Arabidopsis Mitochondrial Voltage-Dependent Anion Channels Are Involved in Maintaining Reactive Oxygen Species Homeostasis, Oxidative and Salt Stress Tolerance in Yeast

Abstract

Voltage-dependent anion channels (VDACs) are conserved proteins of the mitochondria. We have functionally compared Arabidopsis VDACs using Saccharomyces cerevisiae Dpor1 and M3 yeast system. VDAC (1, 2, and 4) were able to restore Dpor1 growth in elevated temperature, in oxidative and salt stresses, whereas VDAC3 only partially rescued Dpor1 in these conditions. The ectopic expression of VDAC (1, 2, 3, and 4) in mutant yeast recapitulated the mitochondrial membrane potential thus, enabled it to maintain reactive oxygen species homeostasis. Overexpression of these VDACs (AtVDACs) in M3 strain did not display any synergistic or antagonistic activity with the native yeast VDAC1 (ScVDAC1). Collectively, our data suggest that Arabidopsis VDACs are involved in regulating respiration, reactive oxygen species homeostasis, and stress tolerance in yeast.

Acute environmental temperature variation affects brain protein expression, anxiety and explorative behaviour in adult zebrafish

“…The impact of acute heat stress on anion transport and porins is mainly due to the decreased expression of voltage-dependent anion channel VDAC proteins of the mitochondrial outer membrane that regulate the influx of respiratory substrates ADP and Pi into the mitochondrion and ATP release into the cytosol under both physiological and pathological conditions. In many eukaryotic cells, VDACs are involved in maintaining cell homeostasis under different stress responses, such as oxidative, salt and temperature stress 102 . In mice, decreased VDAC expression is associated with decreased ATP synthesis and deficits in learning and synaptic plasticity 103 .…”

The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration

“…Several clues suggest that VDACs are involved in the plant response to abiotic and biotic stresses [ 6 , 7 , 8 , 9 , 10 ]. The modification of the membrane lipid composition is a hallmark of plant stress responses and its effect on mitochondria function has long been recognized [ 11 , 12 , 13 ].…”

Overexpression of a Voltage-Dependent Anion-Selective Channel (VDAC) Protein-Encoding Gene, MsVDAC, from Medicago sativa Confers Cold and Drought Tolerance to Transgenic Tobacco

“…In plants, VDACs not only play a role in metabolite transport but are also involved in programmed cell death triggered in response to abiotic and biotic stresses [27]. Although many plant VDAC genes that participate in temperature, oxidative stress and salt stress responses have been characterized [9,28], there have been no studies on these genes in alfalfa. In this study, a VDAC gene from alfalfa named MsVDAC was cloned and functionally characterized.…”

“…The content of reduced glutathione (GSH) was fluorometrically estimated [23]. The content of soluble sugar (SS) was determined using the methods of Dreywood [9], and the soluble protein (SP) content was determined by the Bradford method [24]. The contents of free proline were determined according to the ninhydrin method [25].…”

“…Cell death that occurred in the tobacco cell line BY2 and in the leaves of tobacco plants heterologously expressing a rice VDAC demonstrated that the ratio of VDAC and hexokinase expression and the interaction of these proteins play a key role in modulating senescence-related pathways in plants [8]. Arabidopsis VDACs have been shown to participate in the regulation of respiration, the balance of ROS, and the stress tolerance of yeast [9]. AtVDAC1 is not only necessary for normal growth but also important for disease tolerance via its ability to regulate the generation of hydrogen peroxide (H 2 O 2 ).…”