refrigeration of the solution each led to an improvement in longevity of the staining solution. Conclusion: Anthocyanins could potentially be used as viable histological stains having advantages of availability, feasibility, color stability and nontoxicity, although numerous modifications to improve longevity of staining solution and staining characteristics are warranted by further research for which the present study could serve as a pretext.

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Vegetable Stain as an Alternative to H&E in Exfoliative Cytology

Cited by 8 publications

References 0 publications

Spectrophotometry, Physiochemical Properties, and Histological Staining Potential of Aqueous and Ethanol Extracts of Beetroot on Various Tissues of an Albino Rat

Spectrophotometry, Physiochemical Properties, and Histological Staining Potential of Aqueous and Ethanol Extracts of Beetroot on Various Tissues of an Albino Rat

Use of the Natural Pigments of Red Beet Root Pomace (Beta vulgaris L.) to Develop a Mycological Stain: An eco-friendly Alternative Substitute

Toward developing natural histologic stains using anthocyanins

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