Vectorial transport of bile salts across MDCK cells expressing both rat Na⁺-taurocholate cotransporting polypeptide and rat bile salt export pump

Abstract

transport of bile salts across MDCK cells expressing both rat Na ϩ -taurocholate cotransporting polypeptide and rat bile salt export pump.

The Role of the Sodium-Taurocholate Cotransporting Polypeptide (NTCP) and of the Bile Salt Export Pump (BSEP) in Physiology and Pathophysiology of Bile Formation

“…Furthermore, transport in a vesicular system can be measured as uptake. for all conjugated bile salts tested [223]. A similar study with human NTCP and BSEP expressed in LLC-PK1 cells identified again cholate, chenodeoxycholate and ursodeoxycholate as BSEP substrates together with all conjugated bile salts tested [221].…”

“…In contrast, BSEP expressed in SF9 cell vesicles does not transport cholate [221,240], which parallels the virtual absence of unconjugated bile acids in the bile from patients with a defect in bile acid conjugation [37]. Neither the human nor the rat orthologue of Bsep transport lithocholate [221,223]. …”

“…Cholate (X. laevis) [273] Cholate (COS-7 transient) [168] Cholate (MDCK) [223] Chenodeoxycholate (MDCK) [223] Ursodeoxycholate (MDCK) [223] Glycocholate (X. laevis) [273] Glycocholate (CHO) 27 [273] Glycocholate (COS-7 transient) [168] Glycocholate (CHO) [31] Glycocholate (HeLa) [111] Glycocholate (MDCK) [223] Glycochenodeoxycholate (MDCK) [223] Glycoursodeoxycholate (MDCK) [223] Tauromurocholate (HeLa) [111] Taurohyodeoxycholate (HeLa) 7 [111] Taurolagodeoxycholate (HeLa) [111] Taurodeoxycholate (HeLa) 7 [111] Tauro-allo-cholate (CHO) [219] Taurocheondeoxchycholate (X. laevis) [273] Taurochenodeoxycholate (CHO) 5 [273] Taurochenodeoxycholate (MDCK) [223] Tauroursodeoxycholate (X. laevis) [273] Tauroursodeoxycholate (CHO) 14 [273] Tauroursodeoxycholate (MDCK) [223] Bile salt and steroid metabolites Estrone-3-sulfate (X. laevis) [273] Estrone-3-sulfate (CHO) 27 [273] Dehydroepiandosterone sulfate (HepG2, EGFP-tagged) [178] Others Sulfobromophthalein (X. laevis) [214] Thyroxine ( ONO-1301 (not specified) [309] Bamet-R2 (CHO) *)…”

Bile acid transporters

“…NTCP's major physiological substrates include all the major glycine and taurine-conjugated bile acids (48)(49)(50)(51)(52). Depending on the structure of the bile acid and NTCP ortholog, unconjugated bile acids are moderate or weak substrates ( 50,53,54 ) and sulfated bile acids appear to be only weakly transported ( 55 ). In contrast to NTCP, members of the OATP of bile acids ( 1 ).…”

Bile Acids Act as Soluble Host Restriction Factors Limiting Cytomegalovirus Replication in Hepatocytes

“…These cells also failed to reduce MCMV-dependent luciferase activity upon bile acid treatment (data not shown). To further substantiate the hypothesis that the import of bile acids is a necessary prerequisite for antiviral activity, an experiment was designed based on the observation that TCDC inhibits MCMV, whereas TUDC does not, and the knowledge that both compounds compete for the same bile acid transporters (50,51). Graded TUDC concentrations counteracted the antiviral activity of TCDC in a dose-dependent manner (Fig.…”