Vasoactive intestinal peptide (VIP)-like immunoreactivity in the human sympathetic ganglia

Järvi, Riitta; Helén, Pauli; Hervonen, Antti; Pelto‐Huikko, Markku

doi:10.1007/bf00508311

Cited by 19 publications

(6 citation statements)

References 19 publications

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“…In this study, single VIP-IR neurons were TH-negative and ChAT-negative, whereas TH-immunoreactivity in VIP-immunoreactive neurons of the human www.fhc.viamedica.pl lumbar ganglia [26] and porcine SCG [23,25] were not identified. The coexistence of DBH-and VIP-IR neurons was not identified in the paravertebral and prevertebral sympathetic ganglia of the cat [27], while the colocalization of DBH-and VIP-immunoreactivity was indicated only in neurons of thoracic ganglia in the pig [24].…”

Section: Discussioncontrasting

confidence: 60%

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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Introduction. Greater splanchnic nerve (GSN) is by far the largest of the splanchnic nerves and connects the paravertebral and prevertebral ganglia to transmit the majority of nociceptive information from the viscera. Despite its importance, the immunohistochemical features of the porcine GSN neurons have not yet been examined. Therefore, the aim of the study was to investigate the neurochemistry of the porcine GSN neurons and to compare their neurochemical coding with those of the paravertebral and prevertebral ganglia. Material and methods. Four gilts of Large White Polish breed were examined in this study. Antibodies to tyrosine hydroxylase (TH), dopamine b-hydroxylase (DBH), choline acetyltransferase (ChAT), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), somatostatin (SOM), galanin (GAL), methionine-enkephalin (MET), calcitonin gene-related peptide (CGRP), and substance P (SP) were used for immunohistochemical detection of classical neurotransmitters marker enzymes and neuropeptides in neuronal cell bodies of the GSN. Results. Double-labeling immunofluorescence revealed that virtually all GSN neurons exhibited the presence of catecholamine-synthesizing enzymes (TH/DBH-positive) and subpopulations of neurons contained immunoreactivity to NPY, VIP, SOM, GAL and MET. However, CGRP and SP-immunoreactivity were not observed in neuronal somata. Conclusions. Our data strongly suggest that the general immunohistochemical characterization of ganglion cells in the porcine greater splanchnic nerve is similar to that of the prevertebral ganglia (e.g. celiacomesenteric ganglion).

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Section: Discussioncontrasting

confidence: 60%

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Nourinezhad

Podlasz

Wąsowicz

2018

Folia Histochem Cytobiol.

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“…It has been demonstrated previously that VIP-IR is present in part of the cholinergic neurons of h u m a n paravertebral ganglia (Jarvi et al, 1988), which innervate the sweat glands (Lundberg et al, 1980;Vaalasti et al, 1985). Thus ENK-IR nerve terminals are not, at least directly, involved with vascular control, which is effected by the NPY-IR and TH-IR neurons, but may have a function in the regulation of sweating and body temperature via cholinergic neurons.…”

Section: Discussionmentioning

confidence: 99%

Localization of neuropeptide Y in human sympathetic ganglia: Correlation with met-enkephalin, tyrosine hydroxylase and acetylcholinesterase

Järvi

Pelto‐Huikko

1990

Histochem J

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The relationships of immunoreactive neuropeptide Y, enkephalin and tyrosine hydroxylase, on the one hand, and acetylcholinesterase histochemical activity, on the other, were studied in human lumbar sympathetic ganglia. Two thirds of the ganglion cells contained immunoreactive neuropeptide Y. Electron microscopically the immunoreaction was localized in the Golgi apparatus and in large dense-cored vesicles in the nerve endings. Most of the neuropeptide-containing neurons and nerve fibres were also reactive for tyrosine hydroxylase. Nerve fibres reactive for neuropeptide Y were found around ganglion cells regardless of their transmitter contents, whereas enkephalin-reactive nerve terminals surrounded only acetylcholinesterase-containing neurons. The results demonstrate that neuropeptide Y is colocalized with noradrenaline in most of the human sympathetic neurons and that the nerve fibres may innervate selectively the noradrenergic and cholinergic subpopulations of ganglion cells depending on the transmitters of the nerves.

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“…Cholinergic marker has been documented in the STG of the sow (Panu et al, 2001;Ragionieri et al, 2008), cat (Lindh et al, 1989), rat (Morales et al, 1995;Schafer et al, 1997Schafer et al, , 1998, guinea-pig (Elfvin et al, 1993) and man (Jarvi et al, 1989;Masliukov and Timmermans, 2004). The principal function of cholinergic innervation in the uro-genital system is the presynaptic inhibition of the adrenergic excitatory neurotransmission (Sj€ ostrand, 1965;Sann et al, 1995;Anderson et al, 1997;Saenz de Tejada et al, 2000).…”

Section: Vacht-immunoreactivitymentioning

confidence: 99%

Sensory and Autonomic Neurons Project Both to the Smooth Retractor Penis and to the Striated Bulbospongiosus Muscles. Neurochemical Features of the Sympathetic Subset

Botti

Gazza

Ragionieri

et al. 2012

The Anatomical Record

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Aim of the present study was to verify, by means of double retrograde neuronal tracers technique, the hypothesis that a subpopulation of sensory and autonomic neurons send collateral axons to both smooth and striated genital muscles. We also wanted to define the neurochemical content of the eventually retrogradelly double labeled (RDL) neurons in the sympathetic trunk ganglia (STG). We used six intact pigs and we injected the tracer Diamidino Yellow (DY) in the smooth left retractor penis muscle (RPM) and the tracer Fast Blue (FB) in the striated left bulbospongiosus muscle (BSM). Rare (2 6 0.6) RDL neurons were found in the ipsilateral S2 spinal ganglion (SG), 220 6 42 in the ipsilateral STGs, from L3 to S3, 19 6 15 in the contralateral S1-S2 ones and 22 6 5 in the bilateral caudal mesenteric ganglia (CMG). The RDL neurons of the STG were IR for TH (85 6 13%), DbH (69 6 17%), NPY (69 6 23%), nNOS (60 6 11%), LENK (54 6 19%), VIP (53626%), SOM (40 6 8%), CGRP (34 6 12%), SP (31 6 16%), and VAChT (28 6 3%). Our research highlights the presence of sensory and sympathetic neurons with qualitatively different neurochemical content sending axons both to the smooth RPM and to the striated BSM of the pig. These RDL neurons are likely to project to the smooth vasal musculature to create the ideal physiological conditions in which these muscles can optimize the erectile function.

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Vasoactive intestinal peptide (VIP)-like immunoreactivity in the human sympathetic ganglia

Cited by 19 publications

References 19 publications

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Immunohistochemical characteristics of neurons in the ganglia of the greater splanchnic nerve of the pig

Localization of neuropeptide Y in human sympathetic ganglia: Correlation with met-enkephalin, tyrosine hydroxylase and acetylcholinesterase

Sensory and Autonomic Neurons Project Both to the Smooth Retractor Penis and to the Striated Bulbospongiosus Muscles. Neurochemical Features of the Sympathetic Subset

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