VDAC contributes to mRNA levels in Saccharomyces cerevisiae cells by the intracellular reduction/oxidation state dependent and independent mechanisms

Abstract

Available data suggest that voltage-dependent anion selective channel (VDAC) constitutes an important component of a cellular regulatory mechanism based on the intracellular reduction/oxidation (redox) state. Here, using quantitative RT-PCR, we demonstrated that depletion of VDAC1 (termed here VDAC) in Saccharomyces cerevisiae cells distinctly affected levels of mRNAs encoding nuclear proteins sensitive to changes of the intracellular redox state including the nuclear transcription factors important for adaptation to the redox state and proteins involved in communication between mitochondria and the nucleus. We also revealed that the changes of the studied protein transcript levels generally correlated with changes of the intracellular redox state although VDAC appears also to affect mRNA levels by a mechanism not based on changes of the intracellular redox states. Thus, VDAC seems to be an important element of the intracellular signaling network.

Voltage-dependant anion channels: Novel insights into isoform function through genetic models

“…Other processes where a role for YVDAC1 has been proposed include as a channel for metabolites such as NADH, and as a conduit for reactive oxygen species (ROS) signaling via redox regulation and consequent regulation of mRNA and protein levels in the cell. [20,21,22,23]. YDAC1 has been hypothesized to control cellular redox states by the transport of ROS species such as the superoxide anion ($O_2^{-}$) across the MOM, and this in term is known to affect cellular mRNA and protein levels (reviewed in Ref.…”

Arabidopsis Mitochondrial Voltage-Dependent Anion Channels Are Involved in Maintaining Reactive Oxygen Species Homeostasis, Oxidative and Salt Stress Tolerance in Yeast

“…Another possibility, especially in case of salt stress, could be that some other transcriptional or post-translational mechanism might be controlling VDAC activity. It has been proved, in S. cerevisiae, VDAC has a role in maintaining the cytosolic redox state that regulates the expression of genes (Galganska et al, 2010b). So the salt stress mediated perturbation may lead to transcriptional regulation of VDAC transcripts.…”

Carbon dioxide inhibits COVID-19-type proinflammatory responses through extracellular signal-regulated kinases 1 and 2, novel carbon dioxide sensors

“…RNA extraction with TRI reagent (Sigma-Aldrich) and reverse transcription using a RevertAid H Minus First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1631) were conducted according to the manufacturers' protocols and described previous particulars [12]. PowerUp™ SYBR ® Green Master Mix (Thermo Fisher Scientific, A25742) and StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) were used to perform qPCR with primers specific to ACE2 (ACC AGT GGA TGA AAA AGT GGTG and AGA AAC ATG GAA CAG AGA TGCG), GAPDH (GTC TCC TCT GAC TTC AAC AGCG and ACC ACC CTG TTG CTG TAG CCAA) and ACTB (CAC CAT TGG CAA TGA GCG GTTC and AGG TCT TTG CGG ATG TCC ACGT).…”