Vasotocin and isotocin regulate aquaporin 1 function in the sea bream

Martos-Sitcha, Juan Antonio; Campinho, Marco A.; Mancera, Juan Miguel; Martı́nez-Rodrı́guez, Gonzalo; Fuentes, Juan

doi:10.1242/jeb.114546

Cited by 24 publications

(16 citation statements)

References 76 publications

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“…Methodology for the ex vivo opercular epithelia preparation followed our current methods. 45 Fish were anaesthetized with 2-phenoxyethanol (1 : 2000 v/v), sacrificed by decapitation and the cranium was cut longitudinally. The gills and other tissue remains were removed carefully and the epithelial skin covering the opercular bone were dissected out and transferred to fresh-gassed saline (99.7 : 0.3 O 2 /CO 2 ) with the following composition (all values in mM): NaCl, 160; MgSO 4 , 0.93; NaH 2 PO 4 , 3.0; CaCl 2 , 1.5; NaHCO 3 , 17.85; KCl, 3.0; glucose, 5.5; HEPES (pH 7.8), 5.0.…”

Section: Methodsmentioning

confidence: 99%

The P-type ATPase inhibiting potential of polyoxotungstates

et al. 2018

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Section: Methodsmentioning

confidence: 99%

The P-type ATPase inhibiting potential of polyoxotungstates

et al. 2018

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“…Vasopressin is important for water balancing via the control of antidiuresis and regulation of blood pressure and plays a role in influencing social behaviors such as territorial defense, aggression, and also pair bonding (5–8). Across vertebrates, oxytocin/vasopressin-like peptide homologs appear to have conserved physiological (9, 10) and behavioral functions (11–15).…”

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Oxytocin/vasopressin-like peptide inotocin regulates cuticular hydrocarbon synthesis and water balancing in ants

Koto

Motoyama

Tahara

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Oxytocin/vasopressin-like peptides are important regulators of physiology and social behavior in vertebrates. However, the function of inotocin, the homologous peptide in arthropods, remains largely unknown. Here, we show that the level of expression of inotocin and inotocin receptor are correlated with task allocation in the antCamponotus fellah. Both genes are up-regulated when workers age and switch tasks from nursing to foraging. in situ hybridization revealed thatinotocin receptoris specifically expressed in oenocytes, which are specialized cells synthesizing cuticular hydrocarbons which function as desiccation barriers in insects and for social recognition in ants. dsRNA injection targetinginotocin receptor, together with pharmacological treatments using three identified antagonists blocking inotocin signaling, revealed that inotocin signaling regulates the expression ofcytochrome P450 4G1(CYP4G1) and the synthesis of cuticular hydrocarbons, which play an important role in desiccation resistance once workers initiate foraging.

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“…The seawater teleost rectum also plays a role in water absorption. For instance, in the sea bream (Sparus aurata) and Japanese eel (Anguilla japonica), fluid absorption by the rectum also occurs via aquaporins, but the rate of absorption is less than in the intestine (8,15,28,36). The rectum of the Gulf toadfish (Opsanus beta) also expresses intestinal ion transporters and enzymes involved in solute-coupled water absorption, such as SLC26a6, the basolateral electrogenic Na ϩ /HCO 3 Ϫ -cotransporter [NBCe1 (SLC4a4)], apical VHA, and cytosolic carbonic anhydrase (CA C ) (20,22,42,49).…”

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The role of the rectum in osmoregulation and the potential effect of renoguanylin on SLC26a6 transport activity in the Gulf toadfish (Opsanus beta)

Ruhr

Takei

Grosell

2016

American Journal of Physiology-Regulatory, Integrative and Comp

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Ruhr IM, Takei Y, Grosell M. The role of the rectum in osmoregulation and the potential effect of renoguanylin on SLC26a6 transport activity in the Gulf toadfish (Opsanus beta). Am J Physiol Regul Integr Comp Physiol 311: R179 -R191, 2016. First published March 30, 2016 doi:10.1152/ajpregu.00033.2016.-Teleosts living in seawater continually absorb water across the intestine to compensate for branchial water loss to the environment. The present study reveals that the Gulf toadfish (Opsanus beta) rectum plays a comparable role to the posterior intestine in ion and water absorption. However, the posterior intestine appears to rely more on SLC26a6 (a HCO 3 Ϫ /Cl Ϫ antiporter) and the rectum appears to rely on NKCC2 (SLC12a1) for the purposes of solute-coupled water absorption. The present study also demonstrates that the rectum responds to renoguanylin (RGN), a member of the guanylin family of peptides that alters the normal osmoregulatory processes of the distal intestine, by inhibited water absorption. RGN decreases rectal water absorption more greatly than in the posterior intestine and leads to net Na ϩ and Cl Ϫ secretion, and a reversal of the absorptive short-circuit current (ISC). It is hypothesized that maintaining a larger fluid volume within the distal segments of intestinal tract facilitates the removal of CaCO3 precipitates and other solids from the intestine. Indeed, the expression of the components of the Cl Ϫ -secretory response, apical CFTR, and basolateral NKCC1 (SLC12a2), are upregulated in the rectum of the Gulf toadfish after 96 h in 60 ppt, an exposure that increases CaCO3 precipitate formation relative to 35 ppt. Moreover, the downstream intracellular effects of RGN appear to directly inhibit ion absorption by NKCC2 and anion exchange by SLC26a6. Overall, the present findings elucidate key electrophysiological differences between the posterior intestine and rectum of Gulf toadfish and the potent regulatory role renoguanylin plays in osmoregulation. water secretion; Cl Ϫ secretion; HCO 3 Ϫ secretion; intestine; marine teleost; CFTR SEAWATER TELEOST FISH LIVE IN an environment that is hypertonic to their blood plasma, resulting in continual water loss through the gills (34). To compensate for this, seawater teleosts drink copious volumes of water that are continually desalinated by the esophagus and gastrointestinal tract to produce a fluid that is roughly isotonic to blood plasma (17, 34). The constant absorption of ions, by the intestine, is critical as it lowers luminal osmolality and allows for solute-coupled water absorption, and, thereby, compensating for branchial water loss (45,46 (53), a process coupled to the ion transport mechanisms described above. The seawater teleost rectum also plays a role in water absorption. For instance, in the sea bream (Sparus aurata) and Japanese eel (Anguilla japonica), fluid absorption by the rectum also occurs via aquaporins, but the rate of absorption is less than in the intestine (8,15,28,36). The rectum of the Gulf toadfish (Opsanus beta) also expres...

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Vasotocin and isotocin regulate aquaporin 1 function in the sea bream

Cited by 24 publications

References 76 publications

The P-type ATPase inhibiting potential of polyoxotungstates

The P-type ATPase inhibiting potential of polyoxotungstates

Oxytocin/vasopressin-like peptide inotocin regulates cuticular hydrocarbon synthesis and water balancing in ants

The role of the rectum in osmoregulation and the potential effect of renoguanylin on SLC26a6 transport activity in the Gulf toadfish (Opsanus beta)

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